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Potential Involvement of SCF-Complex in Zygotic Genome Activation During Early Bovine Embryo Development
V. Benesova, V. Kinterova, J. Kanka, T. Toralova,
Methods in molecular biology.
2017 ;
1605 (-) :
245-257.
Language English Country United States
Document type Journal Article
Persistent link
https://www.medvik.cz/link/bmc18010615
- MeSH
- Transcriptional Activation MeSH
- Embryonic Development * MeSH
- SKP Cullin F-Box Protein Ligases metabolism MeSH
- Proteolysis MeSH
- Cattle MeSH
- Gene Expression Profiling MeSH
- Ubiquitin-Protein Ligases metabolism MeSH
- Gene Expression Regulation, Developmental MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Proper timing of degradation of maternal protein reserves is important for early embryonic development. The major modification that triggers proteins to degradation is ubiquitination, mediated by ubiquitin-proteolytic system. We focus here on Skp 1-Cul 1-F-box complex (SCF-complex), E3 ubiquitin-ligase, a part of ubiquitin-proteolytic system, which transfer ubiquitin to the substrate protein. We describe in this chapter the methods for the characterization of the expression profile of mRNA and protein of invariant members of SCF-complex and for the definition of SCF-complex activity.
References provided by Crossref.org
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