High levels of reactive oxygen species (ROS), which may be associated with reduced sperm quality, can be detected during cryopreservation of sperm of some species. Our objective was to investigate whether the addition of antioxidants to cryopreservation extenders influenced post-thaw sperm characteristics and fertility in Acipenser dabryanus, Acipenser sinensis and Acipenser baerii. Prior to freezing, sperm samples were diluted with a base extender as control or in extender supplemented with catalase (CAT), glutathione (GSH), cysteine (NAC), ascorbic acid (VC) or their paired combinations. Protective concentrations of CAT, GSH and VC in the three species were 25 U/ml, 0.25-0.5 mg/ml and 0.5 mg/ml, respectively. Cysteine showed no protective effect against ROS. The addition of CAT, GSH and VC positively affected either acrosome or membrane integrity of post-thawed sperm in the three species, as well as spermatozoan motility in A. sinensis. The combination of antioxidants did not show a positive synergistic effect. This study suggested that the use of antioxidants in the cryopreservation of sturgeon sperm has potential to decrease intracellular ROS, and consequently preserve acrosome and membrane integrity, as well as spermatozoan motility.

Key Laboratory of Freshwater Biodiversity Conservation Ministry of Agriculture of China Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences Wuhan China Sino Czech Joint Laboratory for Fish Conservation and Biotechnology Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences Wuhan China

Key Laboratory of Freshwater Biodiversity Conservation Ministry of Agriculture of China Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences Wuhan China Sino Czech Joint Laboratory for Fish Conservation and Biotechnology Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences Wuhan China Faculty of Fisheries and Protection of Waters Research Institute of Fish Culture and Hydrobiology University of South Bohemia in České Budějovice South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Vodňany Czech Republic

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