The transduction mediated by bacteriophages is considered to be one of the primary driving forces in horizontal gene transfer in staphylococci, which is crucial to their adaptation and successful evolution. For a transduction to be effective, it is generally accepted that the recipient strain should be susceptible to the transducing phage. In this study, we demonstrate that the plasmid DNAs are effectively transduced into the recipient Staphylococcus aureus strains in spite of their insensitivity to the lytic action of the transducing phage, provided that these phages adsorb effectively to the bacterial cells. The tetracycline and penicillinase plasmids were transduced to insensitive laboratory and clinical strains by bacteriophages ϕ29, ϕ52A and ϕ80α as well as by prophage ϕ53 and naturally occurring prophages induced from donor lysogenic strains. Comparable frequencies of transduction were achieved in both phage-sensitive and phage-insensitive recipient strains. We have demonstrated that such mechanisms as the restriction of DNA and lysogenic immunity which are responsible for insensitivity of cells to phages may not be a barrier to the transfer, maintenance and effective spread of plasmids to a wider range of potential recipients in the staphylococcal population.

Department of Experimental Biology Faculty of Science Masaryk University Kotlářská 2 CZ 611 37 Brno Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc18017056

003      

CZ-PrNML

005      

20180521090627.0

007      

ta

008      

180515s2016 xxk f 000 0|eng||

009      

AR

024    7_

$a 10.1093/femsle/fnw211 $2 doi

035    __

$a (PubMed)27609232

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxk

100    1_

$a Mašlaňová, Ivana $u Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, CZ-611 37 Brno, Czech Republic iva.maslanova@gmail.com.

245    10

$a Efficient plasmid transduction to Staphylococcus aureus strains insensitive to the lytic action of transducing phage / $c I. Mašlaňová, S. Stříbná, J. Doškař, R. Pantůček,

520    9_

$a The transduction mediated by bacteriophages is considered to be one of the primary driving forces in horizontal gene transfer in staphylococci, which is crucial to their adaptation and successful evolution. For a transduction to be effective, it is generally accepted that the recipient strain should be susceptible to the transducing phage. In this study, we demonstrate that the plasmid DNAs are effectively transduced into the recipient Staphylococcus aureus strains in spite of their insensitivity to the lytic action of the transducing phage, provided that these phages adsorb effectively to the bacterial cells. The tetracycline and penicillinase plasmids were transduced to insensitive laboratory and clinical strains by bacteriophages ϕ29, ϕ52A and ϕ80α as well as by prophage ϕ53 and naturally occurring prophages induced from donor lysogenic strains. Comparable frequencies of transduction were achieved in both phage-sensitive and phage-insensitive recipient strains. We have demonstrated that such mechanisms as the restriction of DNA and lysogenic immunity which are responsible for insensitivity of cells to phages may not be a barrier to the transfer, maintenance and effective spread of plasmids to a wider range of potential recipients in the staphylococcal population.

650    _2

$a antibakteriální látky $x farmakologie $7 D000900

650    _2

$a mnohočetná bakteriální léková rezistence $7 D024901

650    _2

$a lyzogenie $7 D008242

650    _2

$a penicilinasa $x genetika $7 D010405

650    12

$a plazmidy $7 D010957

650    _2

$a profágy $x genetika $x fyziologie $7 D039002

650    _2

$a stafylokokové infekce $x mikrobiologie $7 D013203

650    _2

$a stafylokokové bakteriofágy $x genetika $x fyziologie $7 D013204

650    _2

$a Staphylococcus aureus $x genetika $x virologie $7 D013211

650    _2

$a tetracyklin $x farmakologie $7 D013752

650    12

$a transdukce genetická $7 D014161

655    _2

$a časopisecké články $7 D016428

655    _2

$a práce podpořená grantem $7 D013485

700    1_

$a Stříbná, Sabina $u Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, CZ-611 37 Brno, Czech Republic.

700    1_

$a Doškař, Jiří $u Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, CZ-611 37 Brno, Czech Republic.

700    1_

$a Pantůček, Roman $u Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, CZ-611 37 Brno, Czech Republic.

773    0_

$w MED00001792 $t FEMS microbiology letters $x 1574-6968 $g Roč. 363, č. 19 (2016)

856    41

$u https://pubmed.ncbi.nlm.nih.gov/27609232 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20180515 $b ABA008

991    __

$a 20180521090808 $b ABA008

999    __

$a ok $b bmc $g 1300680 $s 1013896

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2016 $b 363 $c 19 $e 20160908 $i 1574-6968 $m FEMS microbiology letters $n FEMS Microbiol Lett $x MED00001792

LZP    __

$a Pubmed-20180515