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Microsatellite markers for Cryptolestes ferrugineus (Coleoptera: Laemophloeidae) and other Cryptolestes species
Y. Wu, F. Li, Z. Li, V. Stejskal, Z. Kučerová, G. Opit, R. Aulicky, T. Zhang, P. He, Y. Cao,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
ProQuest Central
od 2001-02-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2001-02-01 do Před 1 rokem
- MeSH
- brouci klasifikace genetika fyziologie MeSH
- jedlá semena parazitologie MeSH
- mikrosatelitní repetice * MeSH
- polymorfismus genetický genetika MeSH
- techniky amplifikace nukleových kyselin MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Cryptolestes ferrugineus (Stephens, 1831) is an important insect pest of stored products. Due to its broad host range, short life cycle, and high reproductive capacity, this species has rapidly colonized temperate and tropical regions around the world. In this study, we isolated 18 novel polymorphic microsatellite loci from an enriched genomic library based on a biotin/streptavidin capture protocol. These loci will be useful tool to better understand the genetic structure and migration patterns of C. ferrugineus throughout the world. The genetic parameters were estimated based on 80 individual C. ferrugineus from two natural populations. The results revealed that 18 loci were different polymorphic levels. The numbers of alleles ranged from 3 to 12, and eleven loci demonstrated polymorphic information contents greater than 0.5. The observed (H O) and expected (H E) heterozygosities ranged from 0.051 to 0.883 and 0.173 to 0.815, respectively. Five locus/population combinations significantly deviated from Hardy-Weinberg equilibrium. We also demonstrated the potential utility of the C. ferrugineus microsatellites as population and species markers for four additional Cryptolestes species.
Academy of State Administration of Grain No 11 Baiwanzhuang Street Beijing China
Department of Entomology China Agricultural University No 2 Yuanmingyuan West Road Beijing China
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