Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis

M. Kralikova, I. Crha, M. Huser, J. Melounova, J. Zakova, M. Matejovicova, P. Ventruba,

. 2017 ; 49 (7) : . [pub] 20160913

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18025310

The aims of this study were to determine concentrations of total homocysteine, cysteine, cysteinylglycine and glutathione in spermatozoa, seminal fluid and blood plasma and to analyse their relationships with sperm parameters. For this reason, a new highly effective method of spermatozoa lysis was developed, using methanol, freezing and subsequent thawing in ultrasonic bath. An HPLC-FD assay was conducted on thiols concentrations in lysed spermatozoa, seminal fluid and blood plasma. Concentrations of thiols in spermatozoa were significantly lower in men with normozoospermia than in samples with pathological semen parameters. Statistical analysis found significant correlations between thiol concentrations in spermatozoa and semen parameters, while the same analysis with thiol concentrations in seminal fluid was substantially less powerful. Only cysteinylglycine concentrations in seminal fluid significantly correlated with pathological semen parameters. No significant differences or correlations were found with blood plasma concentrations.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18025310
003      
CZ-PrNML
005      
20180713094624.0
007      
ta
008      
180709s2017 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1111/and.12702 $2 doi
035    __
$a (PubMed)27620273
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Kralikova, M $u Department of Biochemistry, Masaryk University Medical School, Brno, Czech Republic.
245    14
$a The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis / $c M. Kralikova, I. Crha, M. Huser, J. Melounova, J. Zakova, M. Matejovicova, P. Ventruba,
520    9_
$a The aims of this study were to determine concentrations of total homocysteine, cysteine, cysteinylglycine and glutathione in spermatozoa, seminal fluid and blood plasma and to analyse their relationships with sperm parameters. For this reason, a new highly effective method of spermatozoa lysis was developed, using methanol, freezing and subsequent thawing in ultrasonic bath. An HPLC-FD assay was conducted on thiols concentrations in lysed spermatozoa, seminal fluid and blood plasma. Concentrations of thiols in spermatozoa were significantly lower in men with normozoospermia than in samples with pathological semen parameters. Statistical analysis found significant correlations between thiol concentrations in spermatozoa and semen parameters, while the same analysis with thiol concentrations in seminal fluid was substantially less powerful. Only cysteinylglycine concentrations in seminal fluid significantly correlated with pathological semen parameters. No significant differences or correlations were found with blood plasma concentrations.
650    _2
$a mladiství $7 D000293
650    _2
$a dospělí $7 D000328
650    _2
$a frakcionace buněk $x metody $7 D002458
650    _2
$a homocystein $x analýza $x krev $7 D006710
650    _2
$a lidé $7 D006801
650    _2
$a mužská infertilita $x krev $x metabolismus $7 D007248
650    _2
$a intracelulární prostor $x chemie $7 D042541
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a lidé středního věku $7 D008875
650    _2
$a sperma $x chemie $7 D012661
650    _2
$a spermie $x chemie $x ultrastruktura $7 D013094
650    _2
$a sulfhydrylové sloučeniny $x analýza $x krev $7 D013438
655    _2
$a časopisecké články $7 D016428
700    1_
$a Crha, I $u Department of Gynecology and Obstetrics, Brno University Hospital and Masaryk University Medical School, Brno, Czech Republic.
700    1_
$a Huser, M $u Department of Gynecology and Obstetrics, Brno University Hospital and Masaryk University Medical School, Brno, Czech Republic.
700    1_
$a Melounova, J $u Department of Biochemistry, Masaryk University Medical School, Brno, Czech Republic.
700    1_
$a Zakova, J $u Department of Gynecology and Obstetrics, Brno University Hospital and Masaryk University Medical School, Brno, Czech Republic.
700    1_
$a Matejovicova, M $u Department of Biochemistry, Masaryk University Medical School, Brno, Czech Republic.
700    1_
$a Ventruba, P $u Department of Gynecology and Obstetrics, Brno University Hospital and Masaryk University Medical School, Brno, Czech Republic.
773    0_
$w MED00000345 $t Andrologia $x 1439-0272 $g Roč. 49, č. 7 (2017)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/27620273 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20180709 $b ABA008
991    __
$a 20180713094917 $b ABA008
999    __
$a ok $b bmc $g 1317441 $s 1022231
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 49 $c 7 $e 20160913 $i 1439-0272 $m Andrologia $n Andrologia $x MED00000345
LZP    __
$a Pubmed-20180709

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace