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Use of In Vitro Transcription System for Analysis of Corynebacterium glutamicum Promoters Recognized by Two Sigma Factors
R. Šilar, J. Holátko, L. Rucká, A. Rapoport, H. Dostálová, P. Kadeřábková, J. Nešvera, M. Pátek,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
NLK
ProQuest Central
od 1997-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2000-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 1997-01-01 do Před 1 rokem
- MeSH
- bakteriální proteiny genetika metabolismus MeSH
- Corynebacterium glutamicum genetika metabolismus MeSH
- genetická transkripce * MeSH
- promotorové oblasti (genetika) * MeSH
- regulace genové exprese u bakterií * MeSH
- sigma faktor genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
Promoter activities in Corynebacterium glutamicum strains with deletions of genes encoding sigma factors of RNA polymerase suggested that transcription from some promoters is controlled by two sigma factors. To prove that different sigma factors are involved in the recognition of selected Corynebacterium glutamicum promoters, in vitro transcription system was applied. It was found that a typical housekeeping promoter Pper interacts with the alternative sigma factor σ(B) in addition to the primary sigma factor σ(A). On the other way round, the σ(B)-dependent promoter of the pqo gene that is expressed mainly in the stationary growth phase was active also with σ(A). Some promoters of genes involved in stress responses (P1clgR, P2dnaK, and P2dnaJ2) were found to be recognized by two stress-responding sigma factors, σ(H) and σ(E). In vitro transcription system thus proved to be a useful direct technique for demonstrating the overlap of different sigma factors in recognition of individual promoters in C. glutamicum.
Institute of Microbiology Czech Academy of Sciences v v i Vídeňská 1083 142 20 Prague Czech Republic
Citace poskytuje Crossref.org
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