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A simple approach for fabrication of optical affinity-based bioanalytical microsystem on polymeric PEN foils

A. Semerádtová, M. Štofik, O. Neděla, O. Staněk, P. Slepička, Z. Kolská, J. Malý,

. 2018 ; 165 (-) : 28-36. [pub] 20180202

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18033315

Herein, we report a novel concept of low-cost flexible platform for fluorescence-based biosensor. The surface of polyethylene naphthalate (PEN) foil was exposed to KrF excimer laser through a photolitographic contact mask. Laser initiated surface modification resulted in micro-patterned areas with surface functional groups available for localized covalent immobilization of biotin. High affinity binding protein (albumin-binding domain (ABD) of protein G, Streptococcus G148) recognizing human serum albumin (HSA), genetically fused with streptavidin (SA-ABDwt), was immobilized on the micro-patterned surface through biotin-streptavidin coupling. Fluorescently labelled HSA analyte was detected in several blocking environments, in 1% bovine serum albumin (BSA) and 6% fetal serum albumin (FBS), respectively. We conclude that the presented novel concept enabled us to micropattern functional biosensing layers on the surface of PEN foil in a fast and easy way. It brings all necessary aspects for continuous roll-to-roll fabrication of low-cost optical bioanalytical devices.

Citace poskytuje Crossref.org

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$a Štofik, Marcel $u Department of Biology and Material Centre, Faculty of Science, University of J.E. Purkinje, 400 96 Ústí nad Labem, Czech Republic. Electronic address: marcel.stofik@ujep.cz.
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$a Slepička, Petr $u Department of Solid State Engineering, University of Chemistry and Technology, 166 28 Prague, Czech Republic.
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