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Study of non-covalent interactions on dendriplex formation: Influence of hydrophobic, electrostatic and hydrogen bonds interactions

M. Sánchez-Milla, I. Pastor, M. Maly, MJ. Serramía, R. Gómez, J. Sánchez-Nieves, F. Ritort, MÁ. Muñoz-Fernández, FJ. de la Mata,

. 2018 ; 162 (-) : 380-388. [pub] 20171208

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18033466

The interaction of a double stranded small interference RNA (siRNA Nef) with cationic carbosilane dendrimers of generations 1-3 with two different ammonium functions at the periphery ([-NMe2R]+, R=Me, (CH2)2OH) has been studied by experimental techniques (zeta potential, electrophoresis, single molecule pulling experiments) and molecular dynamic calculations. These studies state the presence of different forces on dendriplex formation, depending on generation and type of ammonium group. Whilst for higher dendrimers electrostatic forces mainly drive the stability of dendriplexes, first generation compounds can penetrate into siRNA strands due to the establishment of hydrophobic interactions. Finally, in the particular case of first generation dendrimer [G1O3(NMe2(CH2)2OH))6]6+; the presence of hydroxyl groups reinforces dendriplex stability by hydrogen bonds formation. However, since these small dendrimers do not cover the RNA, only higher generation derivatives protect RNA from degradation.

Citace poskytuje Crossref.org

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$a The interaction of a double stranded small interference RNA (siRNA Nef) with cationic carbosilane dendrimers of generations 1-3 with two different ammonium functions at the periphery ([-NMe2R]+, R=Me, (CH2)2OH) has been studied by experimental techniques (zeta potential, electrophoresis, single molecule pulling experiments) and molecular dynamic calculations. These studies state the presence of different forces on dendriplex formation, depending on generation and type of ammonium group. Whilst for higher dendrimers electrostatic forces mainly drive the stability of dendriplexes, first generation compounds can penetrate into siRNA strands due to the establishment of hydrophobic interactions. Finally, in the particular case of first generation dendrimer [G1O3(NMe2(CH2)2OH))6]6+; the presence of hydroxyl groups reinforces dendriplex stability by hydrogen bonds formation. However, since these small dendrimers do not cover the RNA, only higher generation derivatives protect RNA from degradation.
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$a Pastor, Isabel $u Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain; Small Biosystems Lab, Condensed Matter Physics Department, Universitat de Barcelona, C/Martí i Franquès, E-08028, Barcelona, Spain.
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$a Maly, Marek $u Faculty of Science, J. E. Purkinje University, Ceske mladeze 8, 400 96 Usti nad Labem, Czech Republic.
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$a Serramía, M Jesús $u Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain; Sección Inmunología, Laboratorio de InmunoBiología Molecular, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; Spanish HIV HGM BioBank, Madrid, Spain.
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$a Gómez, Rafael $u Department of Química Orgánica y Química Inorgánica, Universidad de Alcalá (IRYCIS), Campus Universitario, E-28871 Alcalá de Henares, Madrid, Spain; Instituto de Investigación Química "Andrés M. del Río" (IQAR), Universidad de Alcalá, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain.
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$a Sánchez-Nieves, Javier $u Department of Química Orgánica y Química Inorgánica, Universidad de Alcalá (IRYCIS), Campus Universitario, E-28871 Alcalá de Henares, Madrid, Spain; Instituto de Investigación Química "Andrés M. del Río" (IQAR), Universidad de Alcalá, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain. Electronic address: javier.sancheznieves@uah.es.
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$a Ritort, Félix $u Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain; Small Biosystems Lab, Condensed Matter Physics Department, Universitat de Barcelona, C/Martí i Franquès, E-08028, Barcelona, Spain.
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$a Muñoz-Fernández, M Ángeles $u Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain; Sección Inmunología, Laboratorio de InmunoBiología Molecular, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; Spanish HIV HGM BioBank, Madrid, Spain.
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$a de la Mata, F Javier $u Department of Química Orgánica y Química Inorgánica, Universidad de Alcalá (IRYCIS), Campus Universitario, E-28871 Alcalá de Henares, Madrid, Spain; Instituto de Investigación Química "Andrés M. del Río" (IQAR), Universidad de Alcalá, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain. Electronic address: javier.delamata@uah.es.
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