-
Something wrong with this record ?
Steroid free immunosuppression is associated with enhanced Th1 transcripts in kidney transplantation
P. Hruba, I. Tycova, E. Krepsova, E. Girmanova, A. Sekerkova, J. Slatinska, I. Striz, E. Honsova, O. Viklicky,
Language English Country Netherlands
Document type Journal Article, Multicenter Study, Observational Study, Randomized Controlled Trial, Research Support, Non-U.S. Gov't
Grant support
NV15-26865A
MZ0
CEP Register
- MeSH
- Killer Cells, Natural immunology metabolism MeSH
- Adult MeSH
- Immunosuppression Therapy methods MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Prospective Studies MeSH
- Gene Expression Regulation * MeSH
- Aged MeSH
- Th1 Cells immunology metabolism MeSH
- Kidney Transplantation * MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Multicenter Study MeSH
- Observational Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Randomized Controlled Trial MeSH
BACKGROUND: Steroid avoidance in immunosuppression in kidney transplantation offers several metabolic advantages, however it is associated with higher early acute rejection rate. Cellular and molecular mechanisms of this phenomenon remain poorly understood. METHODS: In this single center observational study, low-risk kidney transplant recipients randomized into large multicenter prospective ADVANCE trial with steroid avoidance/early withdrawal and center standard of care treated patients were monitored for 12months. The expressions of 28 transcripts, associated with alloimmune response and operational tolerance, were evaluated in the peripheral blood using RT-qPCR at 0, 7, 14, 90 and 365 postoperative days (POD) and in the protocol graft biopsy at 3months while lymphocyte subpopulations were analyzed by flow-cytometry within the follow-up. RESULTS: Both steroid avoidance and withdrawal regimens were associated with significantly higher granzyme B (GZMB) transcript at POD 14 and perforin 1 (PRF1) transcript at POD 7. The higher interleukin 2 (IL-2) expression at POD 7 was detected only in the steroid avoidance group. Initial steroids decreased the expression SH2D1B transcript at POD14 and there were no further differences in other operational tolerance transcripts among groups. The statistically significant decrease in absolute numbers of peripheral NK cells in the first 14days was observed in the standard of care group only. There were no differences in analyzed intrarenal transcripts in 3-month biopsies among groups. CONCLUSIONS: The enhanced expression of some of Th1 associated transcripts and limited effects on NK cells of steroid avoidance immunosuppression suggest higher susceptibility for early acute rejection.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18010706
- 003
- CZ-PrNML
- 005
- 20201016161802.0
- 007
- ta
- 008
- 180404s2017 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.trim.2017.03.001 $2 doi
- 035 __
- $a (PubMed)28366698
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Hruba, Petra $u Transplant Laboratory, Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
- 245 10
- $a Steroid free immunosuppression is associated with enhanced Th1 transcripts in kidney transplantation / $c P. Hruba, I. Tycova, E. Krepsova, E. Girmanova, A. Sekerkova, J. Slatinska, I. Striz, E. Honsova, O. Viklicky,
- 520 9_
- $a BACKGROUND: Steroid avoidance in immunosuppression in kidney transplantation offers several metabolic advantages, however it is associated with higher early acute rejection rate. Cellular and molecular mechanisms of this phenomenon remain poorly understood. METHODS: In this single center observational study, low-risk kidney transplant recipients randomized into large multicenter prospective ADVANCE trial with steroid avoidance/early withdrawal and center standard of care treated patients were monitored for 12months. The expressions of 28 transcripts, associated with alloimmune response and operational tolerance, were evaluated in the peripheral blood using RT-qPCR at 0, 7, 14, 90 and 365 postoperative days (POD) and in the protocol graft biopsy at 3months while lymphocyte subpopulations were analyzed by flow-cytometry within the follow-up. RESULTS: Both steroid avoidance and withdrawal regimens were associated with significantly higher granzyme B (GZMB) transcript at POD 14 and perforin 1 (PRF1) transcript at POD 7. The higher interleukin 2 (IL-2) expression at POD 7 was detected only in the steroid avoidance group. Initial steroids decreased the expression SH2D1B transcript at POD14 and there were no further differences in other operational tolerance transcripts among groups. The statistically significant decrease in absolute numbers of peripheral NK cells in the first 14days was observed in the standard of care group only. There were no differences in analyzed intrarenal transcripts in 3-month biopsies among groups. CONCLUSIONS: The enhanced expression of some of Th1 associated transcripts and limited effects on NK cells of steroid avoidance immunosuppression suggest higher susceptibility for early acute rejection.
- 650 _2
- $a mladiství $7 D000293
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a senioři $7 D000368
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 12
- $a regulace genové exprese $7 D005786
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a imunosupresivní léčba $x metody $7 D007165
- 650 12
- $a transplantace ledvin $7 D016030
- 650 _2
- $a buňky NK $x imunologie $x metabolismus $7 D007694
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a prospektivní studie $7 D011446
- 650 _2
- $a Th1 buňky $x imunologie $x metabolismus $7 D018417
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a multicentrická studie $7 D016448
- 655 _2
- $a pozorovací studie $7 D064888
- 655 _2
- $a randomizované kontrolované studie $7 D016449
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Tycova, Irena $u Transplant Laboratory, Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
- 700 1_
- $a Krepsova, Eva $u Transplant Laboratory, Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
- 700 1_
- $a Girmanova, Eva $u Transplant Laboratory, Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
- 700 1_
- $a Sekerkova, Alena $u Department of Clinical and Transplant Immunology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
- 700 1_
- $a Slatinska, Janka $u Department of Nephrology, Transplant Center, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
- 700 1_
- $a Striz, Ilja $u Department of Clinical and Transplant Immunology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
- 700 1_
- $a Honsova, Eva $u Department of Clinical and Transplant Pathology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
- 700 1_
- $a Viklicky, Ondrej $u Transplant Laboratory, Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic; Department of Nephrology, Transplant Center, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. Electronic address: ondrej.viklicky@ikem.cz.
- 773 0_
- $w MED00006022 $t Transplant immunology $x 1878-5492 $g Roč. 42, č. - (2017), s. 18-23
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/28366698 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20180404 $b ABA008
- 991 __
- $a 20201016161800 $b ABA008
- 999 __
- $a ok $b bmc $g 1288191 $s 1007518
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 42 $c - $d 18-23 $e 20170331 $i 1878-5492 $m Transplant immunology $n Transpl Immunol $x MED00006022
- GRA __
- $a NV15-26865A $p MZ0
- LZP __
- $a Pubmed-20180404
