Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

HI-6 treatment does not reactivate sarin inhibited acetylcholinesterase activity in dog brain when administered in human therapeutical dose 30 minutes after the poisoning

Filip Caisberger, Ladislav Novotny, Petr hajek, Jan Misik, Jiri Kassa, Jaroslav Pejchal

. 2016 ; 85 (1) : 2-7.

Jazyk angličtina Země Česko

Typ dokumentu práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16014764

Purpose: The aim of our study was to determine and compare the activity of acetylcholinesterase (AChE) in different parts of dog brain after the exposure to nerve agent sarin with or without HI-6 oxime treatment. Material and methods: Before intoxication, beagle dogs were intravenously anaesthetized and premedicated with atropine sulphate (0.01 mg/kg). Three experimental groups were established – control, sarin (0.03 mg/kg, intramuscularly, 5 min after anaesthesia onset), and sarin + HI-6 dichloride (11.4 mg/kg, intramuscularly, 30 min after sarin poisoning). Brain (amygdaloid body, head of caudate nucleus, somatosensory cortex, Amon’s horn of hippocampus, hypothalamus, brain stem ventral respiratory group, and medial nuclei of thalamus) samples were taken 4 h after sarin administration. AChE activity was detected by histochemistry using the Karnovsky-Roots method and computer image analysis. Results: Sarin poisoning decreased AChE activity in all selected brain areas. HI-6 did not affect this outcome. Conclusion: HI-6 does not reactivate brain AChE in dogs when administered 30 min after sarin poisoning.

Citace poskytuje Crossref.org

Bibliografie atd.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc16014764
003      
CZ-PrNML
005      
20161009135229.0
007      
ta
008      
160518s2016 xr a f 000 0|eng||
009      
AR
024    7_
$a 10.31482/mmsl.2016.001 $2 doi
040    __
$a ABA008 $d ABA008 $e AACR2 $b cze
041    0_
$a eng
044    __
$a xr
100    1_
$a Caisberger, Filip $7 xx0238434 $u Department of Neurology, University Hospital, Hradec Kralove, Czech Republic
245    10
$a HI-6 treatment does not reactivate sarin inhibited acetylcholinesterase activity in dog brain when administered in human therapeutical dose 30 minutes after the poisoning / $c Filip Caisberger, Ladislav Novotny, Petr hajek, Jan Misik, Jiri Kassa, Jaroslav Pejchal
504    __
$a Literatura
520    9_
$a Purpose: The aim of our study was to determine and compare the activity of acetylcholinesterase (AChE) in different parts of dog brain after the exposure to nerve agent sarin with or without HI-6 oxime treatment. Material and methods: Before intoxication, beagle dogs were intravenously anaesthetized and premedicated with atropine sulphate (0.01 mg/kg). Three experimental groups were established – control, sarin (0.03 mg/kg, intramuscularly, 5 min after anaesthesia onset), and sarin + HI-6 dichloride (11.4 mg/kg, intramuscularly, 30 min after sarin poisoning). Brain (amygdaloid body, head of caudate nucleus, somatosensory cortex, Amon’s horn of hippocampus, hypothalamus, brain stem ventral respiratory group, and medial nuclei of thalamus) samples were taken 4 h after sarin administration. AChE activity was detected by histochemistry using the Karnovsky-Roots method and computer image analysis. Results: Sarin poisoning decreased AChE activity in all selected brain areas. HI-6 did not affect this outcome. Conclusion: HI-6 does not reactivate brain AChE in dogs when administered 30 min after sarin poisoning.
650    12
$a sarin $x otrava $7 D012524
650    _2
$a cholinesterasové inhibitory $x otrava $7 D002800
650    12
$a antidota $x farmakologie $7 D000931
650    12
$a mozek $x enzymologie $x patologie $7 D001921
650    _2
$a histologické techniky $7 D006652
650    _2
$a pralidoximové sloučeniny $x farmakologie $x terapeutické užití $7 D011220
650    _2
$a pyridinové sloučeniny $x farmakologie $x terapeutické užití $7 D011726
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a modely nemocí na zvířatech $7 D004195
650    _2
$a zvířata $7 D000818
650    _2
$a psi $7 D004285
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Novotný, Ladislav $7 _AN063631 $u Department of Histology and Embryology, Medical Faculty of Charles University, Hradec Kralove, Czech Republic
700    1_
$a Hajek, Petr $u Deparmtnet of Anatomy, Medical Faculty of Charles University, Hradec Kralove, Czech Republic
700    1_
$a Misík, Jan $7 xx0234998 $u Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec kralove, Czech Republic
700    1_
$a Kassa, Jiří, $d 1956- $7 mzk2003181395 $u Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec kralove, Czech Republic
700    1_
$a Pejchal, Jaroslav $7 xx0102560 $u Department of Radiobiolgy, Faculty of Military Health Sciences, University of Defence, Hradec kralove, Czech Republic
773    0_
$t Vojenské zdravotnické listy $x 0372-7025 $g Roč. 85, č. 1 (2016), s. 2-7 $w MED00011116
856    41
$u http://www.mmsl.cz/archive $y domovská stránka časopisu
910    __
$a ABA008 $b A 3 $c 1073 $y 4 $z 0
990    __
$a 20150120115154 $b ABA008
991    __
$a 20161009135546 $b ABA008
999    __
$a ok $b bmc $g 1124943 $s 939184
BAS    __
$a 3
BMC    __
$a 2016 $b 85 $c 1 $d 2-7 $i 0372-7025 $m Vojenské zdravotnické listy $x MED00011116
LZP    __
$c NLK125 $d 20160624 $a NLK 2016-20/dk

Najít záznam

Citační ukazatele

Možnosti archivace