-
Je něco špatně v tomto záznamu ?
Molecular Farming in Barley: Development of a Novel Production Platform to Produce Human Antimicrobial Peptide LL-37
E. Holásková, P. Galuszka, A. Mičúchová, M. Šebela, MT. Öz, I. Frébort,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
Odkazy
PubMed
29369519
DOI
10.1002/biot.201700628
Knihovny.cz E-zdroje
- MeSH
- geneticky modifikované rostliny genetika metabolismus MeSH
- ječmen (rod) genetika metabolismus MeSH
- kathelicidiny chemie genetika izolace a purifikace metabolismus MeSH
- lidé MeSH
- molekulární farmaření metody MeSH
- promotorové oblasti (genetika) genetika MeSH
- rekombinantní fúzní proteiny chemie genetika izolace a purifikace metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The peptide LL-37, a component of the human innate immune system, represents a promising drug candidate. In particular, the development of low-cost production platform technology is a critical bottleneck in its use in medicine. In the present study, a viable approach for the LL-37 production in transgenic barley is developed. First, comparative analyses of the effects of different fused peptide epitope tags applicable for accumulation and purification on LL-37 production yield are performed using transient expression in tobacco leaves. Following the selection of the most yielding fusion peptide strategies, eight different constructs for the expression of codon optimized chimeric LL-37 genes in transgenic barley plants are created. The expression of individual constructs is driven either by an endosperm-specific promoter of the barley B1 hordein gene or by the maize ubiquitin promoter. The transgenes are stably integrated into the barley genome and inherited in the subsequent generation. All transgenic lines show normal phenotypes and are fertile. LL-37 accumulated in the barley seeds up to 0.55 mg per 1 kg of grain. The fused epitope tags are cleaved off by the use of enterokinase. Furthermore, in planta produced LL-37 including the fused versions is biologically active.
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18033369
- 003
- CZ-PrNML
- 005
- 20181022130218.0
- 007
- ta
- 008
- 181008s2018 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1002/biot.201700628 $2 doi
- 035 __
- $a (PubMed)29369519
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Holásková, Edita $u Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University, Olomouc, 783 71, Czech Republic.
- 245 10
- $a Molecular Farming in Barley: Development of a Novel Production Platform to Produce Human Antimicrobial Peptide LL-37 / $c E. Holásková, P. Galuszka, A. Mičúchová, M. Šebela, MT. Öz, I. Frébort,
- 520 9_
- $a The peptide LL-37, a component of the human innate immune system, represents a promising drug candidate. In particular, the development of low-cost production platform technology is a critical bottleneck in its use in medicine. In the present study, a viable approach for the LL-37 production in transgenic barley is developed. First, comparative analyses of the effects of different fused peptide epitope tags applicable for accumulation and purification on LL-37 production yield are performed using transient expression in tobacco leaves. Following the selection of the most yielding fusion peptide strategies, eight different constructs for the expression of codon optimized chimeric LL-37 genes in transgenic barley plants are created. The expression of individual constructs is driven either by an endosperm-specific promoter of the barley B1 hordein gene or by the maize ubiquitin promoter. The transgenes are stably integrated into the barley genome and inherited in the subsequent generation. All transgenic lines show normal phenotypes and are fertile. LL-37 accumulated in the barley seeds up to 0.55 mg per 1 kg of grain. The fused epitope tags are cleaved off by the use of enterokinase. Furthermore, in planta produced LL-37 including the fused versions is biologically active.
- 650 _2
- $a kathelicidiny $x chemie $x genetika $x izolace a purifikace $x metabolismus $7 D054804
- 650 _2
- $a ječmen (rod) $x genetika $x metabolismus $7 D001467
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a molekulární farmaření $x metody $7 D058634
- 650 _2
- $a geneticky modifikované rostliny $x genetika $x metabolismus $7 D030821
- 650 _2
- $a promotorové oblasti (genetika) $x genetika $7 D011401
- 650 _2
- $a rekombinantní fúzní proteiny $x chemie $x genetika $x izolace a purifikace $x metabolismus $7 D011993
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Galuszka, Petr $u Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University, Olomouc, 783 71, Czech Republic.
- 700 1_
- $a Mičúchová, Alžbeta $u Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University, Olomouc, 783 71, Czech Republic.
- 700 1_
- $a Šebela, Marek $u Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University, Olomouc, 783 71, Czech Republic.
- 700 1_
- $a Öz, Mehmet Tufan $u Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University, Olomouc, 783 71, Czech Republic.
- 700 1_
- $a Frébort, Ivo $u Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University, Olomouc, 783 71, Czech Republic.
- 773 0_
- $w MED00182167 $t Biotechnology journal $x 1860-7314 $g Roč. 13, č. 6 (2018), s. e1700628
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/29369519 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20181008 $b ABA008
- 991 __
- $a 20181022130724 $b ABA008
- 999 __
- $a ok $b bmc $g 1340121 $s 1030363
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2018 $b 13 $c 6 $d e1700628 $e 20180208 $i 1860-7314 $m Biotechnology journal $n Biotechnol J $x MED00182167
- LZP __
- $a Pubmed-20181008