Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Virion structure and genome delivery mechanism of sacbrood honeybee virus

M. Procházková, T. Füzik, K. Škubník, J. Moravcová, Z. Ubiparip, A. Přidal, P. Plevka,

. 2018 ; 115 (30) : 7759-7764. [pub] 20180709

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc18033011
E-zdroje Online Plný text

NLK Free Medical Journals od 1915 do Před 6 měsíci
Freely Accessible Science Journals od 1915 do Před 6 měsíci
PubMed Central od 1915 do Před 6 měsíci
Europe PubMed Central od 1915 do Před 6 měsíci
Open Access Digital Library od 1915-01-15
Open Access Digital Library od 1915-01-01

Infection by sacbrood virus (SBV) from the family Iflaviridae is lethal to honey bee larvae but only rarely causes the collapse of honey bee colonies. Despite the negative effect of SBV on honey bees, the structure of its particles and mechanism of its genome delivery are unknown. Here we present the crystal structure of SBV virion and show that it contains 60 copies of a minor capsid protein (MiCP) attached to the virion surface. No similar MiCPs have been previously reported in any of the related viruses from the order Picornavirales. The location of the MiCP coding sequence within the SBV genome indicates that the MiCP evolved from a C-terminal extension of a major capsid protein by the introduction of a cleavage site for a virus protease. The exposure of SBV to acidic pH, which the virus likely encounters during cell entry, induces the formation of pores at threefold and fivefold axes of the capsid that are 7 Å and 12 Å in diameter, respectively. This is in contrast to vertebrate picornaviruses, in which the pores along twofold icosahedral symmetry axes are currently considered the most likely sites for genome release. SBV virions lack VP4 subunits that facilitate the genome delivery of many related dicistroviruses and picornaviruses. MiCP subunits induce liposome disruption in vitro, indicating that they are functional analogs of VP4 subunits and enable the virus genome to escape across the endosome membrane into the cell cytoplasm.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18033011
003      
CZ-PrNML
005      
20181010125502.0
007      
ta
008      
181008s2018 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1073/pnas.1722018115 $2 doi
035    __
$a (PubMed)29987012
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Procházková, Michaela $u Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech Republic.
245    10
$a Virion structure and genome delivery mechanism of sacbrood honeybee virus / $c M. Procházková, T. Füzik, K. Škubník, J. Moravcová, Z. Ubiparip, A. Přidal, P. Plevka,
520    9_
$a Infection by sacbrood virus (SBV) from the family Iflaviridae is lethal to honey bee larvae but only rarely causes the collapse of honey bee colonies. Despite the negative effect of SBV on honey bees, the structure of its particles and mechanism of its genome delivery are unknown. Here we present the crystal structure of SBV virion and show that it contains 60 copies of a minor capsid protein (MiCP) attached to the virion surface. No similar MiCPs have been previously reported in any of the related viruses from the order Picornavirales. The location of the MiCP coding sequence within the SBV genome indicates that the MiCP evolved from a C-terminal extension of a major capsid protein by the introduction of a cleavage site for a virus protease. The exposure of SBV to acidic pH, which the virus likely encounters during cell entry, induces the formation of pores at threefold and fivefold axes of the capsid that are 7 Å and 12 Å in diameter, respectively. This is in contrast to vertebrate picornaviruses, in which the pores along twofold icosahedral symmetry axes are currently considered the most likely sites for genome release. SBV virions lack VP4 subunits that facilitate the genome delivery of many related dicistroviruses and picornaviruses. MiCP subunits induce liposome disruption in vitro, indicating that they are functional analogs of VP4 subunits and enable the virus genome to escape across the endosome membrane into the cell cytoplasm.
650    _2
$a zvířata $7 D000818
650    _2
$a včely $x virologie $7 D001516
650    12
$a virové plášťové proteiny $x chemie $x metabolismus $7 D036022
650    _2
$a krystalografie rentgenová $7 D018360
650    _2
$a endozomy $x chemie $x metabolismus $x virologie $7 D011992
650    12
$a genom virový $7 D016679
650    12
$a RNA-viry $x chemie $x metabolismus $7 D012328
650    12
$a virion $x chemie $x metabolismus $7 D014771
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Füzik, Tibor $u Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech Republic.
700    1_
$a Škubník, Karel $u Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech Republic.
700    1_
$a Moravcová, Jana $u Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech Republic.
700    1_
$a Ubiparip, Zorica $u Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech Republic.
700    1_
$a Přidal, Antonín $u Faculty of Agronomy, Mendel University, 613 00 Brno, Czech Republic.
700    1_
$a Plevka, Pavel $u Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech Republic; pavel.plevka@ceitec.muni.cz.
773    0_
$w MED00010472 $t Proceedings of the National Academy of Sciences of the United States of America $x 1091-6490 $g Roč. 115, č. 30 (2018), s. 7759-7764
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29987012 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20181008 $b ABA008
991    __
$a 20181010125951 $b ABA008
999    __
$a ok $b bmc $g 1340771 $s 1030005
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 115 $c 30 $d 7759-7764 $e 20180709 $i 1091-6490 $m Proceedings of the National Academy of Sciences of the United States of America $n Proc Natl Acad Sci U S A $x MED00010472
LZP    __
$a Pubmed-20181008

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...