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Stanovení glutathionu a glutathiondisulfidu v biologických vzorcích
[Determination of glutathione and glutathione disulfide in biological samples]
Roman Kanďár
Language Czech Country Czech Republic
- Keywords
- technika suché kapky krve,
- MeSH
- Glutathione analysis physiology metabolism MeSH
- Glutathione Disulfide analysis physiology metabolism MeSH
- Clinical Laboratory Techniques MeSH
- Humans MeSH
- Blood Specimen Collection MeSH
- Oxidative Stress MeSH
- Check Tag
- Humans MeSH
In the present review, methods for the determination of glutathione and glutathione disulfide are discussed. The main topic of this review is the suitable sample preparation prior to analysis which is absolutely crucial. The role of glutathione as a marker of oxidative stress is small; however, the ability of glutathione to adequately respond to oxidative stress by increasing the content of its oxidized form, especially glutathione disulfide, is meaningful. Therefore, methods that can properly quantify both forms of glutathione are essential to monitor the oxidative stress in humans.
Determination of glutathione and glutathione disulfide in biological samples
Literatura
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- $a In the present review, methods for the determination of glutathione and glutathione disulfide are discussed. The main topic of this review is the suitable sample preparation prior to analysis which is absolutely crucial. The role of glutathione as a marker of oxidative stress is small; however, the ability of glutathione to adequately respond to oxidative stress by increasing the content of its oxidized form, especially glutathione disulfide, is meaningful. Therefore, methods that can properly quantify both forms of glutathione are essential to monitor the oxidative stress in humans.
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