During aetiological study of diseased red clover (Trifolium pratense L.) using high throughput sequencing, a novel virus with a 10 kb genome divided into two segments was discovered. The virus, tentatively named red clover associated varicosavirus (RCaVV), is phylogenetically related to classifiable members of the genus Varicosavirus (family Rhabdoviridae, order Mononegavirales). Analysis of mRNA levels from the individual RCaVV genes suggested possible differences in transcription regulation between rhabdoviruses with divided and undivided genomes.

Department of Plant Virology Institute of Plant Molecular Biology Biology Centre of the Academy of Sciences of the Czech Republic v v i Branišovská 31 370 05 České Budějovice Czech Republic

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$a Koloniuk, Igor $u Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05, České Budějovice, Czech Republic. koloniuk@umbr.cas.cz.

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$a Identification and molecular characterization of a novel varicosa-like virus from red clover / $c I. Koloniuk, J. Fránová, T. Sarkisova, J. Přibylová, O. Lenz, K. Petrzik, J. Špak,

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$a During aetiological study of diseased red clover (Trifolium pratense L.) using high throughput sequencing, a novel virus with a 10 kb genome divided into two segments was discovered. The virus, tentatively named red clover associated varicosavirus (RCaVV), is phylogenetically related to classifiable members of the genus Varicosavirus (family Rhabdoviridae, order Mononegavirales). Analysis of mRNA levels from the individual RCaVV genes suggested possible differences in transcription regulation between rhabdoviruses with divided and undivided genomes.

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$a Fránová, Jana $u Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05, České Budějovice, Czech Republic.

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$a Sarkisova, Tatiana $u Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05, České Budějovice, Czech Republic.

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$a Přibylová, Jaroslava $u Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05, České Budějovice, Czech Republic.

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$a Lenz, Ondřej $u Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05, České Budějovice, Czech Republic.

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$a Petrzik, Karel $u Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05, České Budějovice, Czech Republic.

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$a Špak, Josef $u Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05, České Budějovice, Czech Republic.

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