-
Je něco špatně v tomto záznamu ?
Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells
A. Rabata, A. Hampl, Z. Koledova,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
- MeSH
- buněčné kultury metody MeSH
- buněčné sféroidy cytologie MeSH
- epitelové buňky cytologie MeSH
- extracelulární matrix chemie MeSH
- kmenové buňky cytologie MeSH
- myši MeSH
- plíce cytologie MeSH
- proliferace buněk MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Lung epithelium contains distinctive subpopulations of lung stem/progenitor cells (LSPCs) that are essential for lung epithelial maintenance and repair in vivo. Hence, LSPCs are in the center of interest of lung biology due to their promising therapeutic applications. To reach this goal, proper characterization of LSPCs, understanding of their proliferation and differentiation potentials and elucidation of mechanisms that control them are necessary. Therefore, development of reliable in vitro clonogenic assays has been needed. We established lungosphere assay, an in vitro sphere-forming 3D culture assay that enables to evaluate stem/progenitor cell activity, self-renewal and differentiation capacity of LSPCs and to conveniently test the effect of various treatments on LSPCs. Here we provide a detailed description of procedures for isolation of adult mouse lung epithelial cells, their culture in non-adherent conditions to form LSPC-derived spheroids (lungospheres) and for embedding of lungospheres into 3D extracellular matrix to model processes of lung tissue maintenance in a physiologically relevant microenvironment.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18010469
- 003
- CZ-PrNML
- 005
- 20180404142043.0
- 007
- ta
- 008
- 180404s2017 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/978-1-4939-7021-6_11 $2 doi
- 035 __
- $a (PubMed)28634941
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Rabata, Anas $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic.
- 245 10
- $a Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells / $c A. Rabata, A. Hampl, Z. Koledova,
- 520 9_
- $a Lung epithelium contains distinctive subpopulations of lung stem/progenitor cells (LSPCs) that are essential for lung epithelial maintenance and repair in vivo. Hence, LSPCs are in the center of interest of lung biology due to their promising therapeutic applications. To reach this goal, proper characterization of LSPCs, understanding of their proliferation and differentiation potentials and elucidation of mechanisms that control them are necessary. Therefore, development of reliable in vitro clonogenic assays has been needed. We established lungosphere assay, an in vitro sphere-forming 3D culture assay that enables to evaluate stem/progenitor cell activity, self-renewal and differentiation capacity of LSPCs and to conveniently test the effect of various treatments on LSPCs. Here we provide a detailed description of procedures for isolation of adult mouse lung epithelial cells, their culture in non-adherent conditions to form LSPC-derived spheroids (lungospheres) and for embedding of lungospheres into 3D extracellular matrix to model processes of lung tissue maintenance in a physiologically relevant microenvironment.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a buněčné kultury $x metody $7 D018929
- 650 _2
- $a proliferace buněk $7 D049109
- 650 _2
- $a epitelové buňky $x cytologie $7 D004847
- 650 _2
- $a extracelulární matrix $x chemie $7 D005109
- 650 _2
- $a plíce $x cytologie $7 D008168
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a buněčné sféroidy $x cytologie $7 D018874
- 650 _2
- $a kmenové buňky $x cytologie $7 D013234
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Hampl, Ales $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic. International Clinical Research Center - Center of Biomolecular and Cellular Engineering, St. Anne's University Hospital Brno, Brno, Czech Republic.
- 700 1_
- $a Koledova, Zuzana $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic. koledova@med.muni.cz.
- 773 0_
- $w MED00180389 $t Methods in molecular biology (Clifton, N.J.) $x 1940-6029 $g Roč. 1612, č. - (2017), s. 149-165
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/28634941 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20180404 $b ABA008
- 991 __
- $a 20180404142122 $b ABA008
- 999 __
- $a ok $b bmc $g 1287954 $s 1007281
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 1612 $c - $d 149-165 $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
- LZP __
- $a Pubmed-20180404
