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MALDI-TOF MSI and electrochemical detection of metallothionein in chicken liver after cadmium exposure

Roman Guran, Iva Blazkova, Renata Kensova, Lukas Richtera, Lucie Blazkova, Ondrej Zitka, Rene Kizek, Vojtech Adam

. 2015 ; 2 (3) : 43-49.

Jazyk angličtina Země Česko

Typ dokumentu práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16016327

Metallomics is an area that studies the interactions of metal ions with proteins and other biomolecules and their function in living organisms. Metallothioneins (MTs) belong to a large family of metalloproteins in this field. As it is known, the presence of Cd2+ ions causes higher expression of MTs. Therefore we focused on determination of MT and Cd content in liver from chicken embryos (model organism) by electrochemistry and atomic absorption spectroscopy (AAS) after the exposure of chicken embryos to different concentrations of Cd(NO3)2. We also determined the spatial distribution of MT in chicken liver tissue slices by matrix assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI) and evaluated the toxicity of Cd(NO3)2 to chicken embryos. Finally, we attempted to find the correlation between 2D MSI maps of MT and concentration of MT in chicken liver.

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$a Guráň, Roman $7 _AN082259 $u Department of Chemistry and Biochemistry, Mendel University in Brno,Czech Republic; Central European Institute of Technology, Brno University of Technology, Czech Republic
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$a MALDI-TOF MSI and electrochemical detection of metallothionein in chicken liver after cadmium exposure / $c Roman Guran, Iva Blazkova, Renata Kensova, Lukas Richtera, Lucie Blazkova, Ondrej Zitka, Rene Kizek, Vojtech Adam
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$a Blažková, Iva $7 _AN074413 $u Department of Chemistry and Biochemistry, Mendel University in Brno,Czech Republic; Central European Institute of Technology, Brno University of Technology, Czech Republic
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$a Kenšová, Renata $7 _AN075702 $u Department of Chemistry and Biochemistry, Mendel University in Brno,Czech Republic; Central European Institute of Technology, Brno University of Technology, Czech Republic
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$a Richtera, Lukáš $7 _AN087460 $u Department of Chemistry and Biochemistry, Mendel University in Brno,Czech Republic; Central European Institute of Technology, Brno University of Technology, Czech Republic
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$a Blažková, Lucie $7 _AN087461 $u Department of Chemistry and Biochemistry, Mendel University in Brno,Czech Republic; Central European Institute of Technology, Brno University of Technology, Czech Republic
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$a Zítka, Ondřej $7 xx0116134 $u Department of Chemistry and Biochemistry, Mendel University in Brno,Czech Republic; Central European Institute of Technology, Brno University of Technology, Czech Republic
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$a Kizek, René, $d 1972- $7 jn20001005291 $u Department of Chemistry and Biochemistry, Mendel University in Brno,Czech Republic; Central European Institute of Technology, Brno University of Technology, Czech Republic
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$a Adam, Vojtěch, $d 1982- $7 xx0064599 $u Department of Chemistry and Biochemistry, Mendel University in Brno,Czech Republic; Central European Institute of Technology, Brno University of Technology, Czech Republic
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