-
Je něco špatně v tomto záznamu ?
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
V. Ledvina, E. Janečková, E. Matalová, K. Klepárník,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
NLK
ProQuest Central
od 2011-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2003-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2011-01-01 do Před 1 rokem
- MeSH
- analýza jednotlivých buněk přístrojové vybavení metody MeSH
- apoptóza * MeSH
- design vybavení MeSH
- enzymatické testy přístrojové vybavení metody MeSH
- kaspasa 3 analýza metabolismus MeSH
- kaspasa 7 analýza metabolismus MeSH
- kultivované buňky MeSH
- luminiscenční měření přístrojové vybavení metody MeSH
- myši MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Analysing the chemical content of individual cells has already been proven to reveal unique information on various biological processes. Single-cell analysis provides more accurate and reliable results for biology and medicine than analyses of extracts from cell populations, where a natural heterogeneity is averaged. To meet the requirements in the research of important biologically active molecules, such as caspases, we have developed a miniaturized device for simultaneous analyses of individual cells. A stainless steel body with a carousel holder enables high-sensitivity parallel detections in eight microvials. The holder is mounted in front of a photomultiplier tube with cooled photocathode working in photon counting mode. The detection of active caspase-3/7, central effector caspases in apoptosis, in single cells is based on the bioluminescence chemistry commercially available as Caspase-Glo®3/7 reagent developed by Promega. Individual cells were captured from a culture medium under microscope and transferred by micromanipulator into detection microvial filled with the reagent. As a result of testing, the limits of detection and quantification were determined to be 0.27/0.86 of active caspase-3/7 content in an average apoptotic cell and 0.46/2.92 for non-apoptotic cells. Application potential of this technology in laboratory diagnostics and related medical research is discussed. Graphical abstract Miniaturized device for simultaneous analyses of individual cells.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18010986
- 003
- CZ-PrNML
- 005
- 20180404142444.0
- 007
- ta
- 008
- 180404s2017 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s00216-016-9998-6 $2 doi
- 035 __
- $a (PubMed)27757513
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Ledvina, Vojtěch $u Institute of Analytical Chemistry, v.v.i., Czech Academy of Sciences, Veveří 97, 60200, Brno, Czech Republic. Faculty of Science, Masaryk University, Kotlářská 267/2, 61137, Brno, Czech Republic.
- 245 10
- $a Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells / $c V. Ledvina, E. Janečková, E. Matalová, K. Klepárník,
- 520 9_
- $a Analysing the chemical content of individual cells has already been proven to reveal unique information on various biological processes. Single-cell analysis provides more accurate and reliable results for biology and medicine than analyses of extracts from cell populations, where a natural heterogeneity is averaged. To meet the requirements in the research of important biologically active molecules, such as caspases, we have developed a miniaturized device for simultaneous analyses of individual cells. A stainless steel body with a carousel holder enables high-sensitivity parallel detections in eight microvials. The holder is mounted in front of a photomultiplier tube with cooled photocathode working in photon counting mode. The detection of active caspase-3/7, central effector caspases in apoptosis, in single cells is based on the bioluminescence chemistry commercially available as Caspase-Glo®3/7 reagent developed by Promega. Individual cells were captured from a culture medium under microscope and transferred by micromanipulator into detection microvial filled with the reagent. As a result of testing, the limits of detection and quantification were determined to be 0.27/0.86 of active caspase-3/7 content in an average apoptotic cell and 0.46/2.92 for non-apoptotic cells. Application potential of this technology in laboratory diagnostics and related medical research is discussed. Graphical abstract Miniaturized device for simultaneous analyses of individual cells.
- 650 _2
- $a zvířata $7 D000818
- 650 12
- $a apoptóza $7 D017209
- 650 _2
- $a kaspasa 3 $x analýza $x metabolismus $7 D053148
- 650 _2
- $a kaspasa 7 $x analýza $x metabolismus $7 D053179
- 650 _2
- $a kultivované buňky $7 D002478
- 650 _2
- $a enzymatické testy $x přístrojové vybavení $x metody $7 D057075
- 650 _2
- $a design vybavení $7 D004867
- 650 _2
- $a luminiscenční měření $x přístrojové vybavení $x metody $7 D008163
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a analýza jednotlivých buněk $x přístrojové vybavení $x metody $7 D059010
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Janečková, Eva $u Faculty of Science, Masaryk University, Kotlářská 267/2, 61137, Brno, Czech Republic. Institute of Animal Physiology and Genetics, v.v.i., Czech Academy of Sciences, Veveří 97, 60200, Brno, Czech Republic.
- 700 1_
- $a Matalová, Eva $u Institute of Animal Physiology and Genetics, v.v.i., Czech Academy of Sciences, Veveří 97, 60200, Brno, Czech Republic. Department of Physiology, University of Veterinary and Pharmaceutical Sciences, Palackého 1/3, 61242, Brno, Czech Republic.
- 700 1_
- $a Klepárník, Karel $u Institute of Analytical Chemistry, v.v.i., Czech Academy of Sciences, Veveří 97, 60200, Brno, Czech Republic. kleparnik@iach.cz.
- 773 0_
- $w MED00006638 $t Analytical and bioanalytical chemistry $x 1618-2650 $g Roč. 409, č. 1 (2017), s. 269-274
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/27757513 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20180404 $b ABA008
- 991 __
- $a 20180404142523 $b ABA008
- 999 __
- $a ok $b bmc $g 1288471 $s 1007798
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 409 $c 1 $d 269-274 $e 20161018 $i 1618-2650 $m Analytical and bioanalytical chemistry $n Anal Bioanal Chem $x MED00006638
- LZP __
- $a Pubmed-20180404
