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Stanovení glutathionu a glutathiondisulfidu v biologických vzorcích
[Determination of glutathione and glutathione disulfide in biological samples]
Roman Kanďár
Jazyk čeština Země Česko
- Klíčová slova
- technika suché kapky krve,
- MeSH
- glutathion analýza fyziologie metabolismus MeSH
- glutathiondisulfid analýza fyziologie metabolismus MeSH
- klinické laboratorní techniky MeSH
- lidé MeSH
- odběr vzorku krve MeSH
- oxidační stres MeSH
- Check Tag
- lidé MeSH
In the present review, methods for the determination of glutathione and glutathione disulfide are discussed. The main topic of this review is the suitable sample preparation prior to analysis which is absolutely crucial. The role of glutathione as a marker of oxidative stress is small; however, the ability of glutathione to adequately respond to oxidative stress by increasing the content of its oxidized form, especially glutathione disulfide, is meaningful. Therefore, methods that can properly quantify both forms of glutathione are essential to monitor the oxidative stress in humans.
Determination of glutathione and glutathione disulfide in biological samples
Literatura
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- $a In the present review, methods for the determination of glutathione and glutathione disulfide are discussed. The main topic of this review is the suitable sample preparation prior to analysis which is absolutely crucial. The role of glutathione as a marker of oxidative stress is small; however, the ability of glutathione to adequately respond to oxidative stress by increasing the content of its oxidized form, especially glutathione disulfide, is meaningful. Therefore, methods that can properly quantify both forms of glutathione are essential to monitor the oxidative stress in humans.
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