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Polyphasic data support the splitting of Aspergillus candidus into two species; proposal of Aspergillus dobrogensis sp. nov
V. Hubka, A. Nováková, Ž. Jurjević, F. Sklenář, JC. Frisvad, J. Houbraken, MC. Arendrup, KM. Jørgensen, JPZ. Siqueira, J. Gené, M. Kolařík,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články
NLK
Free Medical Journals
od 1951 do Před 2 roky
Freely Accessible Science Journals
od 2000 do Před 1 rokem
PubMed
29458472
DOI
10.1099/ijsem.0.002583
Knihovny.cz E-zdroje
- MeSH
- antifungální látky farmakologie MeSH
- Aspergillus klasifikace účinky léků MeSH
- Bayesova věta MeSH
- DNA fungální genetika MeSH
- fenotyp MeSH
- fylogeneze * MeSH
- mikrobiální testy citlivosti MeSH
- mykologické určovací techniky MeSH
- sekvenční analýza DNA MeSH
- Publikační typ
- časopisecké články MeSH
Aspergillus candidus is a species frequently isolated from stored grain, food, indoor environments, soil and occasionally also from clinical material. Recent bioprospecting studies highlighted the potential of using A. candidus and its relatives in various industrial sectors as a result of their significant production of enzymes and bioactive compounds. A high genetic variability was observed among A. candidus isolates originating from various European countries and the USA, that were mostly isolated from indoor environments, caves and clinical material. The A. candidus sensu lato isolates were characterized by DNA sequencing of four genetic loci, and agreement between molecular species delimitation results, morphological characters and exometabolite spectra were studied. Classical phylogenetic methods (maximum likelihood, Bayesian inference) and species delimitation methods based on the multispecies coalescent model supported recognition of up to three species in A. candidus sensu lato. After evaluation of phenotypic data, a broader species concept was adopted, and only one new species, Aspergillus dobrogensis, was proposed. This species is represented by 22 strains originating from seven countries (ex-type strain CCF 4651T=NRRL 62821T=IBT 32697T=CBS 143370T) and its differentiation from A. candidus is relevant for bioprospecting studies because these species have different exometabolite profiles. Evaluation of the antifungal susceptibility of section Candidi members to six antifungals using the reference EUCAST method showed that all species have low minimum inhibitory concentrations for all tested antifungals. These results suggest applicability of a wide spectrum of antifungal agents for treatment of infections caused by species from section Candidi.
Department of Biotechnology and Biomedicine Technical University of Denmark Kongens Lyngby Denmark
EMSL Analytical Inc Cinnaminson New Jersey USA
Unit of Mycology Statens Serum Institut Copenhagen Denmark
Westerdijk Fungal Biodiversity Institute Utrecht The Netherlands
Citace poskytuje Crossref.org
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- $a Hubka, Vít, $d 1986- $7 mzk2016904736 $u 2Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic. 1Department of Botany, Faculty of Science, Charles University, Prague, Czech Republic.
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- $a Aspergillus candidus is a species frequently isolated from stored grain, food, indoor environments, soil and occasionally also from clinical material. Recent bioprospecting studies highlighted the potential of using A. candidus and its relatives in various industrial sectors as a result of their significant production of enzymes and bioactive compounds. A high genetic variability was observed among A. candidus isolates originating from various European countries and the USA, that were mostly isolated from indoor environments, caves and clinical material. The A. candidus sensu lato isolates were characterized by DNA sequencing of four genetic loci, and agreement between molecular species delimitation results, morphological characters and exometabolite spectra were studied. Classical phylogenetic methods (maximum likelihood, Bayesian inference) and species delimitation methods based on the multispecies coalescent model supported recognition of up to three species in A. candidus sensu lato. After evaluation of phenotypic data, a broader species concept was adopted, and only one new species, Aspergillus dobrogensis, was proposed. This species is represented by 22 strains originating from seven countries (ex-type strain CCF 4651T=NRRL 62821T=IBT 32697T=CBS 143370T) and its differentiation from A. candidus is relevant for bioprospecting studies because these species have different exometabolite profiles. Evaluation of the antifungal susceptibility of section Candidi members to six antifungals using the reference EUCAST method showed that all species have low minimum inhibitory concentrations for all tested antifungals. These results suggest applicability of a wide spectrum of antifungal agents for treatment of infections caused by species from section Candidi.
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