• Something wrong with this record ?

Altered thyroid status affects myocardial expression of connexin-43 and susceptibility of rat heart to malignant arrhythmias that can be partially normalized by red palm oil intake

BS. Bačová, C. Vinczenzová, J. Žurmanová, D. Kašparová, V. Knezl, TE. Beňová, S. Pavelka, T. Soukup, N. Tribulová,

. 2017 ; 147 (1) : 63-73. [pub] 20160906

Language English Country Germany

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc17023722
E-resources Online Full text

NLK ProQuest Central from 1997-01-01 to 1 year ago
Medline Complete (EBSCOhost) from 2000-01-01 to 1 year ago
Nursing & Allied Health Database (ProQuest) from 1997-01-01 to 1 year ago
Health & Medicine (ProQuest) from 1997-01-01 to 1 year ago
Public Health Database (ProQuest) from 1997-01-01 to 1 year ago

Links

PubMed 27600718
DOI 10.1007/s00418-016-1488-6
Knihovny.cz E-resources

We aimed to study the impact of altered thyroid status on myocardial expression of electrical coupling protein connexin-43 (Cx43), the susceptibility of rats to ventricular fibrillation (VF) and the effects of antioxidant-rich red palm oil (RPO). Adult male and female euthyroid, hyperthyroid (treated with T3/T4), hypothyroid (treated with methimazole) Wistar rats supplemented or not with RPO for 6 weeks were used. Function of isolated perfused heart and VF threshold were determined. Left ventricular tissue was used for assessment of mRNA and protein levels of Cx43, its phosphorylated forms and topology. Protein kinase C signaling (PKC) and gene transcripts of some proteins related to cardiac arrhythmias were assessed. Hyperthyroid state resulted in decrease of total and phosphorylated forms of Cx43 and suppression of PKC-ε expression in males and females, decrease of Cx43 mRNA in females, decrease of VF threshold and increase of functional parameters in male rat hearts. In contrast, hypothyroid status resulted in the increase of total and phosphorylated forms of Cx43, enhancement PKC-ε expression in males and females, increase of Cx43 mRNA in females, increase of VF threshold and decrease of functional parameters in male rat hearts. Function of the heart was partially normalized by RPO intake, which also enhanced myocardial Cx43 and PKC-ε expression as well as increased VF threshold in hyperthyroid male rats. We conclude that there is an inverse relationship between myocardial expression of Cx43, including its functional phosphorylated forms, and susceptibility of male rat hearts to VF in condition of altered thyroid status. RPO intake partly ameliorated adverse changes caused by excess of thyroid hormones.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17023722
003      
CZ-PrNML
005      
20181119073018.0
007      
ta
008      
170720s2017 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s00418-016-1488-6 $2 doi
035    __
$a (PubMed)27600718
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Bačová, Barbara Szeiffová $u Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, POBox 104, 840 05, Bratislava, Slovak Republic.
245    10
$a Altered thyroid status affects myocardial expression of connexin-43 and susceptibility of rat heart to malignant arrhythmias that can be partially normalized by red palm oil intake / $c BS. Bačová, C. Vinczenzová, J. Žurmanová, D. Kašparová, V. Knezl, TE. Beňová, S. Pavelka, T. Soukup, N. Tribulová,
520    9_
$a We aimed to study the impact of altered thyroid status on myocardial expression of electrical coupling protein connexin-43 (Cx43), the susceptibility of rats to ventricular fibrillation (VF) and the effects of antioxidant-rich red palm oil (RPO). Adult male and female euthyroid, hyperthyroid (treated with T3/T4), hypothyroid (treated with methimazole) Wistar rats supplemented or not with RPO for 6 weeks were used. Function of isolated perfused heart and VF threshold were determined. Left ventricular tissue was used for assessment of mRNA and protein levels of Cx43, its phosphorylated forms and topology. Protein kinase C signaling (PKC) and gene transcripts of some proteins related to cardiac arrhythmias were assessed. Hyperthyroid state resulted in decrease of total and phosphorylated forms of Cx43 and suppression of PKC-ε expression in males and females, decrease of Cx43 mRNA in females, decrease of VF threshold and increase of functional parameters in male rat hearts. In contrast, hypothyroid status resulted in the increase of total and phosphorylated forms of Cx43, enhancement PKC-ε expression in males and females, increase of Cx43 mRNA in females, increase of VF threshold and decrease of functional parameters in male rat hearts. Function of the heart was partially normalized by RPO intake, which also enhanced myocardial Cx43 and PKC-ε expression as well as increased VF threshold in hyperthyroid male rats. We conclude that there is an inverse relationship between myocardial expression of Cx43, including its functional phosphorylated forms, and susceptibility of male rat hearts to VF in condition of altered thyroid status. RPO intake partly ameliorated adverse changes caused by excess of thyroid hormones.
650    _2
$a aplikace orální $7 D000284
650    _2
$a zvířata $7 D000818
650    _2
$a srdeční arytmie $x farmakoterapie $x metabolismus $7 D001145
650    _2
$a konexin 43 $x antagonisté a inhibitory $x genetika $x metabolismus $7 D018031
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a srdce $x účinky léků $7 D006321
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a myokard $x metabolismus $7 D009206
650    _2
$a oleje rostlin $x aplikace a dávkování $x farmakologie $7 D010938
650    _2
$a messenger RNA $x antagonisté a inhibitory $x genetika $x metabolismus $7 D012333
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a potkani Wistar $7 D017208
650    _2
$a štítná žláza $x účinky léků $x metabolismus $7 D013961
655    _2
$a časopisecké články $7 D016428
700    1_
$a Vinczenzová, Csilla $u Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, POBox 104, 840 05, Bratislava, Slovak Republic.
700    1_
$a Žurmanová, Jitka $u Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic.
700    1_
$a Kašparová, Dita $u Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic. Institute of Physiology, v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Knezl, Vladimír $u Institute of Experimental Pharmacology and Toxicology, SAS, Bratislava, Slovak Republic.
700    1_
$a Egan Benová, Tamara, $d 1985- $7 xx0229479 $u Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, POBox 104, 840 05, Bratislava, Slovak Republic.
700    1_
$a Pavelka, Stanislav $u Institute of Physiology, v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Soukup, Tomáš $u Institute of Physiology, v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Tribulová, Narcisa $u Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, POBox 104, 840 05, Bratislava, Slovak Republic. narcisa.tribulova@savba.sk.
773    0_
$w MED00002042 $t Histochemistry and cell biology $x 1432-119X $g Roč. 147, č. 1 (2017), s. 63-73
856    41
$u https://pubmed.ncbi.nlm.nih.gov/27600718 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20170720 $b ABA008
991    __
$a 20181119073111 $b ABA008
999    __
$a ok $b bmc $g 1239403 $s 984635
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 147 $c 1 $d 63-73 $e 20160906 $i 1432-119X $m Histochemistry and cell biology $n Histochem Cell Biol $x MED00002042
LZP    __
$a Pubmed-20170720

Find record

Citation metrics

Archiving options