• Something wrong with this record ?

PLK1 regulates spindle formation kinetics and APC/C activation in mouse zygote

V. Baran, A. Brzakova, P. Rehak, V. Kovarikova, P. Solc,

. 2016 ; 24 (3) : 338-45. [pub] 20150715

Language English Country England, Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc17024240

Polo-like kinase 1 (PLK1) is involved in essential events of cell cycle including mitosis in which it participates in centrosomal microtubule nucleation, spindle bipolarity establishment and cytokinesis. Although PLK1 function has been studied in cycling cancer cells, only limited data are known about its role in the first mitosis of mammalian zygotes. During the 1-cell stage of mouse embryo development, the acentriolar spindle is formed and the shift from acentriolar to centrosomal spindle formation progresses gradually throughout the preimplantation stage, thus providing a unique possibility to study acentriolar spindle formation. We have shown previously that PLK1 activity is not essential for entry into first mitosis, but is required for correct spindle formation and anaphase onset in 1-cell mouse embryos. In the present study, we extend this knowledge by employing quantitative confocal live cell imaging to determine spindle formation kinetics in the absence of PLK1 activity and answer the question whether metaphase arrest at PLK1-inhibited embryos is associated with low anaphase-promoting complex/cyclosome (APC/C) activity and consequently high securin level. We have shown that inhibition of PLK1 activity induces a delay in onset of acentriolar spindle formation during first mitosis. Although these PLK1-inhibited 1-cell embryos were finally able to form a bipolar spindle, not all chromosomes were aligned at the metaphase equator. PLK1-inhibited embryos were arrested in metaphase without any sign of APC/C activation with high securin levels. Our results document that PLK1 controls the onset of spindle assembly and spindle formation, and is essential for APC/C activation before anaphase onset in mouse zygotes.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17024240
003      
CZ-PrNML
005      
20170830095625.0
007      
ta
008      
170720s2016 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1017/S0967199415000246 $2 doi
035    __
$a (PubMed)26174739
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Baran, Vladimir $u Institute of Animal Physiology,Slovak Academy of Sciences,Soltesovej 4,040 01 Kosice,Slovakia.
245    10
$a PLK1 regulates spindle formation kinetics and APC/C activation in mouse zygote / $c V. Baran, A. Brzakova, P. Rehak, V. Kovarikova, P. Solc,
520    9_
$a Polo-like kinase 1 (PLK1) is involved in essential events of cell cycle including mitosis in which it participates in centrosomal microtubule nucleation, spindle bipolarity establishment and cytokinesis. Although PLK1 function has been studied in cycling cancer cells, only limited data are known about its role in the first mitosis of mammalian zygotes. During the 1-cell stage of mouse embryo development, the acentriolar spindle is formed and the shift from acentriolar to centrosomal spindle formation progresses gradually throughout the preimplantation stage, thus providing a unique possibility to study acentriolar spindle formation. We have shown previously that PLK1 activity is not essential for entry into first mitosis, but is required for correct spindle formation and anaphase onset in 1-cell mouse embryos. In the present study, we extend this knowledge by employing quantitative confocal live cell imaging to determine spindle formation kinetics in the absence of PLK1 activity and answer the question whether metaphase arrest at PLK1-inhibited embryos is associated with low anaphase-promoting complex/cyclosome (APC/C) activity and consequently high securin level. We have shown that inhibition of PLK1 activity induces a delay in onset of acentriolar spindle formation during first mitosis. Although these PLK1-inhibited 1-cell embryos were finally able to form a bipolar spindle, not all chromosomes were aligned at the metaphase equator. PLK1-inhibited embryos were arrested in metaphase without any sign of APC/C activation with high securin levels. Our results document that PLK1 controls the onset of spindle assembly and spindle formation, and is essential for APC/C activation before anaphase onset in mouse zygotes.
650    _2
$a anafáze $7 D000705
650    _2
$a anafázi podporující komplex $x metabolismus $7 D064173
650    _2
$a zvířata $7 D000818
650    _2
$a blastocysta $7 D001755
650    _2
$a proteiny buněčného cyklu $x antagonisté a inhibitory $x metabolismus $7 D018797
650    _2
$a centrozom $x metabolismus $7 D018385
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a kinetika $7 D007700
650    _2
$a kinetochory $x metabolismus $7 D018386
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a myši $7 D051379
650    _2
$a konfokální mikroskopie $7 D018613
650    _2
$a mitóza $7 D008938
650    _2
$a protein-serin-threoninkinasy $x antagonisté a inhibitory $x metabolismus $7 D017346
650    _2
$a protoonkogenní proteiny $x antagonisté a inhibitory $x metabolismus $7 D011518
650    _2
$a pteridiny $x farmakologie $7 D011621
650    _2
$a aparát dělícího vřeténka $x metabolismus $7 D008941
650    _2
$a časosběrné zobrazování $7 D059008
650    _2
$a zygota $x účinky léků $x metabolismus $7 D015053
655    _2
$a časopisecké články $7 D016428
700    1_
$a Brzakova, Adela $u Institute of Animal Physiology and Genetics,Academy of Sciences of the Czech Republic,Libechov,Czech Republic.
700    1_
$a Rehak, Pavol $u Institute of Animal Physiology,Slovak Academy of Sciences,Kosice,Slovakia.
700    1_
$a Kovarikova, Veronika $u Institute of Animal Physiology,Slovak Academy of Sciences,Kosice,Slovakia.
700    1_
$a Solc, Petr $u Institute of Animal Physiology and Genetics,Academy of Sciences of the Czech Republic,Libechov,Czech Republic.
773    0_
$w MED00004864 $t Zygote (Cambridge, England) $x 1469-8730 $g Roč. 24, č. 3 (2016), s. 338-45
856    41
$u https://pubmed.ncbi.nlm.nih.gov/26174739 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20170720 $b ABA008
991    __
$a 20170830100213 $b ABA008
999    __
$a ok $b bmc $g 1239921 $s 985153
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2016 $b 24 $c 3 $d 338-45 $e 20150715 $i 1469-8730 $m Zygote $n Zygote $x MED00004864
LZP    __
$a Pubmed-20170720

Find record

Citation metrics

Archiving options