Introduction: At present, there are no strong predictors, nor a useful scoring system, that clearly identifies patients at risk for anastomotic leakage. Aim: This study aimed to investigate a new method that assesses this risk by monitoring levels of neopterin, tryptophan, and kynurenine, in bodily fluids. Material and methods: This prospective study included patients who underwent elective rectal resection for carcinoma. The basic condition for inclusion was rectal anastomosis using the double-stapling technique. Preoperative levels of neopterin, tryptophan, kynurenine, and their ratios, were assessed with blood and urine samples. These levels were then monitored for 6 postoperative days in venous blood, urine, and abdominal drainage fluid. Results: A total of 42 patients were enrolled in the study. Thirty-six patients underwent a laparoscopic resection and 6 patients had an open procedure. No differences were found among neopterin, tryptophan, and kynurenine serum levels. However, the groups were observed to have significant differences in the urinary neopterin/creatinine ratio: the preoperative neopterin/creatinine ratio was 139.5 μmol/mol in the group with leakage, vs 114.8 μmol/mol in the group without complications, p = 0.037. The same results were observed during the postoperative period, p = 0.012. Additionally, the group with complications had a higher mean value of neopterin in drainage fluid, p = 0.048. Conclusions: Our study demonstrated that high preoperative levels of urinary neopterin could be interpreted as a risk for anastomotic leakage. Moreover, pathological levels of neopterin in urine and abdominal drainage fluid could be useful for early identification of anastomotic leakage during the postoperative period prior to its clinical development.

Department of Informatics and Quantitative Methods Faculty of Informatics and Management University of Hradec Kralove Czech Republic

Department of Surgery University Hospital Hradec Kralove Charles University Prague Faculty of Medicine in Hradec Kralove Czech Republic

Department of Surgery University Hospital Hradec Kralove Charles University Prague Faculty of Medicine in Hradec Kralove Czech Republic Department of Military Surgery Faculty of Military Health Sciences in Hradec Kralove University of Defence in Brno Czech Republic

Research Laboratory at 3 Internal Gerontometabolic Clinic University Hospital Hradec Kralove Czech Republic Department of Analytical Chemistry Faculty of Pharmacy in Hradec Kralove Charles University Prague Czech Republic

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