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Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells

K. Vocetkova, M. Buzgo, V. Sovkova, D. Bezdekova, P. Kneppo, E. Amler,

. 2016 ; 49 (5) : 568-78. [pub] 20160724

Language English Country England, Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc17013638

Grant support
NV15-33094A MZ0 CEP Register

OBJECTIVES: Faulty wound healing is a global healthcare problem. Chronic wounds are generally characterized by a reduction in availability of growth factors. New strategies are being developed to deliver growth factors more effectively. METHODS: In this study, we introduced electrospun scaffolds composed of polycaprolactone (PCL) nanofibers functionalized with adhered platelets, as a source of numerous growth factors. Three concentrations of platelets were immobilized to nanofibrous scaffolds by simple adhesion, and their influence on adhesion, proliferation and metabolic activity of seeded cells (murine fibroblasts, keratinocytes and melanocytes) was investigated. RESULTS: The data obtained indicated that presence of platelets significantly promoted cell spreading, proliferation and metabolic activity in all the skin-associated cell types. There were no significant differences among tested concentrations of platelets, thus even the lowest concentration sufficiently promoted proliferation of the seeded cells. CONCLUSIONS: Such complex stimulation is needed for improved healing of chronic wounds. However, the nanofibrous system can be used not only as a skin cover, but also in broader applications in regenerative medicine.

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$a Vocetkova, K $u Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, 150 06, Prague 5, Czech Republic. karolina_vocetkova@labdemo.cz. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, 142 20, Prague 4, Czech Republic. karolina_vocetkova@labdemo.cz. University Center for Energy Efficient Buildings, Czech Technical University in Prague, 273 43, Bustehrad, Czech Republic. karolina_vocetkova@labdemo.cz. Faculty of Biomedical Engineering, Czech Technical University in Prague, 272 01, Kladno 2, Czech Republic. karolina_vocetkova@labdemo.cz.
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$a Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells / $c K. Vocetkova, M. Buzgo, V. Sovkova, D. Bezdekova, P. Kneppo, E. Amler,
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$a OBJECTIVES: Faulty wound healing is a global healthcare problem. Chronic wounds are generally characterized by a reduction in availability of growth factors. New strategies are being developed to deliver growth factors more effectively. METHODS: In this study, we introduced electrospun scaffolds composed of polycaprolactone (PCL) nanofibers functionalized with adhered platelets, as a source of numerous growth factors. Three concentrations of platelets were immobilized to nanofibrous scaffolds by simple adhesion, and their influence on adhesion, proliferation and metabolic activity of seeded cells (murine fibroblasts, keratinocytes and melanocytes) was investigated. RESULTS: The data obtained indicated that presence of platelets significantly promoted cell spreading, proliferation and metabolic activity in all the skin-associated cell types. There were no significant differences among tested concentrations of platelets, thus even the lowest concentration sufficiently promoted proliferation of the seeded cells. CONCLUSIONS: Such complex stimulation is needed for improved healing of chronic wounds. However, the nanofibrous system can be used not only as a skin cover, but also in broader applications in regenerative medicine.
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$a Buzgo, M $u Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, 150 06, Prague 5, Czech Republic. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, 142 20, Prague 4, Czech Republic. University Center for Energy Efficient Buildings, Czech Technical University in Prague, 273 43, Bustehrad, Czech Republic.
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