Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

DDE downregulates PLIN2 expression during differentiation of mesenchymal stem cells into adipocytes in lipid-enriched medium

Dana Müllerová, Martin Pešta, Miroslava Čedíková, Jana Dvořáková, Vlastimil Kulda, Kristýna Srbecká, Luděk Müller, Pavel Dvořák, Michaela Kripnerová, Milena Králíčková, Václav Babuška, Jitka Kuncová

. 2016 ; 14 (2) : 113-117.

Jazyk angličtina Země Česko

Typ dokumentu práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16029026

Grantová podpora
NT14330 MZ0 CEP - Centrální evidence projektů

Evidence indicating, that persistent organic pollutants are involved in the development of obesity, has emerged. The aim of this study was to reveal whether an environmental bioaccumulative human adipose tissue contaminant, 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE), affects adipocyte differentiation. Our study was conducted on an in vitro adipogenic model of human adipose derived mesenchymal stem cells (hADMSC). The adipose cultures were exposed to DDE (concentrations: 0.1 μM, 1 μM, and 10 μM) for 28 consecutive days, from the beginning of the experiment until full differentiation. DDE was administered in lipid vehicle (NuTRIflex). Samples for gene expression analysis by RT real-time PCR were collected on days 0, 4, 10, 21 and 28 during the course of differentiation. Differentiating adipocytes cultivated in lipid-rich medium (NuTRIflex) increased the expression of perilipin 2 (PLIN2). However, the addition of DDE suppressed this effect (p < 0.03). Our results may suggest that upregulation of PLIN2, caused by exposure to lipids during the differentiation of adipocytes, is reduced in the presence of DDE. This effect of DDE warrants future attention, because of the important role of PLIN2 in formation and stabilization of lipid droplets, as the impairment of their function could be linked to the worldwide obesity epidemic.

Citace poskytuje Crossref.org

Bibliografie atd.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc16029026
003      
CZ-PrNML
005      
20191018091841.0
007      
ta
008      
161009s2016 xr ad f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.jab.2016.01.002 $2 doi
040    __
$a ABA008 $d ABA008 $e AACR2 $b cze
041    0_
$a eng
044    __
$a xr
100    1_
$a Müllerová, Dana, $d 1962- $7 xx0011940 $u Department of Public Health and Preventive Medicine, Faculty of Medicine in Pilsen, Charles University in Prague, Lidická 4, Plzeň 301 66, Czech Republic
245    10
$a DDE downregulates PLIN2 expression during differentiation of mesenchymal stem cells into adipocytes in lipid-enriched medium / $c Dana Müllerová, Martin Pešta, Miroslava Čedíková, Jana Dvořáková, Vlastimil Kulda, Kristýna Srbecká, Luděk Müller, Pavel Dvořák, Michaela Kripnerová, Milena Králíčková, Václav Babuška, Jitka Kuncová
504    __
$a Literatura
520    9_
$a Evidence indicating, that persistent organic pollutants are involved in the development of obesity, has emerged. The aim of this study was to reveal whether an environmental bioaccumulative human adipose tissue contaminant, 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE), affects adipocyte differentiation. Our study was conducted on an in vitro adipogenic model of human adipose derived mesenchymal stem cells (hADMSC). The adipose cultures were exposed to DDE (concentrations: 0.1 μM, 1 μM, and 10 μM) for 28 consecutive days, from the beginning of the experiment until full differentiation. DDE was administered in lipid vehicle (NuTRIflex). Samples for gene expression analysis by RT real-time PCR were collected on days 0, 4, 10, 21 and 28 during the course of differentiation. Differentiating adipocytes cultivated in lipid-rich medium (NuTRIflex) increased the expression of perilipin 2 (PLIN2). However, the addition of DDE suppressed this effect (p < 0.03). Our results may suggest that upregulation of PLIN2, caused by exposure to lipids during the differentiation of adipocytes, is reduced in the presence of DDE. This effect of DDE warrants future attention, because of the important role of PLIN2 in formation and stabilization of lipid droplets, as the impairment of their function could be linked to the worldwide obesity epidemic.
650    _2
$a kultivované buňky $7 D002478
650    12
$a dichlordifenyldichlorethylen $7 D003633
650    _2
$a pesticidy $7 D010575
650    12
$a membránové proteiny $x genetika $7 D008565
650    _2
$a buněčná diferenciace $x účinky léků $7 D002454
650    12
$a tukové buňky $x metabolismus $x účinky léků $7 D017667
650    _2
$a lipidová tělíska $x metabolismus $7 D066292
650    _2
$a upregulace $7 D015854
650    _2
$a mezenchymální kmenové buňky $7 D059630
650    _2
$a neparametrická statistika $7 D018709
650    _2
$a látky znečišťující životní prostředí $x metabolismus $7 D004785
653    00
$a perilipin-2
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Pešta, Martin, $d 1976- $7 xx0073915 $u Department of Biology, Faculty of Medicine in Pilsen, Charles University in Prague, alej Svobody 76, Plzeň 323 00, Czech Republic; Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, alej Svobody 76, Plzeň 323 00, Czech Republic
700    1_
$a Čedíková, Miroslava $7 xx0099423 $u Department of Physiology, Faculty of Medicine in Pilsen, Charles University in Prague, alej Svobody 76, Plzeň 323 00, Czech Republic; Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, alej Svobody 76, Plzeň 323 00, Czech Republic
700    1_
$a Dvořáková, Jana $7 _AN070612 $u Department of Public Health and Preventive Medicine, Faculty of Medicine in Pilsen, Charles University in Prague, Lidická 4, Plzeň 301 66, Czech Republic
700    1_
$a Kulda, Vlastimil, $d 1979- $7 xx0138688
700    1_
$a Srbecká, Kristýna $7 xx0218151 $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine in Pilsen, Charles University in Prague, Karlovarská 48, Plzeň 301 66, Czech Republic
700    1_
$a Müller, Luděk, $d 1961- $7 stk2007383203 $u Department of Cybernetics, Faculty of Applied Sciences, University of West Bohemia, Univerzitní 8, Plzeň 306 14, Czech Republic
700    1_
$a Dvořák, Pavel $7 _AN047353 $u Department of Biology, Faculty of Medicine in Pilsen, Charles University in Prague, alej Svobody 76, Plzeň 323 00, Czech Republic
700    1_
$a Kripnerová, Michaela $7 xx0232916 $u Department of Biology, Faculty of Medicine in Pilsen, Charles University in Prague, alej Svobody 76, Plzeň 323 00, Czech Republic
700    1_
$a Králíčková, Milena, $d 1972- $7 xx0069996 $u Department of Histology, Faculty of Medicine in Pilsen, Charles University in Prague, Karlovarská 48, Plzeň 301 66, Czech Republic; Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, alej Svobody 76, Plzeň 323 00, Czech Republic
700    1_
$a Babuška, Václav, $d 1971- $7 xx0138687 $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine in Pilsen, Charles University in Prague, Karlovarská 48, Plzeň 301 66, Czech Republic
700    1_
$a Kuncová, Jitka, $d 1964- $7 xx0078884 $u Department of Physiology, Faculty of Medicine in Pilsen, Charles University in Prague, alej Svobody 76, Plzeň 323 00, Czech Republic; Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, alej Svobody 76, Plzeň 323 00, Czech Republic
773    0_
$t Journal of applied biomedicine $x 1214-021X $g Roč. 14, č. 2 (2016), s. 113-117 $w MED00012667
856    41
$u https://jab.zsf.jcu.cz/pdfs/jab/2016/02/05.pdf $y plný text volně přístupný
910    __
$a ABA008 $b B 2301 $c 1249 $y 4 $z 0
990    __
$a 20150522215938 $b ABA008
991    __
$a 20191018092311 $b ABA008
999    __
$a ok $b bmc $g 1167503 $s 953661
BAS    __
$a 3
BMC    __
$a 2016 $b 14 $c 2 $d 113-117 $i 1214-021X $m Journal of Applied Biomedicine $x MED00012667
GRA    __
$a NT14330 $p MZ0
LZP    __
$c NLK188 $d 20170109 $a NLK 2016-34/dk

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace