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Modification of anti-DNA antibodies with carbon quantum dots

Simona Dostalova, Marketa Vaculovicova, Sona Krizkova, Lukas Richtera, Pavel Kopel, Rene Kizek

. 2015 ; 2 (3) : 102-109.

Jazyk angličtina Země Česko

Typ dokumentu práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16016346

The anti-DNA antibodies are produced in patients with autoimmune disease called systemic lupus erythematodes. They can be reactive against double or single stranded DNA or DNA modified with some other molecules. Using the variety of antibodies it is possible to determine the structure of studied DNA. In this work, we used 4 anti-DNA antibodies produced in egg yolk after immunization of hens with DNA-mBSA antigen – anti-dsDNA, anti-ssDNA, afi-dsDNA and afi-ssDNA. The reactivity of these antibodies was evaluated using the dot blot method with different lengths and concentrations of DNA antigen. The most reactive antibodies (anti-ssDNA) were modified with carbon quantum dots synthesized from multiwall carbon nanotubes and this modification was verified by ELISA-like method with fluorescent detection and fluorescence resonance energy transfer between DNA and quantum dots was observed, increasing the sensitivity of the DNA detection.

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$a Dostálová, Simona $7 _AN082240 $u Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Czech Republic, European Union; Central European Institute of Technology, Brno University of Technology, Czech Republic, European Union
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$a The anti-DNA antibodies are produced in patients with autoimmune disease called systemic lupus erythematodes. They can be reactive against double or single stranded DNA or DNA modified with some other molecules. Using the variety of antibodies it is possible to determine the structure of studied DNA. In this work, we used 4 anti-DNA antibodies produced in egg yolk after immunization of hens with DNA-mBSA antigen – anti-dsDNA, anti-ssDNA, afi-dsDNA and afi-ssDNA. The reactivity of these antibodies was evaluated using the dot blot method with different lengths and concentrations of DNA antigen. The most reactive antibodies (anti-ssDNA) were modified with carbon quantum dots synthesized from multiwall carbon nanotubes and this modification was verified by ELISA-like method with fluorescent detection and fluorescence resonance energy transfer between DNA and quantum dots was observed, increasing the sensitivity of the DNA detection.
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$a Vaculovičová, Markéta $7 _AN082228 $u Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Czech Republic, European Union; Central European Institute of Technology, Brno University of Technology, Czech Republic, European Union
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$a Křížková, Soňa, $d 1981- $7 xx0105812 $u Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Czech Republic, European Union; Central European Institute of Technology, Brno University of Technology, Czech Republic, European Union
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$a Richtera, Lukáš $7 _AN087460 $u Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Czech Republic, European Union; Central European Institute of Technology, Brno University of Technology, Czech Republic, European Union
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$a Kopel, Pavel, $d 1965- $7 mzk2005300913 $u Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Czech Republic, European Union; Central European Institute of Technology, Brno University of Technology, Czech Republic, European Union
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$a Kizek, René, $d 1972- $7 jn20001005291 $u Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Czech Republic, European Union; Central European Institute of Technology, Brno University of Technology, Czech Republic, European Union
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