-
Je něco špatně v tomto záznamu ?
Trehalose During Two Stress Responses in Acanthamoeba: Differentiation Between Encystation and Pseudocyst Formation
E. Bínová, D. Bína, DA. Ashford, J. Thomas-Oates, E. Nohýnková,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
- MeSH
- Acanthamoeba castellanii genetika metabolismus MeSH
- fylogeneze MeSH
- genom protozoální * MeSH
- messenger RNA genetika metabolismus MeSH
- metabolické sítě a dráhy MeSH
- protozoální proteiny genetika metabolismus MeSH
- RNA protozoální genetika metabolismus MeSH
- trehalosa biosyntéza MeSH
- Publikační typ
- časopisecké články MeSH
The non-reducing disaccharide trehalose can serve as a protectant against a range of environmental stressors, such as heat, cold, or dehydration, in both prokaryotes and eukaryotes, with the exception of vertebrates. Here, we analyzed trehalose metabolism in the facultatively parasitic organism Acanthamoeba castellanii, known to respond to unfavorable external conditions by forming two resistant stages: a cyst, produced in the case of chronic stress, and a pseudocyst, formed in reaction to acute stress. The possible role of trehalose in the resistant stages was investigated using a combination of bioinformatic, molecular biological and biochemical approaches. Genes for enzymes from a widespread trehalose-6-synthase-trehalose-6-phosphate phosphatase (TPS-TPP) pathway and a prokaryotic trehalose synthase (TreS) pathway were identified. The expression patterns of the genes during encystation and pseudocyst formation were analyzed and correlated with the time course of cellular trehalose content determined mass spectrometrically. The data clearly demonstrate fundamental differences between encystation and pseudocyst formation at the level of cellular metabolism.
Centre of Excellence in Mass Spectrometry University of York Heslington York YO10 5DD UK
Department of Chemistry University of York Heslington York YO10 5DD UK
Technology Facility Department of Biology University of York Heslington York YO10 5DD UK
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18024511
- 003
- CZ-PrNML
- 005
- 20180718104432.0
- 007
- ta
- 008
- 180709s2017 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.protis.2017.09.001 $2 doi
- 035 __
- $a (PubMed)29100111
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Bínová, Eva $u Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University and General Faculty Hospital, 128 00 Prague, Czech Republic.
- 245 10
- $a Trehalose During Two Stress Responses in Acanthamoeba: Differentiation Between Encystation and Pseudocyst Formation / $c E. Bínová, D. Bína, DA. Ashford, J. Thomas-Oates, E. Nohýnková,
- 520 9_
- $a The non-reducing disaccharide trehalose can serve as a protectant against a range of environmental stressors, such as heat, cold, or dehydration, in both prokaryotes and eukaryotes, with the exception of vertebrates. Here, we analyzed trehalose metabolism in the facultatively parasitic organism Acanthamoeba castellanii, known to respond to unfavorable external conditions by forming two resistant stages: a cyst, produced in the case of chronic stress, and a pseudocyst, formed in reaction to acute stress. The possible role of trehalose in the resistant stages was investigated using a combination of bioinformatic, molecular biological and biochemical approaches. Genes for enzymes from a widespread trehalose-6-synthase-trehalose-6-phosphate phosphatase (TPS-TPP) pathway and a prokaryotic trehalose synthase (TreS) pathway were identified. The expression patterns of the genes during encystation and pseudocyst formation were analyzed and correlated with the time course of cellular trehalose content determined mass spectrometrically. The data clearly demonstrate fundamental differences between encystation and pseudocyst formation at the level of cellular metabolism.
- 650 _2
- $a Acanthamoeba castellanii $x genetika $x metabolismus $7 D048248
- 650 12
- $a genom protozoální $7 D018503
- 650 _2
- $a metabolické sítě a dráhy $7 D053858
- 650 _2
- $a fylogeneze $7 D010802
- 650 _2
- $a protozoální proteiny $x genetika $x metabolismus $7 D015800
- 650 _2
- $a messenger RNA $x genetika $x metabolismus $7 D012333
- 650 _2
- $a RNA protozoální $x genetika $x metabolismus $7 D016053
- 650 _2
- $a trehalosa $x biosyntéza $7 D014199
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Bína, David $u Biology Centre of the Czech Academy of Sciences and Faculty of Science, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic.
- 700 1_
- $a Ashford, David A $u Centre of Excellence in Mass Spectrometry, University of York, Heslington, York, YO10 5DD, UK; Technology Facility (Proteomics & Analytical Biochemistry Laboratory), Department of Biology, University of York, Heslington, York, YO10 5DD, UK.
- 700 1_
- $a Thomas-Oates, Jane $u Centre of Excellence in Mass Spectrometry, University of York, Heslington, York, YO10 5DD, UK; Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK.
- 700 1_
- $a Nohýnková, Eva $u Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University and General Faculty Hospital, 128 00 Prague, Czech Republic. Electronic address: enohy@lf1.cuni.cz.
- 773 0_
- $w MED00005138 $t Protist $x 1618-0941 $g Roč. 168, č. 6 (2017), s. 649-662
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/29100111 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20180709 $b ABA008
- 991 __
- $a 20180718104733 $b ABA008
- 999 __
- $a ok $b bmc $g 1316642 $s 1021432
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 168 $c 6 $d 649-662 $e 20170914 $i 1618-0941 $m Protist $n Protist (Jena, Print) $x MED00005138
- LZP __
- $a Pubmed-20180709
