Certain non-steroidal anti-inflammatory drugs can inhibit fungal growth, fungal prostaglandin E2 production, and enzyme activation. This study aims to investigate the antifungal effect of nimesulide against pathogenic filamentous fungi and yeast. The experiments detailed below were also designed to investigate whether the action is dependent on E2 fungal prostaglandins. Our data showed that nimesulide exhibited potent antifungal activity, mainly against Trichophyton mentagrophytes (ATCC 9533) and Cryptococcus neoformans with MIC values of 2 and 62 μg/mL, respectively. This drug was also able to inhibit the growth of clinic isolates of filamentous fungi, such as Aspergillus fumigatus, and dermatophytes, such as T. rubrum, T. mentagrophytes, Epidermophyton floccosum, Microsporum canis, and M. gypseum, with MIC values ranging from 112 to 770 μg/mL. Our data also showed that the inhibition of fungal growth by nimesulide was mediated by a mechanism dependent on PGE2, which led to the inhibition of essential fungal enzymes. Thus, we concluded that nimesulide exerts a fungicidal effect against pathogenic filamentous fungi and yeast, involving the inhibition of fungal prostaglandins and fungal enzymes important to the fungal growth and colonization.

Programa de Pós graduação em Ciências Farmacêuticas Faculdade de Farmácia Universidade Federal do Pará UFPA Rua Augusto Corrêa 01 Bairro Guamá Belém PA 66075 110 Brazil

Programa de Pós graduação em Ciências Farmacêuticas Programa de Pós graduação Neurociências e Biologia Celular Programa de Pós graduação em Química Medicinal e Modelagem Molecular Instituto de Ciências da Saúde Universidade Federal do Pará UFPA Belém PA 66075 110 Brazil

000      

00000naa a2200000 a 4500

001      

bmc17010569

003      

CZ-PrNML

005      

20170321103331.0

007      

ta

008      

170321s2017 xxu f 000 0|eng||

009      

AR

024    7_

$a 10.1007/s12223-016-0483-6 $2 doi

035    __

$a (PubMed)27866353

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxu

100    1_

$a de Matos, Rafaelle Fonseca $u Programa de Pós-graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Pará/UFPA, Rua Augusto Corrêa, 01, Bairro Guamá, Belém, PA, 66075-110, Brazil.

245    10

$a Nimesulide inhibits pathogenic fungi: PGE2-dependent mechanisms / $c RF. de Matos, LC. Mendonça, KG. da Silva Souza, AA. Fonseca, EM. Costa, MV. de Lima, JM. Vieira, MT. de Brito, MC. Monteiro,

520    9_

$a Certain non-steroidal anti-inflammatory drugs can inhibit fungal growth, fungal prostaglandin E2 production, and enzyme activation. This study aims to investigate the antifungal effect of nimesulide against pathogenic filamentous fungi and yeast. The experiments detailed below were also designed to investigate whether the action is dependent on E2 fungal prostaglandins. Our data showed that nimesulide exhibited potent antifungal activity, mainly against Trichophyton mentagrophytes (ATCC 9533) and Cryptococcus neoformans with MIC values of 2 and 62 μg/mL, respectively. This drug was also able to inhibit the growth of clinic isolates of filamentous fungi, such as Aspergillus fumigatus, and dermatophytes, such as T. rubrum, T. mentagrophytes, Epidermophyton floccosum, Microsporum canis, and M. gypseum, with MIC values ranging from 112 to 770 μg/mL. Our data also showed that the inhibition of fungal growth by nimesulide was mediated by a mechanism dependent on PGE2, which led to the inhibition of essential fungal enzymes. Thus, we concluded that nimesulide exerts a fungicidal effect against pathogenic filamentous fungi and yeast, involving the inhibition of fungal prostaglandins and fungal enzymes important to the fungal growth and colonization.

650    _2

$a antifungální látky $x farmakologie $7 D000935

650    _2

$a Arthrodermataceae $x účinky léků $x růst a vývoj $x metabolismus $7 D003883

650    _2

$a Aspergillus fumigatus $x účinky léků $x růst a vývoj $x metabolismus $7 D001232

650    _2

$a Cryptococcus neoformans $x účinky léků $x růst a vývoj $x metabolismus $7 D003455

650    _2

$a dinoproston $x antagonisté a inhibitory $x biosyntéza $7 D015232

650    _2

$a mikrobiální testy citlivosti $7 D008826

650    _2

$a sulfonamidy $x farmakologie $7 D013449

650    _2

$a Trichophyton $x účinky léků $x růst a vývoj $x metabolismus $7 D014249

655    _2

$a časopisecké články $7 D016428

700    1_

$a Mendonça, Lúcia Carla Vasconcelos $u Programa de Pós-graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Pará/UFPA, Rua Augusto Corrêa, 01, Bairro Guamá, Belém, PA, 66075-110, Brazil.

700    1_

$a da Silva Souza, Kaira Geiliane $u Programa de Pós-graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Pará/UFPA, Rua Augusto Corrêa, 01, Bairro Guamá, Belém, PA, 66075-110, Brazil.

700    1_

$a Fonseca, Adriana Aparecida Durães $u Programa de Pós-graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Pará/UFPA, Rua Augusto Corrêa, 01, Bairro Guamá, Belém, PA, 66075-110, Brazil.

700    1_

$a Costa, Elaine Mirla Souza $u Programa de Pós-graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Pará/UFPA, Rua Augusto Corrêa, 01, Bairro Guamá, Belém, PA, 66075-110, Brazil.

700    1_

$a de Lima, Marcus Vinicius Dias $u Programa de Pós-graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Pará/UFPA, Rua Augusto Corrêa, 01, Bairro Guamá, Belém, PA, 66075-110, Brazil.

700    1_

$a Vieira, José Maria Dos Santos $u Programa de Pós-graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Pará/UFPA, Rua Augusto Corrêa, 01, Bairro Guamá, Belém, PA, 66075-110, Brazil.

700    1_

$a de Brito, Mioni Thielli Figueiredo Magalhães $u Programa de Pós-graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Pará/UFPA, Rua Augusto Corrêa, 01, Bairro Guamá, Belém, PA, 66075-110, Brazil.

700    1_

$a Monteiro, Marta Chagas $u Programa de Pós-graduação em Ciências Farmacêuticas, Programa de Pós-graduação Neurociências e Biologia Celular, Programa de Pós-graduação em Química Medicinal e Modelagem Molecular, Instituto de Ciências da Saúde, Universidade Federal do Pará/UFPA, Belém, PA, 66075-110, Brazil. martachagas2@yahoo.com.br.

773    0_

$w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 62, č. 2 (2017), s. 169-174

856    41

$u https://pubmed.ncbi.nlm.nih.gov/27866353 $y Pubmed

910    __

$a ABA008 $b online $c sign $y a $z 0

990    __

$a 20170321 $b ABA008

991    __

$a 20170321103602 $b ABA008

999    __

$a ok $b bmc $g 1195192 $s 971285

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2017 $b 62 $c 2 $d 169-174 $e 20161119 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005

LZP    __

$a Pubmed-20170321