• Je něco špatně v tomto záznamu ?

Multiplex immunoassays for quantification of cytokines, growth factors, and other proteins in stem cell communication

I. Valekova, HK. Skalnikova, K. Jarkovska, J. Motlik, H. Kovarova,

. 2015 ; 1212 (-) : 39-63.

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16010764

Immunoassays represent valuable and broadly used techniques for detection and quantification of proteins. Thanks to their high sensitivity, such techniques are powerful for analyzing growth factors, trophic factors, angiogenic factors, hormones, cytokines, chemokines, soluble receptors, and other proteins which play key roles in intercellular communication and operate as potent regulators of stem cell survival, proliferation, differentiation, or cell death. Multiplex immunological assays, in contrast to ELISA, offer simultaneous quantification of tens of proteins across multiple samples, and have been developed to save time, costs, and sample volumes. Among them, planar antibody microarrays and xMAP(®) bead-based assays have become particularly popular for characterization of proteins secreted by stem cells, as they are relatively easy, highly accurate, multiplex to a high degree and a broad spectrum of analytes can be measured. Here, we describe protocols for multiplex quantification of secreted proteins using Quantibody(®) microarrays (RayBiotech) and xMAP(®) assays (Luminex and its partners).

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16010764
003      
CZ-PrNML
005      
20160415111759.0
007      
ta
008      
160408s2015 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1007/7651_2014_94 $2 doi
024    7_
$a 10.1007/7651_2014_94 $2 doi
035    __
$a (PubMed)25063502
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Valekova, Ivona $u Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Rumburska 89, 27721, Libechov, Czech Republic.
245    10
$a Multiplex immunoassays for quantification of cytokines, growth factors, and other proteins in stem cell communication / $c I. Valekova, HK. Skalnikova, K. Jarkovska, J. Motlik, H. Kovarova,
520    9_
$a Immunoassays represent valuable and broadly used techniques for detection and quantification of proteins. Thanks to their high sensitivity, such techniques are powerful for analyzing growth factors, trophic factors, angiogenic factors, hormones, cytokines, chemokines, soluble receptors, and other proteins which play key roles in intercellular communication and operate as potent regulators of stem cell survival, proliferation, differentiation, or cell death. Multiplex immunological assays, in contrast to ELISA, offer simultaneous quantification of tens of proteins across multiple samples, and have been developed to save time, costs, and sample volumes. Among them, planar antibody microarrays and xMAP(®) bead-based assays have become particularly popular for characterization of proteins secreted by stem cells, as they are relatively easy, highly accurate, multiplex to a high degree and a broad spectrum of analytes can be measured. Here, we describe protocols for multiplex quantification of secreted proteins using Quantibody(®) microarrays (RayBiotech) and xMAP(®) assays (Luminex and its partners).
650    12
$a mezibuněčná komunikace $7 D002450
650    _2
$a buněčné kultury $7 D018929
650    _2
$a kultivované buňky $7 D002478
650    _2
$a kultivační média speciální $x metabolismus $7 D017077
650    _2
$a cytokiny $x metabolismus $7 D016207
650    _2
$a imunoanalýza $x metody $7 D007118
650    _2
$a mezibuněčné signální peptidy a proteiny $x metabolismus $7 D036341
650    _2
$a čipová analýza proteinů $x metody $7 D040081
650    _2
$a kmenové buňky $x fyziologie $7 D013234
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Skalnikova, Helena Kupcova
700    1_
$a Jarkovska, Karla
700    1_
$a Motlik, Jan
700    1_
$a Kovarova, Hana
773    0_
$w MED00180389 $t Methods in molecular biology (Clifton, N.J.) $x 1940-6029 $g Roč. 1212, č. - (2015), s. 39-63
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25063502 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160408 $b ABA008
991    __
$a 20160415111844 $b ABA008
999    __
$a ok $b bmc $g 1114193 $s 935132
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 1212 $c - $d 39-63 $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
LZP    __
$a Pubmed-20160408

Najít záznam

Citační ukazatele

Možnosti archivace