Clinical yeast isolates belonging to Candida pelliculosa, Candida utilis and Candida fabianii are difficult to distinguish in a routine mycology laboratory using common biochemical tests. The aims of this study were to determine the prevalence of C. pelliculosa, C. utilis and C. fabianii in clinical samples and to compare their minimum inhibitory concentrations (MICs) to systemic antifungals. Two hundred and forty-eight clinical yeast isolates obtained from eight large hospitals in the Czech Republic were included in this study. Identification was performed biochemically using ID 32C kit and by MALDI-TOF MS. MICs were determined using colorimetric broth dilution Sensititre YeastOne panels. From a total number of 248 isolates, 175 were identified as C. pelliculosa and 73 as C. utilis using the biochemical kit. In contrast, MALDI-TOF MS identified 222 isolates as C. fabianii, 20 as C. pelliculosa and 6 as C. utilis. The highest mean MICs were found in C. fabianii and, regardless of the studied species, in isolates from blood cultures and central venous catheters. MALDI-TOF MS revealed C. fabianii to be most prevalent in clinical samples as compared with the other studied species. Higher MIC values in C. fabianii support the importance of correct identification of this species.

Cellular and Molecular Immunology Research Unit Department of Medical Technology Faculty of Allied Health Science Naresuan University Phitsanulok Thailand

Department of Clinical Microbiology Faculty of Medicine and University Hospital Charles University Hradec Kralove Czech Republic

Department of Clinical Microbiology University Hospital Brno Brno Czech Republic

Department of Medical Microbiology 2nd Faculty of Medicine Charles University Prague and Motol University Hospital Prague Czech Republic

Department of Microbiology Faculty of Medicine and Dentistry Palacky University Olomouc and University Hospital Olomouc Olomouc Czech Republic

Department of Microbiology Faculty of Medicine Masaryk University Brno Czech Republic

Laboratory of Clinical Mycology Public Health Institute Ostrava Ostrava Czech Republic

Laboratory of Parasitology and Mycology Ceske Budejovice Hospital Ceske Budejovice Czech Republic

Mycology Laboratory Clinical Microbiology and ATB Centre Institute of Medical Biochemistry and Laboratory Diagnostics of the General University Hospital and of the 1st Faculty of Medicine of Charles University Prague Prague Czech Republic

Research and Development Department GeneProof a s Brno Czech Republic

State Veterinary Institute Olomouc Olomouc Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc18011308

003      

CZ-PrNML

005      

20200907121500.0

007      

ta

008      

180404s2016 gw f 000 0|eng||

009      

AR

024    7_

$a 10.1111/myc.12454 $2 doi

035    __

$a (PubMed)26763103

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a gw

100    1_

$a Svobodova, L $u Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Olomouc, Czech Republic.

245    10

$a High frequency of Candida fabianii among clinical isolates biochemically identified as Candida pelliculosa and Candida utilis / $c L. Svobodova, D. Bednarova, F. Ruzicka, V. Chrenkova, R. Dobias, N. Mallatova, V. Buchta, I. Kocmanova, P. Olisarova, N. Stromerova, Y. Thongsri, P. Hamal,

520    9_

$a Clinical yeast isolates belonging to Candida pelliculosa, Candida utilis and Candida fabianii are difficult to distinguish in a routine mycology laboratory using common biochemical tests. The aims of this study were to determine the prevalence of C. pelliculosa, C. utilis and C. fabianii in clinical samples and to compare their minimum inhibitory concentrations (MICs) to systemic antifungals. Two hundred and forty-eight clinical yeast isolates obtained from eight large hospitals in the Czech Republic were included in this study. Identification was performed biochemically using ID 32C kit and by MALDI-TOF MS. MICs were determined using colorimetric broth dilution Sensititre YeastOne panels. From a total number of 248 isolates, 175 were identified as C. pelliculosa and 73 as C. utilis using the biochemical kit. In contrast, MALDI-TOF MS identified 222 isolates as C. fabianii, 20 as C. pelliculosa and 6 as C. utilis. The highest mean MICs were found in C. fabianii and, regardless of the studied species, in isolates from blood cultures and central venous catheters. MALDI-TOF MS revealed C. fabianii to be most prevalent in clinical samples as compared with the other studied species. Higher MIC values in C. fabianii support the importance of correct identification of this species.

650    _2

$a mladiství $7 D000293

650    _2

$a dospělí $7 D000328

650    _2

$a senioři $7 D000368

650    _2

$a senioři nad 80 let $7 D000369

650    _2

$a antifungální látky $x farmakologie $7 D000935

650    _2

$a Candida $x klasifikace $x izolace a purifikace $7 D002175

650    _2

$a kandidóza $x epidemiologie $x mikrobiologie $7 D002177

650    _2

$a dítě $7 D002648

650    _2

$a předškolní dítě $7 D002675

650    _2

$a Česká republika $x epidemiologie $7 D018153

650    _2

$a ženské pohlaví $7 D005260

650    _2

$a nemocnice $7 D006761

650    _2

$a lidé $7 D006801

650    _2

$a kojenec $7 D007223

650    _2

$a novorozenec $7 D007231

650    _2

$a mužské pohlaví $7 D008297

650    _2

$a mikrobiální testy citlivosti $7 D008826

650    _2

$a lidé středního věku $7 D008875

650    _2

$a mykologické určovací techniky $7 D016533

650    _2

$a prevalence $7 D015995

650    _2

$a prospektivní studie $7 D011446

650    _2

$a mladý dospělý $7 D055815

655    _2

$a časopisecké články $7 D016428

700    1_

$a Bednarova, D $u Research & Development Department, GeneProof a.s., Brno, Czech Republic.

700    1_

$a Ruzicka, F $u Department of Microbiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

700    1_

$a Chrenková, Vanda $u Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, Prague, Czech Republic. $7 xx0159343

700    1_

$a Dobias, R $u Laboratory of Clinical Mycology, Public Health Institute Ostrava, Ostrava, Czech Republic.

700    1_

$a Mallatova, N $u Laboratory of Parasitology and Mycology, Ceske Budejovice Hospital, Ceske Budejovice, Czech Republic.

700    1_

$a Buchta, V $u Department of Clinical Microbiology, Faculty of Medicine and University Hospital, Charles University, Hradec Kralove, Czech Republic.

700    1_

$a Kocmanova, I $u Department of Clinical Microbiology, University Hospital Brno, Brno, Czech Republic.

700    1_

$a Olisarova, P $u Mycology Laboratory, Clinical Microbiology and ATB Centre, Institute of Medical Biochemistry and Laboratory Diagnostics of the General University Hospital and of the First Faculty of Medicine of Charles University in Prague, Prague, Czech Republic.

700    1_

$a Stromerova, N $u State Veterinary Institute Olomouc, Olomouc, Czech Republic.

700    1_

$a Thongsri, Y $u Cellular and Molecular Immunology Research Unit (CMIRU), Department of Medical Technology, Faculty of Allied Health Science, Naresuan University, Phitsanulok, Thailand.

700    1_

$a Hamal, P $u Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Olomouc, Czech Republic.

773    0_

$w MED00010272 $t Mycoses $x 1439-0507 $g Roč. 59, č. 4 (2016), s. 241-246

856    41

$u https://pubmed.ncbi.nlm.nih.gov/26763103 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20180404 $b ABA008

991    __

$a 20200907121500 $b ABA008

999    __

$a ok $b bmc $g 1288793 $s 1008120

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2016 $b 59 $c 4 $d 241-246 $e 20160113 $i 1439-0507 $m Mycoses $n Mycoses $x MED00010272

LZP    __

$a Pubmed-20180404