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Using dot blot with immunochemical detection to evaluate global changes in SUMO-2/3 conjugation
M. Častorálová, T. Ruml, Z. Knejzlík,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Freely Accessible Science Journals
from 1996
Taylor & Francis Open Access
from 1996-01-01
PubMed
26307261
DOI
10.2144/000113925
Knihovny.cz E-resources
- MeSH
- HEK293 Cells MeSH
- Immunoblotting methods MeSH
- Humans MeSH
- Small Ubiquitin-Related Modifier Proteins * analysis chemistry metabolism MeSH
- Ubiquitins * analysis chemistry metabolism MeSH
- Blotting, Western MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Small ubiquitin-related modifier-2/3 (SUMO-2/3) is a member of the ubiquitin-like (Ubl) protein family. Conjugation of SUMO-2/3 to target proteins is influenced by various stress conditions and chemical inhibitors. SUMO-2/3 conjugation may serve as a neuroprotective mechanism and may play a role in protein quality control. A method for screening global changes in SUMO-2/3 conjugation would facilitate further research of SUMO-2/3 cellular function. Here we show that dot blot with immunochemical detection allows evaluation of changes in global cellular SUMO-2/3 conjugation and offers an alternative to more laborious Western blot analysis. The method is based on a change of SUMO-2/3 signal intensity upon its conjugation. The dot blot analysis presented here is a time-saving method that enables screening of large numbers of samples and easy statistical evaluation of the results.
References provided by Crossref.org
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