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NAS agar is more suitable than McKay agar for primary culture of Streptococcus milleri group (SMG) fastidious bacteria, S. intermedius in particular
V. Raclavsky, R. Novotny, L. Stary, L. Navratilova, J. Zatloukal, P. Jakubec, M. Zapalka, F. Kopriva, V. Kolek,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu srovnávací studie, hodnotící studie, časopisecké články
Grantová podpora
NT13560
MZ0
CEP - Centrální evidence projektů
- MeSH
- agar MeSH
- bakteriologické techniky metody MeSH
- kultivační média chemie MeSH
- lidé MeSH
- skupina Streptococcus milleri izolace a purifikace MeSH
- streptokokové infekce diagnóza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- srovnávací studie MeSH
Streptococcus milleri group (SMG) is a group of three streptococcal species (S. anginosus, intermedius and constellatus) that act as opportunist pathogens, among others in cystic fibrosis. Due to their fastidious character, they are both difficult to cultivate and to differentiate from less pathogenic streptococcal species, therefore being most probably underdiagnosed. Semi-selective McKay agar and NAS agar were developed to facilitate SMG recovery from clinical samples; however, direct comparison of recovery rates has not been published yet. We tested the performance of both media on 123 patient samples and demonstrated general superiority of NAS agar for SMG recovery during primary cultivation convincingly. This observation was also confirmed by quantitative drop tests during subculture. Despite the undisputed overall superiority of NAS agar over McKay agar, a smaller fraction of strains grew better on McKay agar. Inter-strain differences were the most probable explanation. Therefore, when economic conditions are not limiting and maximum recovery rate is desirable, both plates are advised to be used in parallel for primary cultivation of clinical samples.
Department of Pediatrics University Hospital Olomouc 1 P Pavlova 6 Olomouc Czech Republic
Department of Respiratory Medicine University Hospital Olomouc 1 P Pavlova 6 Olomouc Czech Republic
Department of Surgery 1 University Hospital Olomouc 1 P Pavlova 6 Olomouc Czech Republic
Citace poskytuje Crossref.org
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- $a Raclavský, Vladislav $u Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Hnevotinska 3, Olomouc, Czech Republic. vladislav.raclavsky@upol.cz. Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University Olomouc, Hnevotinska 5, Olomouc, Czech Republic. vladislav.raclavsky@upol.cz. $7 xx0046827
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- $a NAS agar is more suitable than McKay agar for primary culture of Streptococcus milleri group (SMG) fastidious bacteria, S. intermedius in particular / $c V. Raclavsky, R. Novotny, L. Stary, L. Navratilova, J. Zatloukal, P. Jakubec, M. Zapalka, F. Kopriva, V. Kolek,
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- $a Streptococcus milleri group (SMG) is a group of three streptococcal species (S. anginosus, intermedius and constellatus) that act as opportunist pathogens, among others in cystic fibrosis. Due to their fastidious character, they are both difficult to cultivate and to differentiate from less pathogenic streptococcal species, therefore being most probably underdiagnosed. Semi-selective McKay agar and NAS agar were developed to facilitate SMG recovery from clinical samples; however, direct comparison of recovery rates has not been published yet. We tested the performance of both media on 123 patient samples and demonstrated general superiority of NAS agar for SMG recovery during primary cultivation convincingly. This observation was also confirmed by quantitative drop tests during subculture. Despite the undisputed overall superiority of NAS agar over McKay agar, a smaller fraction of strains grew better on McKay agar. Inter-strain differences were the most probable explanation. Therefore, when economic conditions are not limiting and maximum recovery rate is desirable, both plates are advised to be used in parallel for primary cultivation of clinical samples.
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