-
Something wrong with this record ?
Comparison of Varroa destructor and Worker Honeybee Microbiota Within Hives Indicates Shared Bacteria
J. Hubert, M. Kamler, M. Nesvorna, O. Ledvinka, J. Kopecky, T. Erban,
Language English Country United States
Document type Comparative Study, Journal Article
NLK
ProQuest Central
from 2000-11-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2000-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 2000-11-01 to 1 year ago
- MeSH
- Biodiversity MeSH
- DNA, Bacterial genetics MeSH
- Microbiota * MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Seasons MeSH
- Sequence Analysis, DNA MeSH
- Spiroplasma classification isolation & purification MeSH
- Symbiosis MeSH
- Varroidae microbiology MeSH
- Bees microbiology parasitology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
The ectoparasitic mite Varroa destructor is a major pest of the honeybee Apis mellifera. In a previous study, bacteria were found in the guts of mites collected from winter beehive debris and were identified using Sanger sequencing of their 16S rRNA genes. In this study, community comparison and diversity analyses were performed to examine the microbiota of honeybees and mites at the population level. The microbiota of the mites and honeybees in 26 colonies in seven apiaries in Czechia was studied. Between 10 and 50 Varroa females were collected from the bottom board, and 10 worker bees were removed from the peripheral comb of the same beehive. Both bees and mites were surface sterilized. Analysis of the 16S rRNA gene libraries revealed significant differences in the Varroa and honeybee microbiota. The Varroa microbiota was less diverse than was the honeybee microbiota, and the relative abundances of bacterial taxa in the mite and bee microbiota differed. The Varroa mites, but not the honeybees, were found to be inhabited by Diplorickettsia. The relative abundance of Arsenophonus, Morganella, Spiroplasma, Enterococcus, and Pseudomonas was higher in Varroa than in honeybees, and the Diplorickettsia symbiont detected in this study is specific to Varroa mites. The results demonstrated that there are shared bacteria between Varroa and honeybee populations but that these bacteria occur in different relative proportions in the honeybee and mite bacteriomes. These results support the suggestion of bacterial transfer via mites, although only some of the transferred bacteria may be harmful.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc17031766
- 003
- CZ-PrNML
- 005
- 20201203122325.0
- 007
- ta
- 008
- 171025s2016 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s00248-016-0776-y $2 doi
- 035 __
- $a (PubMed)27129319
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Hubert, Jan, $d 1972- $u Biologically Active Substances in Crop Protection, Crop Research Institute, Drnovska 507/73, Prague 6-Ruzyne, 16106, Czechia. hubert@vurv.cz. $7 mzk2005306688
- 245 10
- $a Comparison of Varroa destructor and Worker Honeybee Microbiota Within Hives Indicates Shared Bacteria / $c J. Hubert, M. Kamler, M. Nesvorna, O. Ledvinka, J. Kopecky, T. Erban,
- 520 9_
- $a The ectoparasitic mite Varroa destructor is a major pest of the honeybee Apis mellifera. In a previous study, bacteria were found in the guts of mites collected from winter beehive debris and were identified using Sanger sequencing of their 16S rRNA genes. In this study, community comparison and diversity analyses were performed to examine the microbiota of honeybees and mites at the population level. The microbiota of the mites and honeybees in 26 colonies in seven apiaries in Czechia was studied. Between 10 and 50 Varroa females were collected from the bottom board, and 10 worker bees were removed from the peripheral comb of the same beehive. Both bees and mites were surface sterilized. Analysis of the 16S rRNA gene libraries revealed significant differences in the Varroa and honeybee microbiota. The Varroa microbiota was less diverse than was the honeybee microbiota, and the relative abundances of bacterial taxa in the mite and bee microbiota differed. The Varroa mites, but not the honeybees, were found to be inhabited by Diplorickettsia. The relative abundance of Arsenophonus, Morganella, Spiroplasma, Enterococcus, and Pseudomonas was higher in Varroa than in honeybees, and the Diplorickettsia symbiont detected in this study is specific to Varroa mites. The results demonstrated that there are shared bacteria between Varroa and honeybee populations but that these bacteria occur in different relative proportions in the honeybee and mite bacteriomes. These results support the suggestion of bacterial transfer via mites, although only some of the transferred bacteria may be harmful.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a včely $x mikrobiologie $x parazitologie $7 D001516
- 650 _2
- $a biodiverzita $7 D044822
- 650 _2
- $a DNA bakterií $x genetika $7 D004269
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 12
- $a mikrobiota $7 D064307
- 650 _2
- $a RNA ribozomální 16S $x genetika $7 D012336
- 650 _2
- $a roční období $7 D012621
- 650 _2
- $a sekvenční analýza DNA $7 D017422
- 650 _2
- $a Spiroplasma $x klasifikace $x izolace a purifikace $7 D013149
- 650 _2
- $a symbióza $7 D013559
- 650 _2
- $a Varroidae $x mikrobiologie $7 D057077
- 655 _2
- $a srovnávací studie $7 D003160
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Kamler, Martin, $d 1979- $u Bee Research Institute at Dol, Libcice nad Vltavou, Czechia. $7 xx0123249
- 700 1_
- $a Nesvorná, Marta $u Biologically Active Substances in Crop Protection, Crop Research Institute, Drnovska 507/73, Prague 6-Ruzyne, 16106, Czechia. $7 mzk2016907308
- 700 1_
- $a Ledvinka, Ondřej, $d 1982- $u Hydrological Database & Water Balance, Czech Hydrometeorological Institute, Na Sabatce 2050/17, 143 06, Prague 412, Czechia. $7 jo2014828815
- 700 1_
- $a Kopecký, Jan, $d 1966- $u Epidemiology and Ecology of Microorganisms, Crop Research Institute, Drnovska 507, Prague 6, Ruzyne, CZ, 161 06, Czechia. $7 xx0108100
- 700 1_
- $a Erban, Tomáš, $d 1978- $u Biologically Active Substances in Crop Protection, Crop Research Institute, Drnovska 507/73, Prague 6-Ruzyne, 16106, Czechia. $7 mzk2005306687
- 773 0_
- $w MED00003334 $t Microbial ecology $x 1432-184X $g Roč. 72, č. 2 (2016), s. 448-59
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/27129319 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20171025 $b ABA008
- 991 __
- $a 20201203122323 $b ABA008
- 999 __
- $a ok $b bmc $g 1255359 $s 992793
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 72 $c 2 $d 448-59 $e 20160429 $i 1432-184X $m Microbial ecology $n Microb Ecol $x MED00003334
- LZP __
- $a Pubmed-20171025
