-
Je něco špatně v tomto záznamu ?
In vivo vascularization of anisotropic channeled porous polylactide-based capsules for islet transplantation: the effects of scaffold architecture and implantation site
N. Kasoju, D. Kubies, E. Fábryová, J. Kříž, M. M. Kumorek, E. Sticová, F. Rypáček
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NT14240
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
Zdroj
NLK
Directory of Open Access Journals
od 1991
Free Medical Journals
od 1998
ProQuest Central
od 2005-01-01
Medline Complete (EBSCOhost)
od 2006-01-01
Nursing & Allied Health Database (ProQuest)
od 2005-01-01
Health & Medicine (ProQuest)
od 2005-01-01
ROAD: Directory of Open Access Scholarly Resources
od 1998
- MeSH
- anizotropie MeSH
- antigeny CD31 MeSH
- buněčné mikroprostředí MeSH
- fibróza MeSH
- fyziologická neovaskularizace účinky léků MeSH
- krysa rodu rattus MeSH
- kyselina mléčná chemie MeSH
- kyselina polyglykolová chemie MeSH
- Langerhansovy ostrůvky * MeSH
- poréznost MeSH
- potkani inbrední BN MeSH
- tkáňové podpůrné struktury MeSH
- tobolky MeSH
- transplantace Langerhansových ostrůvků metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The replacement of pancreatic islets for the possible treatment of type 1 diabetes is limited by the extremely high oxygen demand of the islets. To this end, here we hypothesize to create a novel extra-hepatic highly-vascularized bioartificial cavity using a porous scaffold as a template and using the host body as a living bioreactor for subsequent islet transplantation. Polylactide-based capsular-shaped anisotropic channeled porous scaffolds were prepared by following the unidirectional thermally-induced phase separation technique, and were implanted under the skin and in the greater omentum of Brown Norway rats. Polyamide mesh-based isotropic regular porous capsules were used as the controls. After 4weeks, the implants were excised and analyzed by histology. The hematoxylin and eosin, as well as Masson's trichrome staining, revealed a) low or no infiltration of giant inflammatory cells in the implant, b) minor but insignificant fibrosis around the implant, c) guided infiltration of host cells in the test capsule in contrast to random cell infiltration in the control capsule, and d) relatively superior cell infiltration in the capsules implanted in the greater omentum than in the capsules implanted under the skin. Furthermore, the anti-CD31 immunohistochemistry staining revealed numerous vessels at the implant site, but mostly on the external surface of the capsules. Taken together, the current study, the first of its kind, is a significant step-forward towards engineering a bioartificial microenvironment for the transplantation of islets.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16019731
- 003
- CZ-PrNML
- 005
- 20191017093342.0
- 007
- ta
- 008
- 160721s2015 xr a f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.33549/physiolres.933138 $2 doi
- 035 __
- $a (PubMed)26447597
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Kasoju, N. $u Department of Biomaterials and Bioanalogous Polymer Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic $7 _AN088008
- 245 10
- $a In vivo vascularization of anisotropic channeled porous polylactide-based capsules for islet transplantation: the effects of scaffold architecture and implantation site / $c N. Kasoju, D. Kubies, E. Fábryová, J. Kříž, M. M. Kumorek, E. Sticová, F. Rypáček
- 520 9_
- $a The replacement of pancreatic islets for the possible treatment of type 1 diabetes is limited by the extremely high oxygen demand of the islets. To this end, here we hypothesize to create a novel extra-hepatic highly-vascularized bioartificial cavity using a porous scaffold as a template and using the host body as a living bioreactor for subsequent islet transplantation. Polylactide-based capsular-shaped anisotropic channeled porous scaffolds were prepared by following the unidirectional thermally-induced phase separation technique, and were implanted under the skin and in the greater omentum of Brown Norway rats. Polyamide mesh-based isotropic regular porous capsules were used as the controls. After 4weeks, the implants were excised and analyzed by histology. The hematoxylin and eosin, as well as Masson's trichrome staining, revealed a) low or no infiltration of giant inflammatory cells in the implant, b) minor but insignificant fibrosis around the implant, c) guided infiltration of host cells in the test capsule in contrast to random cell infiltration in the control capsule, and d) relatively superior cell infiltration in the capsules implanted in the greater omentum than in the capsules implanted under the skin. Furthermore, the anti-CD31 immunohistochemistry staining revealed numerous vessels at the implant site, but mostly on the external surface of the capsules. Taken together, the current study, the first of its kind, is a significant step-forward towards engineering a bioartificial microenvironment for the transplantation of islets.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a anizotropie $7 D016880
- 650 _2
- $a antigeny CD31 $7 D019408
- 650 _2
- $a tobolky $7 D002214
- 650 _2
- $a buněčné mikroprostředí $7 D060833
- 650 _2
- $a fibróza $7 D005355
- 650 12
- $a Langerhansovy ostrůvky $7 D007515
- 650 _2
- $a transplantace Langerhansových ostrůvků $x metody $7 D016381
- 650 _2
- $a kyselina mléčná $x chemie $7 D019344
- 650 _2
- $a fyziologická neovaskularizace $x účinky léků $7 D018919
- 650 _2
- $a kyselina polyglykolová $x chemie $7 D011100
- 650 _2
- $a poréznost $7 D016062
- 650 _2
- $a krysa rodu Rattus $7 D051381
- 650 _2
- $a potkani inbrední BN $7 D011914
- 650 _2
- $a tkáňové podpůrné struktury $7 D054457
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Kubies, Dana $u Department of Biomaterials and Bioanalogous Polymer Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic $7 xx0114169
- 700 1_
- $a Fábryová, Eva $7 _AN074825 $u Center of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
- 700 1_
- $a Kříž, Jan, $d 1972- $7 xx0056572 $u Center of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic; Department of Diabetes, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
- 700 1_
- $a Kumorek, M. M. $d Department of Biomaterials and Bioanalogous Polymer Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic $7 _AN088009
- 700 1_
- $a Sticová, Eva $7 xx0108073 $u Department of Pathology, Third Faculty of Medicine, Charles University and Faculty Hospital of Kralovske Vinohrady, Prague, Czech Republic
- 700 1_
- $a Rypáček, František, $d 1947- $7 jk01103352 $u Department of Biomaterials and Bioanalogous Polymer Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
- 773 0_
- $w MED00003824 $t Physiological research Academia Scientiarum Bohemoslovaca $x 1802-9973 $g Roč. 64, Suppl. 1 (2015), s. S75-S84
- 773 0_
- $t Recent advances in BIOCEV research projects $g (2015), s. S75-S84 $w MED00191407
- 856 41
- $u http://www.biomed.cas.cz/physiolres/ $y domovská stránka časopisu
- 910 __
- $a ABA008 $b A 4120 $c 266 $y 4 $z 0
- 990 __
- $a 20160721 $b ABA008
- 991 __
- $a 20191017093810 $b ABA008
- 999 __
- $a ok $b bmc $g 1156628 $s 944259
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 64 $c Suppl. 1 $d S75-S84 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
- BMC __
- $a 2015 $d S75-S84 $m Recent advances in BIOCEV research projects $x MED00191407
- GRA __
- $a NT14240 $p MZ0
- LZP __
- $b NLK118 $a Pubmed-20160721
