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Various extraction methods for obtaining stilbenes from grape cane of Vitis vinifera L

I. Soural, N. Vrchotová, J. Tříska, J. Balík, Š. Horník, P. Cuřínová, J. Sýkora,

. 2015 ; 20 (4) : 6093-112. [pub] 20150408

Language English Country Switzerland

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc16010354

Grape cane, leaves and grape marc are waste products from viticulture, which can be used to obtain secondary stilbene derivatives with high antioxidant value. The presented work compares several extraction methods: maceration at laboratory temperature, extraction at elevated temperature, fluidized-bed extraction, Soxhlet extraction, microwave-assisted extraction, and accelerated solvent extraction. To obtain trans-resveratrol, trans-ε-viniferin and r2-viniferin from grape cane of the V. vinifera variety Cabernet Moravia, various conditions were studied: different solvents, using powdered versus cut cane material, different extraction times, and one-step or multiple extractions. The largest concentrations found were 6030 ± 680 µg/g dry weight (d.w.) for trans-resveratrol, 2260 ± 90 µg/g d.w. for trans-ε-viniferin, and 510 ± 40 µg/g d.w. for r2-viniferin. The highest amounts of stilbenes (8500 ± 1100 µg/g d.w.) were obtained using accelerated solvent extraction in methanol.

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$a Soural, Ivo $u Department of Post-Harvest Technology of Horticultural Products, Faculty of Horticulture, Mendel University in Brno, Valtická 337, Lednice 69144, Czech Republic. ivo.soural@mendelu.cz.
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$a Various extraction methods for obtaining stilbenes from grape cane of Vitis vinifera L / $c I. Soural, N. Vrchotová, J. Tříska, J. Balík, Š. Horník, P. Cuřínová, J. Sýkora,
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$a Grape cane, leaves and grape marc are waste products from viticulture, which can be used to obtain secondary stilbene derivatives with high antioxidant value. The presented work compares several extraction methods: maceration at laboratory temperature, extraction at elevated temperature, fluidized-bed extraction, Soxhlet extraction, microwave-assisted extraction, and accelerated solvent extraction. To obtain trans-resveratrol, trans-ε-viniferin and r2-viniferin from grape cane of the V. vinifera variety Cabernet Moravia, various conditions were studied: different solvents, using powdered versus cut cane material, different extraction times, and one-step or multiple extractions. The largest concentrations found were 6030 ± 680 µg/g dry weight (d.w.) for trans-resveratrol, 2260 ± 90 µg/g d.w. for trans-ε-viniferin, and 510 ± 40 µg/g d.w. for r2-viniferin. The highest amounts of stilbenes (8500 ± 1100 µg/g d.w.) were obtained using accelerated solvent extraction in methanol.
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$a Vrchotová, Naděžda $u Global Change Research Centre, Academy of Sciences of the Czech Republic, v. v. i., Branišovská 31, České Budějovice 37005, Czech Republic. vrchotova.n@czechglobe.cz.
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$a Tříska, Jan $u Global Change Research Centre, Academy of Sciences of the Czech Republic, v. v. i., Branišovská 31, České Budějovice 37005, Czech Republic. triska.j@czechglobe.cz.
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$a Balík, Josef $u Department of Post-Harvest Technology of Horticultural Products, Faculty of Horticulture, Mendel University in Brno, Valtická 337, Lednice 69144, Czech Republic. josef.balik@mendelu.cz.
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$a Horník, Štěpán $u Institute of Chemical Process Fundamentals, Academy of Sciences of the Czech Republic, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech Republic. hornik@icpf.cas.cz.
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$a Cuřínová, Petra $u Institute of Chemical Process Fundamentals, Academy of Sciences of the Czech Republic, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech Republic. curinova@icpf.cas.cz.
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$a Sýkora, Jan $u Institute of Chemical Process Fundamentals, Academy of Sciences of the Czech Republic, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech Republic. sykora@icpf.cas.cz.
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