• Je něco špatně v tomto záznamu ?

Detection of diketopiperazine and pyrrolnitrin, compounds with anti-Pythium insidiosum activity, in a Pseudomonas stutzeri environmental strain

Y. Thongsri, C. Aromdee, C. Yenjai, S. Kanokmedhakul, A. Chaiprasert, P. Hamal, C. Prariyachatigul

. 2014 ; 158 (3) : 378-383. [pub] 20121213

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16031507

AIMS: Screening of bacterial flora for strains producing metabolites with inhibitory effects on the human pathogenic oomycete Pythium insidiosum. Separation and characterization of extracts from Pseudomonas stutzeri with anti-Pythium inhibitory activity. Search for genes with anti-Pythium effect within the genome of P. stutzeri. METHODS: A total of 88 bacterial strains were isolated from water resources in northeastern Thailand. Two screening methods were used to establish their inhibitory effects on P. insidiosum. One strain, P. stutzeri ST1302 was randomly chosen, and the extract with anti-P. insidiosum activity was fractionated and subfractionated using liquid column chromatography and purified by thin layer chromatography. The chemical structure of purified fractions was determined by Fourier transform infrared spectroscopy, nuclear magnetic resonance and mass spectrometry. Further, search for genes involved in the anti-Pythium activity (phenazine-1-carboxylic acid, 2,4-diacetylphloroglucinol, pyoluteorin and pyrrolnitrin) was undertaken in this P. stutzeri strain using primers described in the literature. RESULTS: Anti-P. insidiosum activity was detected in 16 isolates (18.2%). In P. stutzeri ST1302, a subfraction labeled PYK7 exhibited strong activity against this oomycete. It was assigned to the diketopiperazines as cyclo(D-Pro-L-Val). In the search for genes, one gene region was successfully amplified. This corresponded to pyrrolnitrin. The results suggest the possibility of using the related metabolites against P. insidiosum. This is the first report on the inhibitory effects of P. stutzeri against this oomycete. The results may contribute to the development of antimicrobial drugs/probiotics against pythiosis.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16031507
003      
CZ-PrNML
005      
20161122095904.0
007      
ta
008      
161103s2014 xr d f 000 0|ENG||
009      
AR
024    7_
$a 10.5507/bp.2012.090 $2 doi
035    __
$a (PubMed)23149469
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a ENG
044    __
$a xr
100    1_
$a Thongsri, Yordhathai $u Faculty of Graduated School, Khon Kaen University, Khon Kaen, Thailand
245    10
$a Detection of diketopiperazine and pyrrolnitrin, compounds with anti-Pythium insidiosum activity, in a Pseudomonas stutzeri environmental strain / $c Y. Thongsri, C. Aromdee, C. Yenjai, S. Kanokmedhakul, A. Chaiprasert, P. Hamal, C. Prariyachatigul
520    9_
$a AIMS: Screening of bacterial flora for strains producing metabolites with inhibitory effects on the human pathogenic oomycete Pythium insidiosum. Separation and characterization of extracts from Pseudomonas stutzeri with anti-Pythium inhibitory activity. Search for genes with anti-Pythium effect within the genome of P. stutzeri. METHODS: A total of 88 bacterial strains were isolated from water resources in northeastern Thailand. Two screening methods were used to establish their inhibitory effects on P. insidiosum. One strain, P. stutzeri ST1302 was randomly chosen, and the extract with anti-P. insidiosum activity was fractionated and subfractionated using liquid column chromatography and purified by thin layer chromatography. The chemical structure of purified fractions was determined by Fourier transform infrared spectroscopy, nuclear magnetic resonance and mass spectrometry. Further, search for genes involved in the anti-Pythium activity (phenazine-1-carboxylic acid, 2,4-diacetylphloroglucinol, pyoluteorin and pyrrolnitrin) was undertaken in this P. stutzeri strain using primers described in the literature. RESULTS: Anti-P. insidiosum activity was detected in 16 isolates (18.2%). In P. stutzeri ST1302, a subfraction labeled PYK7 exhibited strong activity against this oomycete. It was assigned to the diketopiperazines as cyclo(D-Pro-L-Val). In the search for genes, one gene region was successfully amplified. This corresponded to pyrrolnitrin. The results suggest the possibility of using the related metabolites against P. insidiosum. This is the first report on the inhibitory effects of P. stutzeri against this oomycete. The results may contribute to the development of antimicrobial drugs/probiotics against pythiosis.
650    _2
$a diketopiperaziny $x farmakologie $7 D054659
650    _2
$a genom bakteriální $7 D016680
650    _2
$a mikrobiální testy citlivosti $7 D008826
650    _2
$a Pseudomonas stutzeri $x chemie $x genetika $x izolace a purifikace $7 D044223
650    _2
$a pyrrolnitrin $x farmakologie $7 D011764
650    _2
$a pythióza $x farmakoterapie $x mikrobiologie $7 D058968
650    _2
$a Pythium $x účinky léků $7 D011775
651    _2
$a Thajsko $7 D013785
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Aromdee, Chantana $u Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand $7 gn_A_00008779
700    1_
$a Yenjai, Chavi $u Department of Organic Chemistry, Faculty of Sciences, Khon Kaen University, Khon Kaen, Thailand
700    1_
$a Kanokmedhakul, Somdej $u Department of Organic Chemistry, Faculty of Sciences, Khon Kaen University, Khon Kaen, Thailand
700    1_
$a Chaiprasert, Angkana $u Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
700    1_
$a Hamal, Petr $u Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Czech Republic
700    1_
$a Prariyachatigul, Chularut $u Centre for Research and Development of Medical Diagnosis Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand
773    0_
$w MED00012606 $t Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czech Republic $x 1213-8118 $g Roč. 158, č. 3 (2014), s. 378-383
856    41
$u http://biomed.papers.upol.cz/ $y domovská stránka časopisu
910    __
$a ABA008 $b A 1502 $c 958 $y 4 $z 0
990    __
$a 20161103 $b ABA008
991    __
$a 20161116084902 $b ABA008
999    __
$a ok $b bmc $g 1173640 $s 956173
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 158 $c 3 $d 378-383 $e 20121213 $i 1213-8118 $m Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic $n Biomed. Pap. Fac. Med. Palacký Univ. Olomouc Czech Repub. (Print) $x MED00012606
LZP    __
$b NLK118 $a Pubmed-20161103

Najít záznam

Citační ukazatele

    Možnosti archivace