-
Something wrong with this record ?
Passive Tumor Targeting of Polymer Therapeutics: In Vivo Imaging of Both the Polymer Carrier and the Enzymatically Cleavable Drug Model
R. Pola, AK. Heinrich, T. Mueller, L. Kostka, K. Mäder, M. Pechar, T. Etrych,
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Acrylamides * chemistry pharmacokinetics pharmacology MeSH
- Fluorescent Dyes * chemistry pharmacokinetics pharmacology MeSH
- Colorectal Neoplasms * drug therapy metabolism pathology MeSH
- Humans MeSH
- Mice, Nude MeSH
- Mice MeSH
- Drug Carriers * chemistry pharmacokinetics pharmacology MeSH
- Xenograft Model Antitumor Assays MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The enzymatic release of a model drug from a polymer carrier inside a tumor using multispectral optical imaging in vivo in nude mice bearing colorectal carcinomas HT-29 and DLD-1 is demonstrated. Much higher release rate in vivo from a linear (30 kDa) (N-2-hydroxypropyl)methacrylamide-based polymer compared with a high molecular weight branched (170 kDa) polymer conjugate is observed, probably due to steric hindrance of the cleavable spacer of the latter polymer to proteolytic enzymes. There is no significant difference in the relative biodistribution of the two polymers, but the branched polymer circulates much longer. Both polymers are labeled with two different fluorophores. Dyomics-676 as a drug model is attached to the polymer via an enzymatically cleavable Gly-Phe-Leu-Gly spacer; Dyomics 782 is bound to the same polymer via a nondegradable amide bond, enabling the tracking of the polymer carrier after i.v. application to mice.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18017029
- 003
- CZ-PrNML
- 005
- 20180521091300.0
- 007
- ta
- 008
- 180515s2016 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1002/mabi.201600273 $2 doi
- 035 __
- $a (PubMed)27654467
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Pola, Robert $u Department of Biomedical Polymers, Institute of Macromolecular Chemistry AS CR, Heyrovsky Sq. 2, 162 06, Prague, Czech Republic.
- 245 10
- $a Passive Tumor Targeting of Polymer Therapeutics: In Vivo Imaging of Both the Polymer Carrier and the Enzymatically Cleavable Drug Model / $c R. Pola, AK. Heinrich, T. Mueller, L. Kostka, K. Mäder, M. Pechar, T. Etrych,
- 520 9_
- $a The enzymatic release of a model drug from a polymer carrier inside a tumor using multispectral optical imaging in vivo in nude mice bearing colorectal carcinomas HT-29 and DLD-1 is demonstrated. Much higher release rate in vivo from a linear (30 kDa) (N-2-hydroxypropyl)methacrylamide-based polymer compared with a high molecular weight branched (170 kDa) polymer conjugate is observed, probably due to steric hindrance of the cleavable spacer of the latter polymer to proteolytic enzymes. There is no significant difference in the relative biodistribution of the two polymers, but the branched polymer circulates much longer. Both polymers are labeled with two different fluorophores. Dyomics-676 as a drug model is attached to the polymer via an enzymatically cleavable Gly-Phe-Leu-Gly spacer; Dyomics 782 is bound to the same polymer via a nondegradable amide bond, enabling the tracking of the polymer carrier after i.v. application to mice.
- 650 12
- $a akrylamidy $x chemie $x farmakokinetika $x farmakologie $7 D000178
- 650 _2
- $a zvířata $7 D000818
- 650 12
- $a kolorektální nádory $x farmakoterapie $x metabolismus $x patologie $7 D015179
- 650 12
- $a nosiče léků $x chemie $x farmakokinetika $x farmakologie $7 D004337
- 650 12
- $a fluorescenční barviva $x chemie $x farmakokinetika $x farmakologie $7 D005456
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a myši nahé $7 D008819
- 650 _2
- $a xenogenní modely - testy protinádorové aktivity $7 D023041
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Heinrich, Anne-Kathrin $u Department of Pharmacy, Pharmaceutical Technology, and Biopharmacy, Martin Luther University Halle-Wittenberg, 06120, Halle, Germany.
- 700 1_
- $a Mueller, Thomas $u Department of Internal Medicine IV, Oncology/Hematology, Martin Luther University Halle-Wittenberg, 06120, Halle, Germany.
- 700 1_
- $a Kostka, Libor $u Department of Biomedical Polymers, Institute of Macromolecular Chemistry AS CR, Heyrovsky Sq. 2, 162 06, Prague, Czech Republic.
- 700 1_
- $a Mäder, Karsten $u Department of Pharmacy, Pharmaceutical Technology, and Biopharmacy, Martin Luther University Halle-Wittenberg, 06120, Halle, Germany.
- 700 1_
- $a Pechar, Michal $u Department of Biomedical Polymers, Institute of Macromolecular Chemistry AS CR, Heyrovsky Sq. 2, 162 06, Prague, Czech Republic.
- 700 1_
- $a Etrych, Tomas $u Department of Biomedical Polymers, Institute of Macromolecular Chemistry AS CR, Heyrovsky Sq. 2, 162 06, Prague, Czech Republic.
- 773 0_
- $w MED00006593 $t Macromolecular bioscience $x 1616-5195 $g Roč. 16, č. 11 (2016), s. 1577-1582
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/27654467 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20180515 $b ABA008
- 991 __
- $a 20180521091442 $b ABA008
- 999 __
- $a ok $b bmc $g 1300653 $s 1013869
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 16 $c 11 $d 1577-1582 $e 20160922 $i 1616-5195 $m Macromolecular bioscience $n Macromol Biosci $x MED00006593
- LZP __
- $a Pubmed-20180515
