Proper maturation of the mammalian oocyte is a compound processes determining successful monospermic fertilization, however the number of fully mature porcine oocytes is still unsatisfactory. Since oocytes' maturation and fertilization involve cellular adhesion and membranous contact, the aim was to investigate cell adhesion ontology group in porcine oocytes. The oocytes were collected from ovaries of 45 pubertal crossbred Landrace gilts and subjected to two BCB tests. After the first test, only granulosa cell-free BCB⁺ oocytes were directly exposed to microarray assays and RT-qPCR ("before IVM" group), or first in vitro matured and then if classified as BCB⁺ passed to molecular analyses ("after IVM" group). As a result, we have discovered substantial down-regulation of genes involved in adhesion processes, such as: organization of actin cytoskeleton, migration, proliferation, differentiation, apoptosis, survival or angiogenesis in porcine oocytes after IVM, compared to oocytes analyzed before IVM. In conclusion, we found that biological adhesion may be recognized as the process involved in porcine oocytes' successful IVM. Down-regulation of genes included in this ontology group in immature oocytes after IVM points to their unique function in oocyte's achievement of fully mature stages. Thus, results indicated new molecular markers involved in porcine oocyte IVM, displaying essential roles in biological adhesion processes.

Department of Anatomy Poznan University of Medical Sciences 60 781 Poznan Poland

Department of Anatomy Poznan University of Medical Sciences 60 781 Poznan Poland Department of Biomaterials and Experimental Dentistry Poznan University of Medical Sciences 60 812 Poznan Poland

Department of Histology and Embryology Poznan University of Medical Sciences 60 781 Poznan Poland

Department of Histology and Embryology Poznan University of Medical Sciences 60 781 Poznan Poland Department of Anatomy Poznan University of Medical Sciences 60 781 Poznan Poland Department of Obstetrics and Gynecology University Hospital and Masaryk University 602 00 Brno Czech Republic

Department of Histology and Embryology Poznan University of Medical Sciences 60 781 Poznan Poland Department of Histology and Embryology Wroclaw Medical University 50 368 Wroclaw Poland

Department of Obstetrics and Gynecology University Hospital and Masaryk University 602 00 Brno Czech Republic

Veterinary Center Nicolaus Copernicus University in Toruń 87 100 Torun Poland

000      

00000naa a2200000 a 4500

001      

bmc18033473

003      

CZ-PrNML

005      

20181010124918.0

007      

ta

008      

181008s2017 sz f 000 00|eng||

009      

AR

024    7_

$a 10.3390/ijms18122685 $2 doi

035    __

$a (PubMed)29232894

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a sz

100    1_

$a Budna, Joanna $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland. joanna.budna@wp.pl.

245    10

$a Significant Down-Regulation of "Biological Adhesion" Genes in Porcine Oocytes after IVM / $c J. Budna, P. Celichowski, A. Bryja, M. Dyszkiewicz-Konwińska, M. Jeseta, D. Bukowska, P. Antosik, KP. Brüssow, M. Bruska, M. Nowicki, M. Zabel, B. Kempisty,

520    9_

$a Proper maturation of the mammalian oocyte is a compound processes determining successful monospermic fertilization, however the number of fully mature porcine oocytes is still unsatisfactory. Since oocytes' maturation and fertilization involve cellular adhesion and membranous contact, the aim was to investigate cell adhesion ontology group in porcine oocytes. The oocytes were collected from ovaries of 45 pubertal crossbred Landrace gilts and subjected to two BCB tests. After the first test, only granulosa cell-free BCB⁺ oocytes were directly exposed to microarray assays and RT-qPCR ("before IVM" group), or first in vitro matured and then if classified as BCB⁺ passed to molecular analyses ("after IVM" group). As a result, we have discovered substantial down-regulation of genes involved in adhesion processes, such as: organization of actin cytoskeleton, migration, proliferation, differentiation, apoptosis, survival or angiogenesis in porcine oocytes after IVM, compared to oocytes analyzed before IVM. In conclusion, we found that biological adhesion may be recognized as the process involved in porcine oocytes' successful IVM. Down-regulation of genes included in this ontology group in immature oocytes after IVM points to their unique function in oocyte's achievement of fully mature stages. Thus, results indicated new molecular markers involved in porcine oocyte IVM, displaying essential roles in biological adhesion processes.

650    _2

$a zvířata $7 D000818

650    _2

$a apoptóza $7 D017209

650    _2

$a buněčná adheze $7 D002448

650    _2

$a buněčná diferenciace $7 D002454

650    _2

$a proliferace buněk $7 D049109

650    12

$a down regulace $7 D015536

650    _2

$a ženské pohlaví $7 D005260

650    12

$a genové regulační sítě $7 D053263

650    _2

$a IVM techniky $x veterinární $7 D059471

650    _2

$a oocyty $x cytologie $x metabolismus $7 D009865

650    _2

$a oogeneze $7 D009866

650    _2

$a prasata $7 D013552

655    _2

$a časopisecké články $7 D016428

700    1_

$a Celichowski, Piotr $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland. pcelichowski@ump.edu.pl.

700    1_

$a Bryja, Artur $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland. abryja@ump.edu.pl.

700    1_

$a Dyszkiewicz-Konwińska, Marta $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland. mdyszkiewicz@ump.edu.pl. Department of Biomaterials and Experimental Dentistry, Poznan University of Medical Sciences, 60-812 Poznan, Poland. mdyszkiewicz@ump.edu.pl.

700    1_

$a Jeseta, Michal $u Department of Obstetrics and Gynecology, University Hospital and Masaryk University, 602 00 Brno, Czech Republic. jeseta@gmail.com.

700    1_

$a Bukowska, Dorota $u Veterinary Center, Nicolaus Copernicus University in Toruń, 87-100 Torun, Poland. lecznicapaw@gmail.com.

700    1_

$a Antosik, Paweł $u Veterinary Center, Nicolaus Copernicus University in Toruń, 87-100 Torun, Poland. antosik67@wp.pl.

700    1_

$a Brüssow, Klaus Peter $u Veterinary Center, Nicolaus Copernicus University in Toruń, 87-100 Torun, Poland. prof.bruessow@gmail.com.

700    1_

$a Bruska, Małgorzata $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland. bruska@ump.edu.pl.

700    1_

$a Nowicki, Michał $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland. mnowicki@ump.edu.pl.

700    1_

$a Zabel, Maciej $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland. mazab@ump.edu.pl. Department of Histology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland. mazab@ump.edu.pl.

700    1_

$a Kempisty, Bartosz $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland. bkempisty@ump.edu.pl. Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland. bkempisty@ump.edu.pl. Department of Obstetrics and Gynecology, University Hospital and Masaryk University, 602 00 Brno, Czech Republic. bkempisty@ump.edu.pl.

773    0_

$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 18, č. 12 (2017)

856    41

$u https://pubmed.ncbi.nlm.nih.gov/29232894 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20181008 $b ABA008

991    __

$a 20181010125407 $b ABA008

999    __

$a ok $b bmc $g 1340921 $s 1030467

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2017 $b 18 $c 12 $e 20171211 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142

LZP    __

$a Pubmed-20181008