-
Je něco špatně v tomto záznamu ?
BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana
S. Kitakura, M. Adamowski, Y. Matsuura, L. Santuari, H. Kouno, K. Arima, CS. Hardtke, J. Friml, T. Kakimoto, H. Tanaka,
Jazyk angličtina Země Japonsko
Typ dokumentu časopisecké články
NLK
Free Medical Journals
od 1996 do Před 1 rokem
Open Access Digital Library
od 1996-01-01
Medline Complete (EBSCOhost)
od 1996-01-01 do Před 1 rokem
PubMed
29016942
DOI
10.1093/pcp/pcx118
Knihovny.cz E-zdroje
- MeSH
- ADP-ribosylační faktory genetika metabolismus MeSH
- alely MeSH
- Arabidopsis účinky léků metabolismus MeSH
- brefeldin A farmakologie MeSH
- buněčná membrána účinky léků metabolismus MeSH
- endozomy účinky léků metabolismus MeSH
- fenotyp MeSH
- klonování DNA MeSH
- kompartmentace buňky MeSH
- nesmyslný kodon genetika MeSH
- proteiny huseníčku genetika metabolismus MeSH
- semenáček účinky léků růst a vývoj MeSH
- trans-Golgiho síť účinky léků metabolismus MeSH
- transport proteinů účinky léků MeSH
- výměnné faktory guaninnukleotidů metabolismus MeSH
- zelené fluorescenční proteiny metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
Membrane traffic at the trans-Golgi network (TGN) is crucial for correctly distributing various membrane proteins to their destination. Polarly localized auxin efflux proteins, including PIN-FORMED1 (PIN1), are dynamically transported between the endosomes and the plasma membrane (PM) in the plant cells. The intracellular trafficking of PIN1 protein is sensitive to the fungal toxin brefeldin A (BFA), which is known to inhibit guanine nucleotide exchange factors for ADP ribosylation factors (ARF GEFs) such as GNOM. However, the molecular details of the BFA-sensitive trafficking pathway have not been fully revealed. In a previous study, we identified an Arabidopsis mutant BFA-visualized endocytic trafficking defective 3 (ben3) which exhibited reduced sensitivity to BFA in terms of BFA-induced intracellular PIN1 agglomeration. Here, we show that BEN3 encodes a member of BIG family ARF GEFs, BIG2. BEN3/BIG2 tagged with fluorescent proteins co-localized with markers for the TGN/early endosome (EE). Inspection of conditionally induced de novo synthesized PIN1 confirmed that its secretion to the PM is BFA sensitive, and established BEN3/BIG2 as a crucial component of this BFA action at the level of the TGN/EE. Furthermore, ben3 mutation alleviated BFA-induced agglomeration of another TGN-localized ARF GEF, BEN1/MIN7. Taken together, our results suggest that BEN3/BIG2 is an ARF GEF component, which confers BFA sensitivity to the TGN/EE in Arabidopsis.
Department of Biological Science Graduate School of Science Osaka University Osaka 560 0043 Japan
Institute of Science and Technology Austria 3400 Klosterneuburg Austria
Institute of Science and Technology Austria Kamenice 5 Brno CZ 625 00 Czech Republic
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18024583
- 003
- CZ-PrNML
- 005
- 20180719090545.0
- 007
- ta
- 008
- 180709s2017 ja f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1093/pcp/pcx118 $2 doi
- 035 __
- $a (PubMed)29016942
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ja
- 100 1_
- $a Kitakura, Saeko $u Department of Biological Science, Graduate School of Science, Osaka University, Osaka, 560-0043 Japan.
- 245 10
- $a BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana / $c S. Kitakura, M. Adamowski, Y. Matsuura, L. Santuari, H. Kouno, K. Arima, CS. Hardtke, J. Friml, T. Kakimoto, H. Tanaka,
- 520 9_
- $a Membrane traffic at the trans-Golgi network (TGN) is crucial for correctly distributing various membrane proteins to their destination. Polarly localized auxin efflux proteins, including PIN-FORMED1 (PIN1), are dynamically transported between the endosomes and the plasma membrane (PM) in the plant cells. The intracellular trafficking of PIN1 protein is sensitive to the fungal toxin brefeldin A (BFA), which is known to inhibit guanine nucleotide exchange factors for ADP ribosylation factors (ARF GEFs) such as GNOM. However, the molecular details of the BFA-sensitive trafficking pathway have not been fully revealed. In a previous study, we identified an Arabidopsis mutant BFA-visualized endocytic trafficking defective 3 (ben3) which exhibited reduced sensitivity to BFA in terms of BFA-induced intracellular PIN1 agglomeration. Here, we show that BEN3 encodes a member of BIG family ARF GEFs, BIG2. BEN3/BIG2 tagged with fluorescent proteins co-localized with markers for the TGN/early endosome (EE). Inspection of conditionally induced de novo synthesized PIN1 confirmed that its secretion to the PM is BFA sensitive, and established BEN3/BIG2 as a crucial component of this BFA action at the level of the TGN/EE. Furthermore, ben3 mutation alleviated BFA-induced agglomeration of another TGN-localized ARF GEF, BEN1/MIN7. Taken together, our results suggest that BEN3/BIG2 is an ARF GEF component, which confers BFA sensitivity to the TGN/EE in Arabidopsis.
- 650 _2
- $a ADP-ribosylační faktory $x genetika $x metabolismus $7 D020727
- 650 _2
- $a alely $7 D000483
- 650 _2
- $a Arabidopsis $x účinky léků $x metabolismus $7 D017360
- 650 _2
- $a proteiny huseníčku $x genetika $x metabolismus $7 D029681
- 650 _2
- $a brefeldin A $x farmakologie $7 D020126
- 650 _2
- $a kompartmentace buňky $7 D002451
- 650 _2
- $a buněčná membrána $x účinky léků $x metabolismus $7 D002462
- 650 _2
- $a klonování DNA $7 D003001
- 650 _2
- $a nesmyslný kodon $x genetika $7 D018389
- 650 _2
- $a endozomy $x účinky léků $x metabolismus $7 D011992
- 650 _2
- $a zelené fluorescenční proteiny $x metabolismus $7 D049452
- 650 _2
- $a výměnné faktory guaninnukleotidů $x metabolismus $7 D020662
- 650 _2
- $a fenotyp $7 D010641
- 650 _2
- $a transport proteinů $x účinky léků $7 D021381
- 650 _2
- $a semenáček $x účinky léků $x růst a vývoj $7 D036226
- 650 _2
- $a trans-Golgiho síť $x účinky léků $x metabolismus $7 D021601
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Adamowski, Maciek $u Institute of Science and Technology Austria (IST Austria), 3400 Klosterneuburg, Austria.
- 700 1_
- $a Matsuura, Yuki $u Department of Biological Science, Graduate School of Science, Osaka University, Osaka, 560-0043 Japan. Interdisciplinary Program for Biomedical Sciences (IPBS), Institute for Academic Initiatives, Osaka University, Suita, Japan.
- 700 1_
- $a Santuari, Luca $u Plant Developmental Biology, Wageningen University and Research Centre, 6708PB Wageningen, The Netherlands.
- 700 1_
- $a Kouno, Hirotaka $u Department of Biological Science, Graduate School of Science, Osaka University, Osaka, 560-0043 Japan.
- 700 1_
- $a Arima, Kohei $u Department of Biological Science, Graduate School of Science, Osaka University, Osaka, 560-0043 Japan.
- 700 1_
- $a Hardtke, Christian S $u Plant Developmental Biology, Wageningen University and Research Centre, 6708PB Wageningen, The Netherlands.
- 700 1_
- $a Friml, Jirí $u Institute of Science and Technology Austria (IST Austria), 3400 Klosterneuburg, Austria. Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology (CEITEC), Masaryk University (MU), Kamenice 5, Brno, CZ-625 00 Czech Republic.
- 700 1_
- $a Kakimoto, Tatsuo $u Department of Biological Science, Graduate School of Science, Osaka University, Osaka, 560-0043 Japan.
- 700 1_
- $a Tanaka, Hirokazu $u Department of Biological Science, Graduate School of Science, Osaka University, Osaka, 560-0043 Japan.
- 773 0_
- $w MED00003836 $t Plant & cell physiology $x 1471-9053 $g Roč. 58, č. 10 (2017), s. 1801-1811
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/29016942 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20180709 $b ABA008
- 991 __
- $a 20180719090846 $b ABA008
- 999 __
- $a ok $b bmc $g 1316714 $s 1021504
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 58 $c 10 $d 1801-1811 $i 1471-9053 $m Plant and Cell Physiology $n Plant Cell Physiol $x MED00003836
- LZP __
- $a Pubmed-20180709