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Histological evaluation of decellularized skeletal muscle tissue using two different decellularization agents
H. Hrebíková, M. Voborníková, M. Hetešová, J. Mokrý
Language English Country Czech Republic
Document type Journal Article
Digital library NLK
Full text - Article
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NLK
Directory of Open Access Journals
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- MeSH
- Coloring Agents MeSH
- Sodium Dodecyl Sulfate pharmacology MeSH
- Elastic Tissue diagnostic imaging drug effects MeSH
- Glycosaminoglycans MeSH
- Collagen drug effects MeSH
- Muscle, Skeletal cytology drug effects MeSH
- Microscopy MeSH
- Mice MeSH
- Octoxynol pharmacology MeSH
- Surface-Active Agents pharmacology MeSH
- Tissue Scaffolds * MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
The aim of the present study was to determine effect of two decellularized agents, sodium dodecyl sulphate (SDS) and Triton X-100, to the skeletal muscle tissue. Final scaffold was evaluated by several histological techniques to analyse preservation of essential structures including collagen and elastic fibres, basement membranes, glycosaminoglycans and also to confirm elimination of nuclear and cytoplasmic components which are redundant in effectively prepared decellularized scaffolds. Comparison of tissue scaffolds processed with different detergents proved that SDS is superior to Triton X-100 as it can effectively decellularize muscle tissue.
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