-
Something wrong with this record ?
DNA-Based Taxonomy in Ecologically Versatile Microalgae: A Re-Evaluation of the Species Concept within the Coccoid Green Algal Genus Coccomyxa (Trebouxiophyceae, Chlorophyta)
V. Malavasi, P. Škaloud, F. Rindi, S. Tempesta, M. Paoletti, M. Pasqualetti,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Directory of Open Access Journals
from 2006
Free Medical Journals
from 2006
Public Library of Science (PLoS)
from 2006
PubMed Central
from 2006
Europe PubMed Central
from 2006
ProQuest Central
from 2006-12-01
Open Access Digital Library
from 2006-10-01
Open Access Digital Library
from 2006-01-01
Open Access Digital Library
from 2006-01-01
Medline Complete (EBSCOhost)
from 2008-01-01
Nursing & Allied Health Database (ProQuest)
from 2006-12-01
Health & Medicine (ProQuest)
from 2006-12-01
Public Health Database (ProQuest)
from 2006-12-01
ROAD: Directory of Open Access Scholarly Resources
from 2006
- MeSH
- Chlorophyta classification genetics ultrastructure MeSH
- DNA, Plant genetics MeSH
- Phylogeny MeSH
- DNA, Ribosomal Spacer genetics MeSH
- Microalgae classification genetics ultrastructure MeSH
- Evolution, Molecular MeSH
- Sequence Analysis, DNA MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Coccomyxa is a genus of unicellular green algae of the class Trebouxiophyceae, well known for its cosmopolitan distribution and great ecological amplitude. The taxonomy of this genus has long been problematic, due to reliance on badly-defined and environmentally variable morphological characters. In this study, based on the discovery of a new species from an extreme habitat, we reassess species circumscription in Coccomyxa, a unicellular genus of the class Trebouxiophyceae, using a combination of ecological and DNA sequence data (analyzed with three different methods of algorithmic species delineation). Our results are compared with those of a recent integrative study of Darienko and colleagues that reassessed the taxonomy of Coccomyxa, recognizing 7 species in the genus. Expanding the dataset from 43 to 61 sequences (SSU + ITS rDNA) resulted in a different delimitation, supporting the recognition of a higher number of species (24 to 27 depending on the analysis used, with the 27-species scenario receiving the strongest support). Among these, C. melkonianii sp. nov. is described from material isolated from a river highly polluted by heavy metals (Rio Irvi, Sardinia, Italy). Analyses performed on ecological characters detected a significant phylogenetic signal in six different characters. We conclude that the 27-species scenario is presently the most realistic for Coccomyxa and we suggest that well-supported lineages distinguishable by ecological preferences should be recognized as different species in this genus. We also recommend that for microbial lineages in which the overall diversity is unknown and taxon sampling is sparse, as is often the case for green microalgae, the results of analyses for algorithmic DNA-based species delimitation should be interpreted with extreme caution.
Department of biological and ecological sciences Tuscia University Viterbo Italy
Department of Botany Faculty of Science Charles University of Prague Prague Czech Republic
Dipartimento di Scienze della Vita e dell'Ambiente Università Politecnica delle Marche Ancona Italy
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16027564
- 003
- CZ-PrNML
- 005
- 20161005132146.0
- 007
- ta
- 008
- 161005s2016 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1371/journal.pone.0151137 $2 doi
- 024 7_
- $a 10.1371/journal.pone.0151137 $2 doi
- 035 __
- $a (PubMed)27028195
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Malavasi, Veronica $u Interdepartmental Center of Environmental Science and Engineering (CINSA), University of Cagliari, Cagliari, Italy.
- 245 10
- $a DNA-Based Taxonomy in Ecologically Versatile Microalgae: A Re-Evaluation of the Species Concept within the Coccoid Green Algal Genus Coccomyxa (Trebouxiophyceae, Chlorophyta) / $c V. Malavasi, P. Škaloud, F. Rindi, S. Tempesta, M. Paoletti, M. Pasqualetti,
- 520 9_
- $a Coccomyxa is a genus of unicellular green algae of the class Trebouxiophyceae, well known for its cosmopolitan distribution and great ecological amplitude. The taxonomy of this genus has long been problematic, due to reliance on badly-defined and environmentally variable morphological characters. In this study, based on the discovery of a new species from an extreme habitat, we reassess species circumscription in Coccomyxa, a unicellular genus of the class Trebouxiophyceae, using a combination of ecological and DNA sequence data (analyzed with three different methods of algorithmic species delineation). Our results are compared with those of a recent integrative study of Darienko and colleagues that reassessed the taxonomy of Coccomyxa, recognizing 7 species in the genus. Expanding the dataset from 43 to 61 sequences (SSU + ITS rDNA) resulted in a different delimitation, supporting the recognition of a higher number of species (24 to 27 depending on the analysis used, with the 27-species scenario receiving the strongest support). Among these, C. melkonianii sp. nov. is described from material isolated from a river highly polluted by heavy metals (Rio Irvi, Sardinia, Italy). Analyses performed on ecological characters detected a significant phylogenetic signal in six different characters. We conclude that the 27-species scenario is presently the most realistic for Coccomyxa and we suggest that well-supported lineages distinguishable by ecological preferences should be recognized as different species in this genus. We also recommend that for microbial lineages in which the overall diversity is unknown and taxon sampling is sparse, as is often the case for green microalgae, the results of analyses for algorithmic DNA-based species delimitation should be interpreted with extreme caution.
- 650 _2
- $a Chlorophyta $x klasifikace $x genetika $x ultrastruktura $7 D000460
- 650 _2
- $a DNA rostlinná $x genetika $7 D018744
- 650 _2
- $a mezerníky ribozomální DNA $x genetika $7 D021903
- 650 _2
- $a molekulární evoluce $7 D019143
- 650 _2
- $a mikrořasy $x klasifikace $x genetika $x ultrastruktura $7 D058086
- 650 _2
- $a fylogeneze $7 D010802
- 650 _2
- $a sekvenční analýza DNA $7 D017422
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Škaloud, Pavel $u Department of Botany, Faculty of Science, Charles University of Prague, Prague, Czech Republic.
- 700 1_
- $a Rindi, Fabio $u Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Ancona, Italy.
- 700 1_
- $a Tempesta, Sabrina $u Department of biological and ecological sciences, Tuscia University, Viterbo, Italy.
- 700 1_
- $a Paoletti, Michela $u Department of biological and ecological sciences, Tuscia University, Viterbo, Italy.
- 700 1_
- $a Pasqualetti, Marcella $u Department of biological and ecological sciences, Tuscia University, Viterbo, Italy.
- 773 0_
- $w MED00180950 $t PloS one $x 1932-6203 $g Roč. 11, č. 3 (2016), s. e0151137
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/27028195 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20161005 $b ABA008
- 991 __
- $a 20161005132533 $b ABA008
- 999 __
- $a ok $b bmc $g 1165878 $s 952194
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 11 $c 3 $d e0151137 $e 20160330 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
- LZP __
- $a Pubmed-20161005
