-
Je něco špatně v tomto záznamu ?
Multiple functions of CREB-binding protein during postembryonic development: identification of target genes
A. Roy, S. George, SR. Palli,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.
NLK
BioMedCentral
od 2000-12-01
BioMedCentral Open Access
od 2000
Directory of Open Access Journals
od 2000
Free Medical Journals
od 2000
PubMed Central
od 2000
Europe PubMed Central
od 2000 do 2020
ProQuest Central
od 2009-01-01
Open Access Digital Library
od 2000-07-01
Open Access Digital Library
od 2000-01-01
Open Access Digital Library
od 2000-01-01
Medline Complete (EBSCOhost)
od 2000-01-01
Health & Medicine (ProQuest)
od 2009-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2000
Springer Nature OA/Free Journals
od 2000-12-01
- MeSH
- exprese genu MeSH
- hmyzí proteiny antagonisté a inhibitory genetika metabolismus fyziologie MeSH
- juvenilní hormony farmakologie MeSH
- larva genetika metabolismus MeSH
- protein vázající CREB antagonisté a inhibitory genetika metabolismus fyziologie MeSH
- RNA interference MeSH
- Tribolium genetika růst a vývoj metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Research Support, N.I.H., Extramural MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
BACKGROUND: Juvenile hormones (JH) and ecdysteroids control postembryonic development in insects. They serve as valuable targets for pest management. Hence, understanding the molecular mechanisms of their action is of crucial importance. CREB-binding protein (CBP) is a universal transcriptional co-regulator. It controls the expression of several genes including those from hormone signaling pathways through co-activation of many transcription factors. However, the role of CBP during postembryonic development in insects is not well understood. Therefore, we have studied the role of CBP in postembryonic development in Tribolium, a model coleopteran insect. RESULTS: CBP is ubiquitously expressed in the red flour beetle, Tribolium castaneum. RNA interference (RNAi) mediated knockdown of CBP resulted in a decrease in JH induction of Kr-h1 gene expression in Tribolium larvae and led to a block in their development. Moreover, the injection of CBP double-stranded RNA (dsRNA) showed lethal phenotypes within 8 days of injection. RNA-seq and subsequent differential gene expression analysis identified CBP target genes in Tribolium. Knockdown of CBP caused a decrease in the expression of 1306 genes coding for transcription factors and other proteins associated with growth and development. Depletion of CBP impaired the expression of several JH response genes (e.g., Kr-h1, Hairy, early trypsin) and ecdysone response genes (EcR, E74, E75, and broad complex). Further, GO enrichment analyses of the downregulated genes showed enrichment in different functions including developmental processes, pigmentation, anatomical structure development, regulation of biological and cellular processes, etc. CONCLUSION: These data suggest diverse but crucial roles for CBP during postembryonic development in the coleopteran model insect, Tribolium. It can serve as a target for RNAi mediated pest management of this stored product pest.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18033425
- 003
- CZ-PrNML
- 005
- 20181009110144.0
- 007
- ta
- 008
- 181008s2017 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1186/s12864-017-4373-3 $2 doi
- 035 __
- $a (PubMed)29284404
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Roy, Amit $u Department of Entomology, College of Agriculture, University of Kentucky, Lexington, KY, 40546, USA. Present address, Faculty of Forestry and Wood Sciences, EXTEMIT-K, Czech University of Life Sciences, Kamýcká 1176, Prague 6, 165 21, Suchdol, Czech Republic.
- 245 10
- $a Multiple functions of CREB-binding protein during postembryonic development: identification of target genes / $c A. Roy, S. George, SR. Palli,
- 520 9_
- $a BACKGROUND: Juvenile hormones (JH) and ecdysteroids control postembryonic development in insects. They serve as valuable targets for pest management. Hence, understanding the molecular mechanisms of their action is of crucial importance. CREB-binding protein (CBP) is a universal transcriptional co-regulator. It controls the expression of several genes including those from hormone signaling pathways through co-activation of many transcription factors. However, the role of CBP during postembryonic development in insects is not well understood. Therefore, we have studied the role of CBP in postembryonic development in Tribolium, a model coleopteran insect. RESULTS: CBP is ubiquitously expressed in the red flour beetle, Tribolium castaneum. RNA interference (RNAi) mediated knockdown of CBP resulted in a decrease in JH induction of Kr-h1 gene expression in Tribolium larvae and led to a block in their development. Moreover, the injection of CBP double-stranded RNA (dsRNA) showed lethal phenotypes within 8 days of injection. RNA-seq and subsequent differential gene expression analysis identified CBP target genes in Tribolium. Knockdown of CBP caused a decrease in the expression of 1306 genes coding for transcription factors and other proteins associated with growth and development. Depletion of CBP impaired the expression of several JH response genes (e.g., Kr-h1, Hairy, early trypsin) and ecdysone response genes (EcR, E74, E75, and broad complex). Further, GO enrichment analyses of the downregulated genes showed enrichment in different functions including developmental processes, pigmentation, anatomical structure development, regulation of biological and cellular processes, etc. CONCLUSION: These data suggest diverse but crucial roles for CBP during postembryonic development in the coleopteran model insect, Tribolium. It can serve as a target for RNAi mediated pest management of this stored product pest.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a protein vázající CREB $x antagonisté a inhibitory $x genetika $x metabolismus $x fyziologie $7 D050882
- 650 _2
- $a exprese genu $7 D015870
- 650 _2
- $a hmyzí proteiny $x antagonisté a inhibitory $x genetika $x metabolismus $x fyziologie $7 D019476
- 650 _2
- $a juvenilní hormony $x farmakologie $7 D007605
- 650 _2
- $a larva $x genetika $x metabolismus $7 D007814
- 650 _2
- $a RNA interference $7 D034622
- 650 _2
- $a Tribolium $x genetika $x růst a vývoj $x metabolismus $7 D014231
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a Research Support, N.I.H., Extramural $7 D052061
- 655 _2
- $a Research Support, U.S. Gov't, Non-P.H.S. $7 D013486
- 700 1_
- $a George, Smitha $u Department of Entomology, College of Agriculture, University of Kentucky, Lexington, KY, 40546, USA.
- 700 1_
- $a Palli, Subba Reddy $u Department of Entomology, College of Agriculture, University of Kentucky, Lexington, KY, 40546, USA. rpalli@uky.edu.
- 773 0_
- $w MED00008181 $t BMC genomics $x 1471-2164 $g Roč. 18, č. 1 (2017), s. 996
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/29284404 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20181008 $b ABA008
- 991 __
- $a 20181009110631 $b ABA008
- 999 __
- $a ok $b bmc $g 1340146 $s 1030419
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 18 $c 1 $d 996 $e 20171229 $i 1471-2164 $m BMC genomics $n BMC Genomics $x MED00008181
- LZP __
- $a Pubmed-20181008