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Rhodamine bound maghemite as a long-term dual imaging nanoprobe of adipose tissue-derived mesenchymal stromal cells

V. Cmiel, J. Skopalik, K. Polakova, J. Solar, M. Havrdova, D. Milde, I. Justan, M. Magro, Z. Starcuk, I. Provaznik,

. 2017 ; 46 (5) : 433-444. [pub] 20161126

Language English Country Germany

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc17031267
E-resources Online Full text

NLK ProQuest Central from 1997-01-01 to 2019-01-31
Medline Complete (EBSCOhost) from 1996-11-01 to 1 year ago
Health & Medicine (ProQuest) from 1997-01-01 to 2019-01-31

Links

PubMed 27889810
DOI 10.1007/s00249-016-1187-1
Knihovny.cz E-resources

In the last few years, magnetically labeled cells have been intensively explored, and non-invasive cell tracking and magnetic manipulation methods have been tested in preclinical studies focused on cell transplantation. For clinical applications, it is desirable to know the intracellular pathway of nanoparticles, which can predict their biocompatibility with cells and the long-term imaging properties of labeled cells. Here, we quantified labeling efficiency, localization, and fluorescence properties of Rhodamine derivatized superparamagnetic maghemite nanoparticles (SAMN-R) in mesenchymal stromal cells (MSC). We investigated the stability of SAMN-R in the intracellular space during a long culture (20 days). Analyses were based on advanced confocal microscopy accompanied by atomic absorption spectroscopy (AAS) and magnetic resonance imaging. SAMN-R displayed excellent cellular uptake (24 h of labeling), and no toxicity of SAMN-R labeling was found. 83% of SAMN-R nanoparticles were localized in lysosomes, only 4.8% were found in mitochondria, and no particles were localized in the nucleus. On the basis of the MSC fluorescence measurement every 6 days, we also quantified the continual decrease of SAMN-R fluorescence in the average single MSC during 18 days. An additional set of analyses showed that the intracellular SAMN-R signal decrease was minimally caused by fluorophore degradation or nanoparticles extraction from the cells, main reason is a cell division. The fluorescence of SAMN-R nanoparticles within the cells was detectable minimally for 20 days. These observations indicate that SAMN-R nanoparticles have a potential for application in transplantation medicine.

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$a Cmiel, Vratislav $u Department of Biomedical Engineering, FEEC, Brno University of Technology, Brno, Czech Republic.
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$a In the last few years, magnetically labeled cells have been intensively explored, and non-invasive cell tracking and magnetic manipulation methods have been tested in preclinical studies focused on cell transplantation. For clinical applications, it is desirable to know the intracellular pathway of nanoparticles, which can predict their biocompatibility with cells and the long-term imaging properties of labeled cells. Here, we quantified labeling efficiency, localization, and fluorescence properties of Rhodamine derivatized superparamagnetic maghemite nanoparticles (SAMN-R) in mesenchymal stromal cells (MSC). We investigated the stability of SAMN-R in the intracellular space during a long culture (20 days). Analyses were based on advanced confocal microscopy accompanied by atomic absorption spectroscopy (AAS) and magnetic resonance imaging. SAMN-R displayed excellent cellular uptake (24 h of labeling), and no toxicity of SAMN-R labeling was found. 83% of SAMN-R nanoparticles were localized in lysosomes, only 4.8% were found in mitochondria, and no particles were localized in the nucleus. On the basis of the MSC fluorescence measurement every 6 days, we also quantified the continual decrease of SAMN-R fluorescence in the average single MSC during 18 days. An additional set of analyses showed that the intracellular SAMN-R signal decrease was minimally caused by fluorophore degradation or nanoparticles extraction from the cells, main reason is a cell division. The fluorescence of SAMN-R nanoparticles within the cells was detectable minimally for 20 days. These observations indicate that SAMN-R nanoparticles have a potential for application in transplantation medicine.
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$a Skopalik, Josef $u Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. j.skopalik@gmail.com.
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$a Polakova, Katerina $u Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Experimental Physics and Analytical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic. dr.kacka.polakova@gmail.com.
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$a Solar, Jan $u International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.
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$a Havrdova, Marketa $u Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Experimental Physics and Analytical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic.
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$a Milde, David $u Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Experimental Physics and Analytical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic.
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$a Justan, Ivan $u Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.
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$a Magro, Massimiliano $u Department of Comparative Biomedicine and Food Science, University of Padua, Padua, Italy.
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$a Starcuk, Zenon $u Institute of Scientific Instruments, The Czech Academy of Sciences, Brno, Czech Republic.
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$a Provaznik, Ivo $u Department of Biomedical Engineering, FEEC, Brno University of Technology, Brno, Czech Republic.
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