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Tracking T-cell immune reconstitution after TCRαβ/CD19-depleted hematopoietic cells transplantation in children
IV. Zvyagin, IZ. Mamedov, OV. Tatarinova, EA. Komech, EE. Kurnikova, EV. Boyakova, V. Brilliantova, LN. Shelikhova, DN. Balashov, M. Shugay, AL. Sycheva, SA. Kasatskaya, YB. Lebedev, AA. Maschan, MA. Maschan, DM. Chudakov,
Language English Country England, Great Britain
Document type Journal Article
NLK
ProQuest Central
from 2000-01-01 to 1 year ago
Open Access Digital Library
from 1997-01-01
Nursing & Allied Health Database (ProQuest)
from 2000-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 2000-01-01 to 1 year ago
Public Health Database (ProQuest)
from 2000-01-01 to 1 year ago
PubMed
27811849
DOI
10.1038/leu.2016.321
Knihovny.cz E-resources
- MeSH
- Antigens, CD19 * MeSH
- Time Factors MeSH
- Child MeSH
- Infant MeSH
- Hematologic Diseases therapy MeSH
- Humans MeSH
- Lymphocyte Depletion methods MeSH
- Adolescent MeSH
- Young Adult MeSH
- Child, Preschool MeSH
- Graft Survival * MeSH
- Receptors, Antigen, T-Cell, alpha-beta * MeSH
- T-Lymphocytes immunology MeSH
- Hematopoietic Stem Cell Transplantation methods MeSH
- Check Tag
- Child MeSH
- Infant MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Child, Preschool MeSH
- Publication type
- Journal Article MeSH
αβT-cell-depleted allogeneic hematopoietic cell transplantation holds promise for the safe and accessible therapy of both malignant and non-malignant blood disorders. Here we employed molecular barcoding normalized T-cell receptor (TCR) profiling to quantitatively track T-cell immune reconstitution after TCRαβ-/CD19-depleted transplantation in children. We demonstrate that seemingly early reconstitution of αβT-cell counts 2 months after transplantation is based on only several hundred rapidly expanded clones originating from non-depleted graft cells. In further months, frequency of these hyperexpanded clones declines, and after 1 year the observed T-cell counts and TCRβ diversity are mostly provided by the newly produced T cells. We also demonstrate that high TCRβ diversity at day 60 observed for some of the patients is determined by recipient T cells and intrathymic progenitors that survived conditioning regimen. Our results indicate that further efforts on optimization of TCRαβ-/CD19-depleted transplantation protocols should be directed toward providing more efficient T-cell defense in the first months after transplantation.
References provided by Crossref.org
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