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Brassinosteroid Biosynthesis Is Modulated via a Transcription Factor Cascade of COG1, PIF4, and PIF5

Z. Wei, T. Yuan, D. Tarkowská, J. Kim, HG. Nam, O. Novák, K. He, X. Gou, J. Li,

. 2017 ; 174 (2) : 1260-1273. [pub] 20170424

Language English Country United States

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc18010647

Brassinosteroids (BRs) are essential phytohormones regulating various developmental and physiological processes during normal growth and development.cog1-3D(cogwheel1-3D) was identified as an activation-tagged genetic modifier ofbri1-5, an intermediate BR receptor mutant in Arabidopsis (Arabidopsis thaliana).COG1encodes a Dof-type transcription factor found previously to act as a negative regulator of the phytochrome signaling pathway.cog1-3Dsingle mutants show an elongated hypocotyl phenotype under light conditions. A loss-of-function mutant or inducible expression of a dominant negative form ofCOG1in the wild type results in an opposite phenotype. A BR profile assay indicated that BR levels are elevated incog1-3Dseedlings. Quantitative reverse transcription-polymerase chain reaction analyses showed that several key BR biosynthetic genes are significantly up-regulated incog1-3Dcompared with those of the wild type. Two basic helix-loop-helix transcription factors,PIF4andPIF5, were found to be transcriptionally up-regulated incog1-3DGenetic analysis indicated that PIF4 and PIF5 were required for COG1 to promote BR biosynthesis and hypocotyl elongation. Chromatin immunoprecipitation and electrophoretic mobility shift assays indicated that COG1 binds to the promoter regions ofPIF4andPIF5, and PIF4 and PIF5 bind to the promoter regions of key BR biosynthetic genes, such asDWF4andBR6ox2, to directly promote their expression. These results demonstrated that COG1 regulates BR biosynthesis via up-regulating the transcription ofPIF4andPIF5.

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$a Wei, Zhuoyun $u Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China (Z.W., K.H., X.G., J.L.). Department of Plant Biology and Microbiology, University of Oklahoma, Norman, Oklahoma 73019 (T.Y.). Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University and Institute of Experimental Botany, Academy of Sciences of the Czech Republic, CZ-78371 Olomouc, Czech Republic (D.T., O.N.). Center for Plant Aging Research, Institute for Basic Science, Daegu 42988, Republic of Korea (J.K., H.G.N.); and. Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology, Daegu 42988, Republic of Korea (H.G.N.).
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$a Brassinosteroid Biosynthesis Is Modulated via a Transcription Factor Cascade of COG1, PIF4, and PIF5 / $c Z. Wei, T. Yuan, D. Tarkowská, J. Kim, HG. Nam, O. Novák, K. He, X. Gou, J. Li,
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$a Brassinosteroids (BRs) are essential phytohormones regulating various developmental and physiological processes during normal growth and development.cog1-3D(cogwheel1-3D) was identified as an activation-tagged genetic modifier ofbri1-5, an intermediate BR receptor mutant in Arabidopsis (Arabidopsis thaliana).COG1encodes a Dof-type transcription factor found previously to act as a negative regulator of the phytochrome signaling pathway.cog1-3Dsingle mutants show an elongated hypocotyl phenotype under light conditions. A loss-of-function mutant or inducible expression of a dominant negative form ofCOG1in the wild type results in an opposite phenotype. A BR profile assay indicated that BR levels are elevated incog1-3Dseedlings. Quantitative reverse transcription-polymerase chain reaction analyses showed that several key BR biosynthetic genes are significantly up-regulated incog1-3Dcompared with those of the wild type. Two basic helix-loop-helix transcription factors,PIF4andPIF5, were found to be transcriptionally up-regulated incog1-3DGenetic analysis indicated that PIF4 and PIF5 were required for COG1 to promote BR biosynthesis and hypocotyl elongation. Chromatin immunoprecipitation and electrophoretic mobility shift assays indicated that COG1 binds to the promoter regions ofPIF4andPIF5, and PIF4 and PIF5 bind to the promoter regions of key BR biosynthetic genes, such asDWF4andBR6ox2, to directly promote their expression. These results demonstrated that COG1 regulates BR biosynthesis via up-regulating the transcription ofPIF4andPIF5.
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$a Nam, Hong Gil $u Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China (Z.W., K.H., X.G., J.L.). Department of Plant Biology and Microbiology, University of Oklahoma, Norman, Oklahoma 73019 (T.Y.). Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University and Institute of Experimental Botany, Academy of Sciences of the Czech Republic, CZ-78371 Olomouc, Czech Republic (D.T., O.N.). Center for Plant Aging Research, Institute for Basic Science, Daegu 42988, Republic of Korea (J.K., H.G.N.); and. Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology, Daegu 42988, Republic of Korea (H.G.N.).
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