• Je něco špatně v tomto záznamu ?

The Biological Effects of Bilirubin Photoisomers

J. Jasprova, M. Dal Ben, E. Vianello, I. Goncharova, M. Urbanova, K. Vyroubalova, S. Gazzin, C. Tiribelli, M. Sticha, M. Cerna, L. Vitek,

. 2016 ; 11 (2) : e0148126. [pub] 20160201

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16027702

Although phototherapy was introduced as early as 1950's, the potential biological effects of bilirubin photoisomers (PI) generated during phototherapy remain unclear. The aim of our study was to isolate bilirubin PI in their pure forms and to assess their biological effects in vitro. The three major bilirubin PI (ZE- and EZ-bilirubin and Z-lumirubin) were prepared by photo-irradiation of unconjugated bilirubin. The individual photoproducts were chromatographically separated (TLC, HPLC), and their identities verified by mass spectrometry. The role of Z-lumirubin (the principle bilirubin PI) on the dissociation of bilirubin from albumin was tested by several methods: peroxidase, fluorescence quenching, and circular dichroism. The biological effects of major bilirubin PI (cell viability, expression of selected genes, cell cycle progression) were tested on the SH-SY5Y human neuroblastoma cell line. Lumirubin was found to have a binding site on human serum albumin, in the subdomain IB (or at a close distance to it); and thus, different from that of bilirubin. Its binding constant to albumin was much lower when compared with bilirubin, and lumirubin did not affect the level of unbound bilirubin (Bf). Compared to unconjugated bilirubin, bilirubin PI did not have any effect on either SH-SY5Y cell viability, the expression of genes involved in bilirubin metabolism or cell cycle progression, nor in modulation of the cell cycle phase. The principle bilirubin PI do not interfere with bilirubin albumin binding, and do not exert any toxic effect on human neuroblastoma cells.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16027702
003      
CZ-PrNML
005      
20161005132258.0
007      
ta
008      
161005s2016 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1371/journal.pone.0148126 $2 doi
024    7_
$a 10.1371/journal.pone.0148126 $2 doi
035    __
$a (PubMed)26829016
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Jasprova, Jana $u Institute of Medical Biochemistry and Laboratory Diagnostics, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
245    14
$a The Biological Effects of Bilirubin Photoisomers / $c J. Jasprova, M. Dal Ben, E. Vianello, I. Goncharova, M. Urbanova, K. Vyroubalova, S. Gazzin, C. Tiribelli, M. Sticha, M. Cerna, L. Vitek,
520    9_
$a Although phototherapy was introduced as early as 1950's, the potential biological effects of bilirubin photoisomers (PI) generated during phototherapy remain unclear. The aim of our study was to isolate bilirubin PI in their pure forms and to assess their biological effects in vitro. The three major bilirubin PI (ZE- and EZ-bilirubin and Z-lumirubin) were prepared by photo-irradiation of unconjugated bilirubin. The individual photoproducts were chromatographically separated (TLC, HPLC), and their identities verified by mass spectrometry. The role of Z-lumirubin (the principle bilirubin PI) on the dissociation of bilirubin from albumin was tested by several methods: peroxidase, fluorescence quenching, and circular dichroism. The biological effects of major bilirubin PI (cell viability, expression of selected genes, cell cycle progression) were tested on the SH-SY5Y human neuroblastoma cell line. Lumirubin was found to have a binding site on human serum albumin, in the subdomain IB (or at a close distance to it); and thus, different from that of bilirubin. Its binding constant to albumin was much lower when compared with bilirubin, and lumirubin did not affect the level of unbound bilirubin (Bf). Compared to unconjugated bilirubin, bilirubin PI did not have any effect on either SH-SY5Y cell viability, the expression of genes involved in bilirubin metabolism or cell cycle progression, nor in modulation of the cell cycle phase. The principle bilirubin PI do not interfere with bilirubin albumin binding, and do not exert any toxic effect on human neuroblastoma cells.
650    _2
$a bilirubin $x chemie $x izolace a purifikace $x farmakologie $7 D001663
650    _2
$a buněčný cyklus $x účinky léků $7 D002453
650    _2
$a buněčné linie $7 D002460
650    _2
$a viabilita buněk $x účinky léků $7 D002470
650    _2
$a vysokoúčinná kapalinová chromatografie $7 D002851
650    _2
$a cirkulární dichroismus $7 D002942
650    _2
$a regulace genové exprese $x účinky léků $7 D005786
650    _2
$a hem $x metabolismus $7 D006418
650    _2
$a lidé $7 D006801
650    _2
$a isomerie $7 D007536
650    _2
$a kinetika $7 D007700
650    _2
$a ligandy $7 D008024
650    12
$a světlo $7 D008027
650    _2
$a fototerapie $7 D010789
650    _2
$a sérový albumin $x metabolismus $7 D012709
650    _2
$a spektrofotometrie ultrafialová $7 D013056
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Dal Ben, Matteo $u Italian Liver Foundation, CSF, Trieste, Italy.
700    1_
$a Vianello, Eleonora $u Italian Liver Foundation, CSF, Trieste, Italy.
700    1_
$a Goncharova, Iryna $u Institute of Chemical Technology Prague, Prague, Czech Republic.
700    1_
$a Urbanova, Marie $u Institute of Chemical Technology Prague, Prague, Czech Republic.
700    1_
$a Vyroubalova, Karolina $u Institute of Medical Biochemistry and Laboratory Diagnostics, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
700    1_
$a Gazzin, Silvia $u Italian Liver Foundation, CSF, Trieste, Italy.
700    1_
$a Tiribelli, Claudio $u Italian Liver Foundation, CSF, Trieste, Italy.
700    1_
$a Sticha, Martin $u Faculty of Science, Charles University in Prague, Prague, Czech Republic.
700    1_
$a Cerna, Marcela $u The Institute for Mother and Child, Prague, Czech Republic.
700    1_
$a Vitek, Libor $u Institute of Medical Biochemistry and Laboratory Diagnostics, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. 4th Department of Internal Medicine, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
773    0_
$w MED00180950 $t PloS one $x 1932-6203 $g Roč. 11, č. 2 (2016), s. e0148126
856    41
$u https://pubmed.ncbi.nlm.nih.gov/26829016 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20161005 $b ABA008
991    __
$a 20161005132645 $b ABA008
999    __
$a ok $b bmc $g 1166016 $s 952332
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2016 $b 11 $c 2 $d e0148126 $e 20160201 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
LZP    __
$a Pubmed-20161005

Najít záznam

Citační ukazatele

Možnosti archivace