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Factors affecting interactions between sulphonate-terminated dendrimers and proteins: A three case study
E. González-García, M. Maly, FJ. de la Mata, R. Gómez, ML. Marina, MC. García,
Language English Country Netherlands
Document type Journal Article
- MeSH
- Dendrimers chemistry MeSH
- Spectrometry, Fluorescence MeSH
- Isoelectric Point MeSH
- Hydrogen-Ion Concentration MeSH
- Horses MeSH
- Chickens MeSH
- Molecular Weight MeSH
- Muramidase chemistry MeSH
- Myoglobin chemistry MeSH
- Serum Albumin, Bovine chemistry MeSH
- Silanes chemistry MeSH
- Molecular Dynamics Simulation MeSH
- Cattle MeSH
- Static Electricity MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
This work proposes a deep study on the interactions between sulphonate-terminated carbosilane dendrimers and proteins. Three different proteins with different molecular weights and isoelectric points were employed and different pHs, dendrimer concentrations and generations were tested. Variations in fluorescence intensity and emission wavelength were used as protein-dendrimer interaction probes. Interaction between dendrimers and proteins greatly depended on the protein itself and pH. Other important issues were the dendrimer concentration and generation. Protein-dendrimer interactions were favored under acidic working conditions when proteins were positively charged. Moreover, in general, high dendrimer generations promoted these interactions. Modeling of protein-dendrimer interactions allowed to understand the different behaviors observed for every protein.
References provided by Crossref.org
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- $a González-García, Estefanía $u Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain.
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- $a Factors affecting interactions between sulphonate-terminated dendrimers and proteins: A three case study / $c E. González-García, M. Maly, FJ. de la Mata, R. Gómez, ML. Marina, MC. García,
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- $a This work proposes a deep study on the interactions between sulphonate-terminated carbosilane dendrimers and proteins. Three different proteins with different molecular weights and isoelectric points were employed and different pHs, dendrimer concentrations and generations were tested. Variations in fluorescence intensity and emission wavelength were used as protein-dendrimer interaction probes. Interaction between dendrimers and proteins greatly depended on the protein itself and pH. Other important issues were the dendrimer concentration and generation. Protein-dendrimer interactions were favored under acidic working conditions when proteins were positively charged. Moreover, in general, high dendrimer generations promoted these interactions. Modeling of protein-dendrimer interactions allowed to understand the different behaviors observed for every protein.
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- $a de la Mata, Francisco Javier $u Departamento de Química Orgánica y Química Inorgánica, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain. CIBER-BBN, Spain.
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- $a Gómez, Rafael $u Departamento de Química Orgánica y Química Inorgánica, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain. CIBER-BBN, Spain.
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- $a Marina, María Luisa $u Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain.
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- $a García, María Concepción $u Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain. Electronic address: concepcion.garcia@uah.es.
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