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Purification of Natural Antibodies Against Tau Protein by Affinity Chromatography
M. Krestova, L. Hromadkova, J. Ricny,
Methods in molecular biology.
2017 ;
1643 (-) :
33-44.
Language English Country United States
Document type Journal Article
Persistent link
https://www.medvik.cz/link/bmc18010442
- MeSH
- Autoantibodies immunology isolation & purification MeSH
- Chromatography, Affinity * MeSH
- Immunosorbent Techniques MeSH
- Immunoglobulins, Intravenous MeSH
- Humans MeSH
- tau Proteins immunology MeSH
- Antibodies immunology isolation & purification MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
Natural antibodies are now widely studied for their therapeutical potential. Therefore, their isolation and subsequent characterization is desired. Here, we describe an isolation method for natural anti-tau antibodies from human plasma by utilization of affinity chromatography with epoxy-activated copolymer resin. The evalution of isolation efficiency and avidity of isolated antibodies is decribed by modified indirect ELISA assay.
References provided by Crossref.org
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