• Je něco špatně v tomto záznamu ?

Assessment of DNA-binding affinity of cholinesterase reactivators and electrophoretic determination of their effect on topoisomerase I and II activity

J. Janockova, E. Zilecka, J. Kasparkova, V. Brabec, O. Soukup, K. Kuca, M. Kozurkova,

. 2016 ; 12 (9) : 2910-20.

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc17031589

In this paper, we describe the biochemical properties and biological activity of a series of cholinesterase reactivators (symmetrical bisquaternary xylene-linked compounds, K106-K114) with ctDNA. The interaction of the studied derivatives with ctDNA was investigated using UV-Vis, fluorescence, CD and LD spectrometry, and electrophoretic and viscometric methods. The binding constants K were estimated to be in the range 1.05 × 10(5)-5.14 × 10(6) M(-1) and the percentage of hypochromism was found to be 10.64-19.28% (from UV-Vis titration). The used methods indicate that the studied samples are groove binders. Electrophoretic methods proved that the studied compounds clearly influence calf thymus Topo I (at 5 μM concentration, except for compounds K107, K111 and K114 which were effective at higher concentrations) and human Topo II (K110 partially inhibited Topo II effects even at 5 μM concentration) activity.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17031589
003      
CZ-PrNML
005      
20171103101330.0
007      
ta
008      
171025s2016 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1039/c6mb00332j $2 doi
035    __
$a (PubMed)27412811
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Janockova, J $u Institute of Chemistry, Department of Biochemistry, Faculty of Science, P. J. Safarik University, Moyzesova 11, 040 01 Kosice, Slovak Republic. maria.kozurkova@upjs.sk and Biomedical Research Center, University Hospital Hradec Kralove, Sokolska 581, 500 05 Hradec Kralove, Czech Republic.
245    10
$a Assessment of DNA-binding affinity of cholinesterase reactivators and electrophoretic determination of their effect on topoisomerase I and II activity / $c J. Janockova, E. Zilecka, J. Kasparkova, V. Brabec, O. Soukup, K. Kuca, M. Kozurkova,
520    9_
$a In this paper, we describe the biochemical properties and biological activity of a series of cholinesterase reactivators (symmetrical bisquaternary xylene-linked compounds, K106-K114) with ctDNA. The interaction of the studied derivatives with ctDNA was investigated using UV-Vis, fluorescence, CD and LD spectrometry, and electrophoretic and viscometric methods. The binding constants K were estimated to be in the range 1.05 × 10(5)-5.14 × 10(6) M(-1) and the percentage of hypochromism was found to be 10.64-19.28% (from UV-Vis titration). The used methods indicate that the studied samples are groove binders. Electrophoretic methods proved that the studied compounds clearly influence calf thymus Topo I (at 5 μM concentration, except for compounds K107, K111 and K114 which were effective at higher concentrations) and human Topo II (K110 partially inhibited Topo II effects even at 5 μM concentration) activity.
650    _2
$a reaktivátory cholinesterasy $x chemie $x farmakologie $7 D002801
650    _2
$a cirkulární dichroismus $7 D002942
650    _2
$a DNA $x chemie $x metabolismus $7 D004247
650    _2
$a DNA-topoisomerasy I $x chemie $x metabolismus $7 D004264
650    _2
$a DNA-topoisomerasy typu II $x chemie $x metabolismus $7 D004250
650    _2
$a molekulární struktura $7 D015394
650    _2
$a denaturace nukleových kyselin $7 D009691
650    _2
$a vazba proteinů $7 D011485
650    _2
$a spektrální analýza $7 D013057
650    _2
$a viskozita $7 D014783
655    _2
$a časopisecké články $7 D016428
700    1_
$a Zilecka, E $u Institute of Chemistry, Department of Biochemistry, Faculty of Science, P. J. Safarik University, Moyzesova 11, 040 01 Kosice, Slovak Republic. maria.kozurkova@upjs.sk.
700    1_
$a Kasparkova, J $u Department of Biophysics, Faculty of Science, Palacky University, Slechtitelu 27, 783 71 Olomouc, Czech Republic.
700    1_
$a Brabec, V $u Department of Biophysics, Faculty of Science, Palacky University, Slechtitelu 27, 783 71 Olomouc, Czech Republic.
700    1_
$a Soukup, O $u Department of Toxicology and Military Pharmacy, Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic.
700    1_
$a Kuca, K $u Biomedical Research Center, University Hospital Hradec Kralove, Sokolska 581, 500 05 Hradec Kralove, Czech Republic.
700    1_
$a Kozurkova, M $u Institute of Chemistry, Department of Biochemistry, Faculty of Science, P. J. Safarik University, Moyzesova 11, 040 01 Kosice, Slovak Republic. maria.kozurkova@upjs.sk and Biomedical Research Center, University Hospital Hradec Kralove, Sokolska 581, 500 05 Hradec Kralove, Czech Republic.
773    0_
$w MED00169253 $t Molecular bioSystems $x 1742-2051 $g Roč. 12, č. 9 (2016), s. 2910-20
856    41
$u https://pubmed.ncbi.nlm.nih.gov/27412811 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20171025 $b ABA008
991    __
$a 20171103101425 $b ABA008
999    __
$a ok $b bmc $g 1255182 $s 992616
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2016 $b 12 $c 9 $d 2910-20 $i 1742-2051 $m Molecular bioSystems $n Mol Biosyst $x MED00169253
LZP    __
$a Pubmed-20171025

Najít záznam

Citační ukazatele

Možnosti archivace