-
Je něco špatně v tomto záznamu ?
Species identification in forensic samples using the SPInDel approach: A GHEP-ISFG inter-laboratory collaborative exercise
C. Alves, R. Pereira, L. Prieto, M. Aler, CR. Amaral, C. Arévalo, G. Berardi, F. Di Rocco, M. Caputo, CH. Carmona, L. Catelli, HA. Costa, P. Coufalova, S. Furfuro, Ó. García, A. Gaviria, A. Goios, JJ. Gómez, A. Hernández, ED. Hernández, L....
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- druhová specificita * MeSH
- elektroforéza kapilární * MeSH
- kooperační chování MeSH
- laboratoře MeSH
- lidé MeSH
- multiplexová polymerázová řetězová reakce * MeSH
- RNA ribozomální genetika MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
DNA is a powerful tool available for forensic investigations requiring identification of species. However, it is necessary to develop and validate methods able to produce results in degraded and or low quality DNA samples with the high standards obligatory in forensic research. Here, we describe a voluntary collaborative exercise to test the recently developed Species Identification by Insertions/Deletions (SPInDel) method. The SPInDel kit allows the identification of species by the generation of numeric profiles combining the lengths of six mitochondrial ribosomal RNA (rRNA) gene regions amplified in a single reaction followed by capillary electrophoresis. The exercise was organized during 2014 by a Working Commission of the Spanish and Portuguese-Speaking Working Group of the International Society for Forensic Genetics (GHEP-ISFG), created in 2013. The 24 participating laboratories from 10 countries were asked to identify the species in 11 DNA samples from previous GHEP-ISFG proficiency tests using a SPInDel primer mix and control samples of the 10 target species. A computer software was also provided to the participants to assist the analyses of the results. All samples were correctly identified by 22 of the 24 laboratories, including samples with low amounts of DNA (hair shafts) and mixtures of saliva and blood. Correct species identifications were obtained in 238 of the 241 (98.8%) reported SPInDel profiles. Two laboratories were responsible for the three cases of misclassifications. The SPInDel was efficient in the identification of species in mixtures considering that only a single laboratory failed to detect a mixture in one sample. This result suggests that SPInDel is a valid method for mixture analyses without the need for DNA sequencing, with the advantage of identifying more than one species in a single reaction. The low frequency of wrong (5.0%) and missing (2.1%) alleles did not interfere with the correct species identification, which demonstrated the advantage of using a method based on the analysis of multiple loci. Overall, the SPInDel method was easily implemented by laboratories using different genotyping platforms, the interpretation of results was straightforward and the SPInDel software was used without any problems. The results of this collaborative exercise indicate that the SPInDel method can be applied successfully in forensic casework investigations.
Delegação do Norte Instituto Nacional de Medicina Legal e Ciências Forenses 1 P Porto Portugal
Faculty of Sciences University of Porto Porto Portugal
Institute of Criminalistics Prague Prague Czech Republic
Instituto de Investigação e Inovação em Saúde I3S Universidade do Porto Portugal
Instituto de Medicina Legal de Las Palmas Laboratorio Genética Forense Spain
Instituto de Patologia e Imunologia Molecular da Universidade do Porto Porto Portugal
Interdisciplinary Centre of Marine and Environmental Research University of Porto Porto Portugal
Laboratorio de Análisis de ADN Facultad de Ciencias Médicas UNCuyo Mendoza Argentina
Laboratório de Genética Aplicada Universidade de Aveiro Portugal
Laboratorio de Genética Molecular Cruz Roja Ecuatoriana Cruz Vital Quito Ecuador
Laboratorio del Equipo Argentino de Antropología Forense Córdoba Argentina
PRICAI Fundación Favaloro CABA Argentina
Sección de Bioquímica Departamento de Ciencias Forenses Poder Judicial de Costa Rica Costa Rica
Servicio de Criminalística de la Guardia Civil Departamento de Química y Medio Ambiente Madrid Spain
Unidad de Laboratorio de Navarra de Servicios y Tecnologías Villava Spain
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc17030946
- 003
- CZ-PrNML
- 005
- 20171102104802.0
- 007
- ta
- 008
- 171025s2017 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.fsigen.2017.03.003 $2 doi
- 035 __
- $a (PubMed)28324847
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Alves, Cíntia $u Instituto de Investigação e Inovação em Saúde, I3S, Universidade do Porto, Portugal; Instituto de Patologia e Imunologia Molecular da Universidade do Porto (IPATIMUP), Porto, Portugal. Electronic address: calves@ipatimup.pt. $7 gn_A_00005140
- 245 10
- $a Species identification in forensic samples using the SPInDel approach: A GHEP-ISFG inter-laboratory collaborative exercise / $c C. Alves, R. Pereira, L. Prieto, M. Aler, CR. Amaral, C. Arévalo, G. Berardi, F. Di Rocco, M. Caputo, CH. Carmona, L. Catelli, HA. Costa, P. Coufalova, S. Furfuro, Ó. García, A. Gaviria, A. Goios, JJ. Gómez, A. Hernández, ED. Hernández, L. Miranda, D. Parra, S. Pedrosa, MJ. Porto, ML. Rebelo, M. Spirito, MD. Torres, A. Amorim, F. Pereira,
- 520 9_
- $a DNA is a powerful tool available for forensic investigations requiring identification of species. However, it is necessary to develop and validate methods able to produce results in degraded and or low quality DNA samples with the high standards obligatory in forensic research. Here, we describe a voluntary collaborative exercise to test the recently developed Species Identification by Insertions/Deletions (SPInDel) method. The SPInDel kit allows the identification of species by the generation of numeric profiles combining the lengths of six mitochondrial ribosomal RNA (rRNA) gene regions amplified in a single reaction followed by capillary electrophoresis. The exercise was organized during 2014 by a Working Commission of the Spanish and Portuguese-Speaking Working Group of the International Society for Forensic Genetics (GHEP-ISFG), created in 2013. The 24 participating laboratories from 10 countries were asked to identify the species in 11 DNA samples from previous GHEP-ISFG proficiency tests using a SPInDel primer mix and control samples of the 10 target species. A computer software was also provided to the participants to assist the analyses of the results. All samples were correctly identified by 22 of the 24 laboratories, including samples with low amounts of DNA (hair shafts) and mixtures of saliva and blood. Correct species identifications were obtained in 238 of the 241 (98.8%) reported SPInDel profiles. Two laboratories were responsible for the three cases of misclassifications. The SPInDel was efficient in the identification of species in mixtures considering that only a single laboratory failed to detect a mixture in one sample. This result suggests that SPInDel is a valid method for mixture analyses without the need for DNA sequencing, with the advantage of identifying more than one species in a single reaction. The low frequency of wrong (5.0%) and missing (2.1%) alleles did not interfere with the correct species identification, which demonstrated the advantage of using a method based on the analysis of multiple loci. Overall, the SPInDel method was easily implemented by laboratories using different genotyping platforms, the interpretation of results was straightforward and the SPInDel software was used without any problems. The results of this collaborative exercise indicate that the SPInDel method can be applied successfully in forensic casework investigations.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a kooperační chování $7 D003299
- 650 12
- $a elektroforéza kapilární $7 D019075
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a laboratoře $7 D007753
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 12
- $a multiplexová polymerázová řetězová reakce $7 D060885
- 650 _2
- $a RNA ribozomální $x genetika $7 D012335
- 650 12
- $a druhová specificita $7 D013045
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Pereira, Rui $u Instituto de Investigação e Inovação em Saúde, I3S, Universidade do Porto, Portugal; Instituto de Patologia e Imunologia Molecular da Universidade do Porto (IPATIMUP), Porto, Portugal.
- 700 1_
- $a Prieto, Lourdes $u Grupo de Medicina Xenómica. Instituto de Ciencias Forenses. Universidade de Santiago de Compostela, Spain.
- 700 1_
- $a Aler, Mercedes $u Sección de genética forense y criminalistica, Instituto de Medicina Legal y Ciencias Forenses de Valencia, Valencia, Spain. $7 gn_A_00003836
- 700 1_
- $a Amaral, Cesar R L $u Laboratório de Diagnóstico por DNA, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil. $7 gn_A_00005262
- 700 1_
- $a Arévalo, Cristina $u Laboratorio Biología-ADN, Comisaría General de Policía Científica, Instituto Universitario de Investigación en Ciencias Policiales (Universidad de Alcalá de Henares), Spain. $7 gn_A_00008295
- 700 1_
- $a Berardi, Gabriela $u PRICAI-Fundación Favaloro, CABA, Argentina.
- 700 1_
- $a Di Rocco, Florencia $u Laboratorio de Genética Molecular, Instituto Multidisciplinario de Biología Celular, CIC-CONICET-UNLP,La Plata, Argentina.
- 700 1_
- $a Caputo, Mariela $u Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Microbiología, Inmunología y Biotecnología, Cátedra de Genética Forense y Servicio de Huellas Digitales Genéticas, Junín 956 & CONICET - Consejo Nacional de Investigaciones Científicas y Tecnológicas, C1033AAJ, Buenos Aires, Argentina.
- 700 1_
- $a Carmona, Cristian Hernandez $u Sección de Bioquímica, Departamento de Ciencias Forenses Poder Judicial de Costa Rica, Costa Rica.
- 700 1_
- $a Catelli, Laura $u Laboratorio del Equipo Argentino de Antropología Forense (EAAF) y Laboratorio de Inmunogenética y Diagnóstico Molecular (LIDMO), Córdoba, Argentina.
- 700 1_
- $a Costa, Heloísa Afonso $u Serviço de Genética e Biologia Forense, Delegação do Sul, Instituto Nacional de Medicina Legal e Ciências Forenses, I.P., Lisboa, Portugal.
- 700 1_
- $a Coufalova, Pavla $u Institute of Criminalistics Prague, Prague, Czech Republic.
- 700 1_
- $a Furfuro, Sandra $u Laboratorio de Análisis de ADN, Facultad de Ciencias Médicas - UNCuyo, Mendoza, Argentina.
- 700 1_
- $a García, Óscar $u Forensic Science Unit, Forensic Genetics Section, Basque Country Police-Ertzaintza, Erandio, Bizkaia, Spain.
- 700 1_
- $a Gaviria, Anibal $u Laboratorio de Genética Molecular, Cruz Roja Ecuatoriana - Cruz Vital, Quito, Ecuador.
- 700 1_
- $a Goios, Ana $u Instituto de Investigação e Inovação em Saúde, I3S, Universidade do Porto, Portugal; Instituto de Patologia e Imunologia Molecular da Universidade do Porto (IPATIMUP), Porto, Portugal.
- 700 1_
- $a Gómez, Juan José Builes $u Laboratorio Genes SAS, Medellín, Colombia and Instituto de Biología, Universidad de Antioquia, Medellín, Colombia.
- 700 1_
- $a Hernández, Alexis $u INTCF - Instituto Nacional de Toxicología y Ciencias Forenses, Delegación de Canarias, Santa Cruz de Tenerife, Spain.
- 700 1_
- $a Hernández, Eva Del Carmen Betancor $u Instituto de Medicina Legal de Las Palmas, Laboratorio Genética Forense, Spain.
- 700 1_
- $a Miranda, Luís $u Laboratório de Genética Aplicada, Universidade de Aveiro, Portugal.
- 700 1_
- $a Parra, David $u Servicio de Criminalística de la Guardia Civil, Departamento de Química y Medio Ambiente, Madrid, Spain.
- 700 1_
- $a Pedrosa, Susana $u Unidad de Laboratorio de Navarra de Servicios y Tecnologías, Villava, Spain.
- 700 1_
- $a Porto, Maria João Anjos $u Serviço de Genética e Biologia Forenses, Delegação do Centro, Instituto Nacional de Medicina Legal e Ciências Forenses, I.P., Coimbra, Portugal.
- 700 1_
- $a Rebelo, Maria de Lurdes $u Delegação do Norte, Instituto Nacional de Medicina Legal e Ciências Forenses, I.P. Porto, Portugal.
- 700 1_
- $a Spirito, Matteo $u Istituto di Sanità Pubblica Sezione di Medicina Legale Università Cattolica del Sacro Cuore, Roma, Italy.
- 700 1_
- $a Torres, María Del Carmen Villalobos $u Unidad de Diagnóstico Molecular, Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, México.
- 700 1_
- $a Amorim, António $u Instituto de Investigação e Inovação em Saúde, I3S, Universidade do Porto, Portugal; Instituto de Patologia e Imunologia Molecular da Universidade do Porto (IPATIMUP), Porto, Portugal; Faculty of Sciences, University of Porto, Porto, Portugal. $7 gn_A_00005710
- 700 1_
- $a Pereira, Filipe $u Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, Porto, Portugal. Electronic address: fpereirapt@gmail.com.
- 773 0_
- $w MED00166775 $t Forensic science international. Genetics $x 1878-0326 $g Roč. 28, č. - (2017), s. 219-224
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/28324847 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20171025 $b ABA008
- 991 __
- $a 20171102104855 $b ABA008
- 999 __
- $a ok $b bmc $g 1254539 $s 991973
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 28 $c - $d 219-224 $e 20170307 $i 1878-0326 $m Forensic science international. Genetics $n Forensic Sci Int Genet $x MED00166775
- LZP __
- $a Pubmed-20171025
