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The isolation and characterization of actinobacteria from dominant benthic macroinvertebrates endemic to Lake Baikal
D. Axenov-Gribanov, Y. Rebets, B. Tokovenko, I. Voytsekhovskaya, M. Timofeyev, A. Luzhetskyy,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Actinobacteria chemistry classification isolation & purification metabolism MeSH
- Amphipoda microbiology MeSH
- Anti-Infective Agents chemistry metabolism MeSH
- Bacteria drug effects MeSH
- Invertebrates microbiology MeSH
- Phylogeny MeSH
- Insecta microbiology MeSH
- Fungi drug effects MeSH
- Lakes microbiology MeSH
- Porifera microbiology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The high demand for new antibacterials fosters the isolation of new biologically active compounds producing actinobacteria. Here, we report the isolation and initial characterization of cultured actinobacteria from dominant benthic organisms' communities of Lake Baikal. Twenty-five distinct strains were obtained from 5 species of Baikal endemic macroinvertebrates of amphipods, freshwater sponges, turbellaria worms, and insects (caddisfly larvae). The 16S ribosomal RNA (rRNA)-based phylogenic analysis of obtained strains showed their affiliation to Streptomyces, Nocardia, Pseudonocardia, Micromonospora, Aeromicrobium, and Agromyces genera, revealing the diversity of actinobacteria associated with the benthic organisms of Lake Baikal. The biological activity assays showed that 24 out of 25 strains are producing compounds active against at least one of the test cultures used, including Gram-negative bacteria and Candida albicans. Complete dereplication of secondary metabolite profiles of two isolated strains led to identification of only few known compounds, while the majority of detected metabolites are not listed in existing antibiotic databases.
Helmholtz Institute for Pharmaceutical Research Saarland Saarbrucken Germany
Institute of Biology at Irkutsk State University Irkutsk Russia
References provided by Crossref.org
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